Clone BS02528 Report

Search the DGRC for BS02528

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:25
Well:28
Vector:pDNR-Dual
Associated Gene/Transcriptkni-RA
Protein status:BS02528.pep: full length peptide match
Sequenced Size:16

Clone Sequence Records

BS02528.complete Sequence

1324 bp (1324 high quality bases) assembled on 2005-10-17

GenBank Submission: FJ634669

> BS02528.complete
GAAGTTATCAGTCGACATGAACCAGACATGCAAAGTGTGCGGTGAGCCGG
CGGCGGGCTTCCATTTTGGCGCCTTCACCTGCGAGGGCTGCAAGTCCTTC
TTTGGCCGCTCTTACAACAACATCAGCACCATCAGCGAGTGCAAGAACGA
GGGCAAGTGCATCATCGACAAGAAGAACCGCACCACCTGCAAGGCGTGCC
GCTTGAGGAAGTGCTACAACGTGGGCATGTCGAAGGGGGGATCCCGCTAC
GGACGTCGCTCCAACTGGTTCAAGATCCATTGTCTGCTGCAGGAGCACGA
ACAGGCCGCCGCAGCGGCGGGCAAGGCGCCTCCATTAGCGGGTGGCGTAT
CGGTGGGTGGTGCCCCGTCGGCCTCTTCCCCGGTGGGCTCGCCACACACT
CCCGGATTTGGGGACATGGCCGCCCATTTGCACCACCATCATCAGCAGCA
GCAGCAGCAGCAGGTGCCGCGTCATCCACATATGCCTCTGCTGGGCTATC
CCAGCTATCTGTCCGACCCATCCGCCGCCCTGCCCTTCTTCAGCATGATG
GGCGGTGTACCGCACCAGTCGCCCTTCCAGCTGCCCCCACACCTCCTCTT
CCCAGGCTACCATGCAAGTGCTGCCGCTGCAGCGGCTTCTGCTGCCGATG
CCGCTTACCGGCAGGAGATGTACAAGCACCGCCAGAGCGTGGATTCCGTT
GAGTCGCAGAACCGCTTTAGTCCCGCCAGCCAGCCACCAGTGGTGCAGCC
CACCTCCTCGGCCCGCCAGTCGCCCATCGATGTCTGCCTGGAGGAGGATG
TTCACTCCGTGCACAGCCATCAGTCGTCCGCAAGCCTCCTGCATCCCATT
GCCATCCGAGCCACGCCAACCACTCCGACTAGCAGCAGCCCGCTGAGTTT
TGCGGCCAAGATGCAGAGCTTGTCGCCCGTTTCGGTTTGCTCCATTGGCG
GCGAAACCACCAGCGTTGTACCAGTGCATCCTCCCACCGTTTCCGCTCAA
GAAGGACCCATGGATCTGAGCATGAAGACCTCGCGGAGCTCCGTGCACAG
CTTCAACGACAGCGGCTCCGAGGATCAAGAAGTGGAGGTGGCTCCGCGCC
GGAAGTTCTACCAACTGGAGGCCGAGTGCCTGACCACCAGCAGCAGCAGT
TCCTCCCACTCCGCCGCCCACTCACCGAACACCACCACCGCCCATGCGGA
AGTCAAGCGGCAGAAGCTAGGTGGTGCAGAGGCCACCCACTTCGGTGGCT
TCGCGGTGGCCCACAATGCGGCTAGTGCCATGAGGGGAATATTCGTGTGT
GTCTAACGAAGCTTTCTAGACCAT

BS02528.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-26 21:39:49
Subject Length Description Subject Range Query Range Score Percent Strand
kni-RB 1305 CG4717-PB 16..1305 17..1306 6450 100 Plus
kni-RA 1290 CG4717-PA 1..1290 17..1306 6450 100 Plus
knrl-RB 1944 CG4761-PB 28..308 17..297 730 84 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-26 21:39:50
Subject Length Description Subject Range Query Range Score Percent Strand
kni-RB 1911 CG4717-RB 116..1405 17..1306 6450 100 Plus
kni-RA 2100 CG4717-RA 305..1594 17..1306 6450 100 Plus
knrl-RB 2925 CG4761-RB 992..1272 17..297 730 84 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-26 21:39:46
Subject Length Description Subject Range Query Range Score Percent Strand
3L 28110227 3L 20692836..20694049 1306..93 6070 100 Minus
3L 28110227 3L 20599778..20599982 297..93 515 83.4 Minus
3L 28110227 3L 20694263..20694340 94..17 390 100 Minus
3L 28110227 3L 21816347..21816480 164..297 355 84.3 Plus
3L 28110227 3L 20602193..20602270 94..17 225 85.9 Minus
3L 28110227 3L 21814779..21814856 17..94 195 83.3 Plus
Blast to na_te.dros performed 2014-11-26 21:39:47
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2443..2519 113..192 150 67.5 Plus
Dmer\R1A3 3772 Dmer\R1A3 MERCR1A3 3772bp 596..728 523..650 113 60.6 Plus

BS02528.complete Sim4 Records

Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-22 00:29:54 Download gff for BS02528.complete
Subject Subject Range Query Range Percent Splice Strand
kni-RA 1..1290 17..1306 100   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2008-11-11 04:13:22 Download gff for BS02528.complete
Subject Subject Range Query Range Percent Splice Strand
kni-RA 290..1579 17..1306 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 03:32:57 Download gff for BS02528.complete
Subject Subject Range Query Range Percent Splice Strand
kni-RA 305..1595 17..1308 99   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-22 00:29:54 Download gff for BS02528.complete
Subject Subject Range Query Range Percent Splice Strand
kni-RA 290..1579 17..1306 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-26 22:38:58 Download gff for BS02528.complete
Subject Subject Range Query Range Percent Splice Strand
kni-RA 305..1595 17..1308 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-26 22:38:58 Download gff for BS02528.complete
Subject Subject Range Query Range Percent Splice Strand
3L 20692835..20694047 95..1308 99 <- Minus
3L 20694263..20694340 17..94 100   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 03:32:57 Download gff for BS02528.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3L 20685935..20687147 95..1308 99 <- Minus
arm_3L 20687363..20687440 17..94 100   Minus

BS02528.pep Sequence

Translation from 16 to 1305

> BS02528.pep
MNQTCKVCGEPAAGFHFGAFTCEGCKSFFGRSYNNISTISECKNEGKCII
DKKNRTTCKACRLRKCYNVGMSKGGSRYGRRSNWFKIHCLLQEHEQAAAA
AGKAPPLAGGVSVGGAPSASSPVGSPHTPGFGDMAAHLHHHHQQQQQQQV
PRHPHMPLLGYPSYLSDPSAALPFFSMMGGVPHQSPFQLPPHLLFPGYHA
SAAAAAASAADAAYRQEMYKHRQSVDSVESQNRFSPASQPPVVQPTSSAR
QSPIDVCLEEDVHSVHSHQSSASLLHPIAIRATPTTPTSSSPLSFAAKMQ
SLSPVSVCSIGGETTSVVPVHPPTVSAQEGPMDLSMKTSRSSVHSFNDSG
SEDQEVEVAPRRKFYQLEAECLTTSSSSSSHSAAHSPNTTTAHAEVKRQK
LGGAEATHFGGFAVAHNAASAMRGIFVCV*

BS02528.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 15:30:26
Subject Length Description Subject Range Query Range Score Percent Strand
kni-PB 434 CG4717-PB 6..434 1..429 2276 100 Plus
kni-PA 429 CG4717-PA 1..429 1..429 2276 100 Plus
knrl-PB 647 CG4761-PB 10..128 1..116 503 78.2 Plus
knrl-PA 647 CG4761-PA 10..128 1..116 503 78.2 Plus
eg-PA 373 CG7383-PA 1..118 1..120 470 71.7 Plus