Clone BO12068 Report

Search the DGRC for BO12068

Clone and Library Details

Library:BO
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with open reading frames
Original Plate Number:120
Well:68
Vector:pDNR-Dual
Associated Gene/TranscriptMctp-RB
Protein status:BO12068.pep:
Sequenced Size:1822

Clone Sequence Records

BO12068.3prime Sequence

906 bp (820 high quality bases) assembled on 2006-04-18

> BO12068.3prime
ATGGTCTAGAAAGCTTGCTGATCCTTTCAGTTTCTTCTTGGGATTGTTGC
GCGTCTGATCGGTGGGTGCCGATGGTGGCAGCTCTCGATATTGATTAATC
TCCTCATTATCTGGCACTCGCGAGAGGAAGTCCAATAGCTCGTTGTTGGG
AATGGTATTGGGTCTCAGCAAACGTCTGGAGAACTTCATCAGGCCCCAAA
AGAGCAGCAGCCAGCGGAGCGGGACAAAATGCAAGACCAGAATGGCGCCC
AATAGCAAAACCACAGCCAGCCAGGTCAGTTCGGGGACGGAAAAGTTGAA
TGTGTTAATGGTGCTCTCGCCTAAAGAAGCCAGGTAGCCGATGGTATTCT
GTACCGTTTGGGAGACTTCTTGAATGGCCTGTAATCTCTCCTTGATGGAC
TTCTTTTCCTCCTTCTCCTTGTCATCATCATCATCTTCATCGTACTCATA
GTCGTAGTGAGCAGCTGCATCTGTGGTGCCCGTTATAAGGCGAACCAGCC
AGTTTTTAAGTATGATGAGCAGTAGAACTAGAGGTACTGTCTCCAGATCG
CCGTAGACACAGGCGACTATCCAAAAGACGAAAGCTATGCTGGATCGCAC
AGGAGATTCCCACTCGAAACAGCTCTGGACATAGCGCGCTGCATCTAGGA
TATCCATGATTATCTCCTTGAGTCTATTGACGTTGCGCAGGAAGAGCTGT
CGCTTGAACTTGGCCTCCTGCTGGATGAGCTTTTCCTCCTTGGGCTGCAG
GGCTCGACACACGGCACGGATCTCGCTCCAGACTACCGTCAGTTCCAGCT
GAATTTGCGGTGAGTTTCCTTTGGCCCGCACACAGAGATTCTTGTCCTTC
AGCGTATACCATCGCTTGACTCCACTTTTGATCTTTAGGAGGGGAATGAC
TAGCTT

BO12068.3prime Blast Records

Blast to dmel-all-CDS-r5.1.fasta performed 2007-05-06 14:36:26
Subject Length Description Subject Range Query Range Score Percent Strand
CG15078-PA 2790 Mctp-RA 1900..2787 906..19 4415 99.8 Minus
CG15078-PB 1791 Mctp-RB 901..1788 906..19 4415 99.8 Minus
CG15078-PC 2823 Mctp-RC 1933..2820 906..19 4415 99.8 Minus
Blast to dmel-all-transcript-r6.02.fasta performed 2015-02-12 12:10:14
Subject Length Description Subject Range Query Range Score Percent Strand
Mctp-RG 4123 CG15078-RG 2309..3196 906..19 4425 99.9 Minus
Mctp-RF 4223 CG15078-RF 2252..3139 906..19 4425 99.9 Minus
Mctp-RE 4015 CG15078-RE 2201..3088 906..19 4425 99.9 Minus
Blast to na_all.dmel.RELEASE6 performed 2015-02-12 12:10:08
Subject Length Description Subject Range Query Range Score Percent Strand
2R 25286936 2R 18772618..18772899 906..625 1395 99.6 Minus
2R 25286936 2R 18773398..18773605 304..97 1040 100 Minus
2R 25286936 2R 18773131..18773335 509..305 1010 99.5 Minus
2R 25286936 2R 18772958..18773077 625..506 600 100 Minus
2R 25286936 2R 18773663..18773740 96..19 390 100 Minus
Blast to na_te.dros performed on 2015-02-12 12:10:11 has no hits.

BO12068.3prime Sim4 Records

Sim4 to dmel-all-CDS-r4.3.fasta performed 2007-01-18 05:25:03 Download gff for BO12068.3prime
Subject Subject Range Query Range Percent Splice Strand
CG33146-PA 901..1791 18..906 99   Minus
Sim4 to dmel-all-CDS-r5.1.fasta performed 2007-05-10 04:56:09 Download gff for BO12068.3prime
Subject Subject Range Query Range Percent Splice Strand
CG15078-PC 1933..2823 18..906 99   Minus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-30 03:01:55 Download gff for BO12068.3prime
Subject Subject Range Query Range Percent Splice Strand
Mctp-RB 1010..1897 19..906 99   Minus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2015-02-12 13:57:20 Download gff for BO12068.3prime
Subject Subject Range Query Range Percent Splice Strand
Mctp-RE 2201..3088 19..906 99   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2015-02-12 13:57:20 Download gff for BO12068.3prime
Subject Subject Range Query Range Percent Splice Strand
2R 18772618..18772898 626..906 99 -> Minus
2R 18772958..18773077 506..625 100 -> Minus
2R 18773135..18773335 305..505 100 -> Minus
2R 18773398..18773605 97..304 100 -> Minus
2R 18773663..18773740 19..96 100   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-30 03:01:55 Download gff for BO12068.3prime
Subject Subject Range Query Range Percent Splice Strand
arm_2R 14660123..14660403 626..906 99 -> Minus
arm_2R 14660463..14660582 506..625 100 -> Minus
arm_2R 14660640..14660840 305..505 100 -> Minus
arm_2R 14660903..14661110 97..304 100 -> Minus
arm_2R 14661168..14661245 19..96 100   Minus

BO12068.5prime Sequence

903 bp (812 high quality bases) assembled on 2006-04-18

> BO12068.5prime
GAAGTTATCAGTCGACATGTTGGGCAACAGTTGGCCCGGTTGCAAGCGGG
CTCCTCGCATGGTGCTCAAACATGTGAGGCGGCAGCACGAGGAGTTGCGG
GAGCAGCTGGAGCAGGGATGCGACGATCTCATGGAGGTGGCCGAGTCTTA
TTTTCCGGATGTCTTGCAGCATTTCCAGCGCAACTCGAAACTGGCTGAGT
CATCGAAGCGATTGAAGTCACAGATCTGGAGCTCAGTGGTTACCATATTG
CTGGTTAAAGCCAAGGATTTGCCCTTAGCTGAAGATGGCAGCAAGCTGAA
TGATACTCACTTCAAATTCAGATTGGGCAATGAGAAGTACAAGAGCAAGT
CTTCCTGGACGGAACGCTGGCTGGAGCAGTTCGACTTGCACCTGTTTGAT
GAAGATCAAAACCTGGAGATCGCACTTTGGAACCGGAACACCCTCTATGG
CAAAGCCATTATTGACCTCAGCGTTTTTCAGCGCGAAAACACGCATGGCA
TTTGGAAACCTCTGGAAGATTGCCCTGGTGAGGTCCACCTTATGCTCACT
ATAAGTGGAACCACTGCCTTGGAGACGATCAGCGATCTGAAGGCCTTCAA
GGAGGATCCTCGAGAGGCTCAATTGCTAAGGGAAAGATATAAATTCCTAA
GATGCCTCCAAAACCTCCGCGATGTGGGTCACCTAACTGTAAAGGTCTTT
GGAGCCACTGGACTGGCTGCAGCGGATATAGGTGGAAAATCCGATCCTTT
TTGTGTATTGGAACTGGGTAACGCCCGACTGCAAACCCAAACTGAGTATA
AAACACTCACTCCTAATTGGAATAAGATATTTACTTTTCATGTTAAAGAC
ATCACGCAGGTCCTGGAGATCACCGTTTTCGATGAGGATCGCGACCATCG
CGT

BO12068.5prime Blast Records

Blast to dmel-all-CDS-r5.1.fasta performed 2007-05-06 14:36:26
Subject Length Description Subject Range Query Range Score Percent Strand
CG15078-PB 1791 Mctp-RB 1..887 17..903 4385 99.7 Plus
CG15078-PC 2823 Mctp-RC 1152..1919 136..903 3790 99.7 Plus
CG15078-PA 2790 Mctp-RA 1152..1886 169..903 3625 99.7 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2015-02-04 05:35:21
Subject Length Description Subject Range Query Range Score Percent Strand
Mctp-RB 2824 CG15078-RB 109..996 16..903 4410 99.8 Plus
Mctp-RG 4123 CG15078-RG 1561..2295 169..903 3645 99.7 Plus
Mctp-RF 4223 CG15078-RF 1504..2238 169..903 3645 99.7 Plus
Blast to na_all.dmel.RELEASE6 performed 2015-02-04 05:35:16
Subject Length Description Subject Range Query Range Score Percent Strand
2R 25286936 2R 18771950..18772470 317..837 2605 100 Plus
2R 25286936 2R 18771586..18771891 16..321 1530 100 Plus
2R 25286936 2R 18772541..18772604 840..903 320 100 Plus
Blast to na_te.dros performed 2015-02-04 05:35:19
Subject Length Description Subject Range Query Range Score Percent Strand
gtwin 7411 gtwin GTWIN 7411bp 1756..1796 246..286 115 75.6 Plus

BO12068.5prime Sim4 Records

Sim4 to dmel-all-CDS-r4.3.fasta performed 2007-01-18 05:25:04 Download gff for BO12068.5prime
Subject Subject Range Query Range Percent Splice Strand
CG33146-PA 1..887 17..903 99   Plus
Sim4 to dmel-all-CDS-r5.1.fasta performed 2007-05-10 04:56:10 Download gff for BO12068.5prime
Subject Subject Range Query Range Percent Splice Strand
CG15078-PB 1..887 17..903 99   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-10 07:15:31 Download gff for BO12068.5prime
Subject Subject Range Query Range Percent Splice Strand
Mctp-RB 109..996 16..903 99   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2015-02-04 06:47:13 Download gff for BO12068.5prime
Subject Subject Range Query Range Percent Splice Strand
Mctp-RB 109..996 16..903 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2015-02-04 06:47:13 Download gff for BO12068.5prime
Subject Subject Range Query Range Percent Splice Strand
2R 18771586..18771891 16..321 100 -> Plus
2R 18771955..18772470 322..837 100 -> Plus
2R 18772539..18772604 838..903 96   Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-10 07:15:31 Download gff for BO12068.5prime
Subject Subject Range Query Range Percent Splice Strand
arm_2R 14660044..14660109 838..903 96   Plus
arm_2R 14659091..14659396 16..321 100 -> Plus
arm_2R 14659460..14659975 322..837 100 -> Plus

BO12068.complete Sequence

1822 bp assembled on 2008-01-17

GenBank Submission: FJ632298

> BO12068.complete
GAAGTTATCAGTCGACATGTTGGGCAACAGTTGGCCCGGTTGCAAGCGGG
CTCCTCGCATGGTGCTCAAACATGTGAGGCGGCAGCACGAGGAGTTGCGG
GAGCAGCTGGAGCAGGGATGCGACGATCTCATGGAGGTGGCCGAGTCTTA
TTTTCCGGATGTCTTGCAGCATTTCCAGCGCAACTCGAAACTGGCTGAGT
CATCGAAGCGATTGAAGTCACAGATCTGGAGCTCAGTGGTTACCATATTG
CTGGTTAAAGCCAAGGATTTGCCCTTAGCTGAAGATGGCAGCAAGCTGAA
TGATACTCACTTCAAATTCAGATTGGGCAATGAGAAGTACAAGAGCAAGT
CTTCCTGGACGGAACGCTGGCTGGAGCAGTTCGACTTGCACCTGTTTGAT
GAAGATCAAAACCTGGAGATCGCACTTTGGAACCGGAACACCCTCTATGG
CAAAGCCATTATTGACCTCAGCGTTTTTCAGCGCGAAAACACGCATGGCA
TTTGGAAACCTCTGGAAGATTGCCCTGGTGAGGTCCACCTTATGCTCACT
ATAAGTGGAACCACTGCCTTGGAGACGATCAGCGATCTGAAGGCCTTCAA
GGAGGATCCTCGAGAGGCTCAATTGCTAAGGGAAAGATATAAATTCCTAA
GATGCCTCCAAAACCTCCGCGATGTGGGTCACCTAACTGTAAAGGTCTTT
GGAGCCACTGGACTGGCTGCAGCGGATATAGGTGGAAAATCCGATCCTTT
TTGTGTATTGGAACTGGGTAACGCCCGACTGCAAACCCAAACTGAGTATA
AAACACTCACTCCTAATTGGAATAAGATATTTACTTTCAATGTTAAAGAC
ATCACGCAGGTCCTGGAGATCACCGTTTTCGATGAGGATCGCGACCATCG
CGTAGAGTTCCTGGGCAAGCTAGTCATTCCCCTCCTAAGGATCAAAAGTG
GAGTCAAGCGATGGTATACGCTGAAGGACAAGAATCTCTGTGTGCGGGCC
AAAGGAAACTCACCGCAAATTCAGCTGGAACTGACGGTAGTCTGGAGCGA
GATCCGTGCCGTGTGTCGAGCCCTGCAGCCCAAGGAGGAAAAGCTCATCC
AGCAGGAGGCCAAGTTCAAGCGACAGCTCTTCCTGCGCAACGTCAATAGA
CTCAAGGAGATAATCATGGATATCCTAGATGCAGCGCGCTATGTCCAGAG
CTGTTTCGAGTGGGAATCTCCTGTGCGATCCAGCATAGCTTTCGTCTTTT
GGATAGTCGCCTGTGTCTACGGCGATCTGGAGACAGTACCTCTAGTTCTA
CTGCTCATCATACTTAAAAACTGGCTGGTTCGCCTTATAACGGGCACCAC
AGATGCAGCTGCTCACTACGACTATGAGTACGATGAAGATGATGATGATG
ACAAGGAGAAGGAGGAAAAGAAGTCCATCAAGGAGAGATTACAGGCCATT
CAAGAAGTCTCCCAAACGGTACAGAATACCATCGGCTACCTGGCTTCTTT
AGGCGAGAGCACCATTAACACATTCAACTTTTCCGTCCCCGAACTGACCT
GGCTGGCTGTGGTTTTGCTATTGGGCGCCATTCTGGTCTTGCATTTTGTC
CCGCTCCGCTGGCTGCTGCTCTTTTGGGGCCTGATGAAGTTCTCCAGACG
TTTGCTGAGACCCAATACCATTCCCAACAACGAGCTATTGGACTTCCTCT
CGCGAGTGCCAGATAATGAGGAGATTAATCAATATCGAGAGCTGCCACCA
TCGGCACCCACCGATCAGACGCGCAACAATCCCAAGAAGAAACTGAAAGG
ATCAGCAAGCTTTCTAGACCAT

BO12068.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-28 00:58:22
Subject Length Description Subject Range Query Range Score Percent Strand
Mctp-RB 1791 CG15078-PB 1..1788 17..1804 8940 100 Plus
Mctp-RG 2739 CG15078-PG 1101..2736 169..1804 8180 100 Plus
Mctp-RF 2682 CG15078-PF 1044..2679 169..1804 8180 100 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-28 00:58:23
Subject Length Description Subject Range Query Range Score Percent Strand
Mctp-RB 2824 CG15078-RB 109..1897 16..1804 8945 100 Plus
Mctp-RG 4123 CG15078-RG 1561..3196 169..1804 8180 100 Plus
Mctp-RF 4223 CG15078-RF 1504..3139 169..1804 8180 100 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-28 00:58:19
Subject Length Description Subject Range Query Range Score Percent Strand
2R 25286936 2R 18771950..18772470 317..837 2605 100 Plus
2R 25286936 2R 18772539..18772899 838..1198 1805 100 Plus
2R 25286936 2R 18771586..18771891 16..321 1530 100 Plus
2R 25286936 2R 18773398..18773605 1519..1726 1040 100 Plus
2R 25286936 2R 18773131..18773335 1314..1518 1010 99.5 Plus
2R 25286936 2R 18772958..18773077 1198..1317 600 100 Plus
2R 25286936 2R 18773663..18773740 1727..1804 390 100 Plus
Blast to na_te.dros performed 2014-11-28 00:58:21
Subject Length Description Subject Range Query Range Score Percent Strand
gtwin 7411 gtwin GTWIN 7411bp 1756..1796 246..286 115 75.6 Plus

BO12068.complete Sim4 Records

Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-21 13:59:48 Download gff for BO12068.complete
Subject Subject Range Query Range Percent Splice Strand
Mctp-RB 1..1791 17..1805 99   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2009-07-28 15:34:10 Download gff for BO12068.complete
Subject Subject Range Query Range Percent Splice Strand
Mctp-RB 104..1886 22..1806 99   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 20:35:26 Download gff for BO12068.complete
Subject Subject Range Query Range Percent Splice Strand
Mctp-RB 115..1897 22..1806 99   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-21 13:59:48 Download gff for BO12068.complete
Subject Subject Range Query Range Percent Splice Strand
Mctp-RB 98..1886 16..1804 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-28 01:41:26 Download gff for BO12068.complete
Subject Subject Range Query Range Percent Splice Strand
Mctp-RB 115..1897 22..1806 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-28 01:41:26 Download gff for BO12068.complete
Subject Subject Range Query Range Percent Splice Strand
2R 18771592..18771891 22..321 100 -> Plus
2R 18771955..18772470 322..837 100 -> Plus
2R 18772539..18772898 838..1197 100 -> Plus
2R 18772958..18773077 1198..1317 100 -> Plus
2R 18773135..18773335 1318..1518 100 -> Plus
2R 18773398..18773605 1519..1726 100 -> Plus
2R 18773663..18773740 1727..1806 97   Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 20:35:26 Download gff for BO12068.complete
Subject Subject Range Query Range Percent Splice Strand
arm_2R 14659097..14659396 22..321 100 -> Plus
arm_2R 14659460..14659975 322..837 100 -> Plus
arm_2R 14660044..14660403 838..1197 100 -> Plus
arm_2R 14660463..14660582 1198..1317 100 -> Plus
arm_2R 14660640..14660840 1318..1518 100 -> Plus
arm_2R 14660903..14661110 1519..1726 100 -> Plus
arm_2R 14661168..14661245 1727..1806 97   Plus

BO12068.pep Sequence

Translation from 16 to 1822

> BO12068.pep
MLGNSWPGCKRAPRMVLKHVRRQHEELREQLEQGCDDLMEVAESYFPDVL
QHFQRNSKLAESSKRLKSQIWSSVVTILLVKAKDLPLAEDGSKLNDTHFK
FRLGNEKYKSKSSWTERWLEQFDLHLFDEDQNLEIALWNRNTLYGKAIID
LSVFQRENTHGIWKPLEDCPGEVHLMLTISGTTALETISDLKAFKEDPRE
AQLLRERYKFLRCLQNLRDVGHLTVKVFGATGLAAADIGGKSDPFCVLEL
GNARLQTQTEYKTLTPNWNKIFTFNVKDITQVLEITVFDEDRDHRVEFLG
KLVIPLLRIKSGVKRWYTLKDKNLCVRAKGNSPQIQLELTVVWSEIRAVC
RALQPKEEKLIQQEAKFKRQLFLRNVNRLKEIIMDILDAARYVQSCFEWE
SPVRSSIAFVFWIVACVYGDLETVPLVLLLIILKNWLVRLITGTTDAAAH
YDYEYDEDDDDDKEKEEKKSIKERLQAIQEVSQTVQNTIGYLASLGESTI
NTFNFSVPELTWLAVVLLLGAILVLHFVPLRWLLLFWGLMKFSRRLLRPN
TIPNNELLDFLSRVPDNEEINQYRELPPSAPTDQTRNNPKKKLKGSASFL
DH

BO12068.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 15:21:37
Subject Length Description Subject Range Query Range Score Percent Strand
Mctp-PB 596 CG15078-PB 1..596 1..596 3108 100 Plus
Mctp-PD 982 CG15078-PD 364..914 48..598 2835 99.3 Plus
Mctp-PG 912 CG15078-PG 364..912 48..596 2832 99.5 Plus
Mctp-PF 893 CG15078-PF 345..893 48..596 2832 99.5 Plus
Mctp-PE 954 CG15078-PE 406..954 48..596 2832 99.5 Plus