Clone BS03453 Report

Search the DGRC for BS03453

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:34
Well:53
Vector:pDNR-Dual
Associated Gene/Transcriptarm-RA
Protein status:BS03453.pep: full length peptide match
Sequenced Size:2564

Clone Sequence Records

BS03453.3prime Sequence

768 bp (706 high quality bases) assembled on 2005-12-01

> BS03453.3prime
ATGGTCTAGAAAGCTTCTAACAATCGGTATCGTACCAGGCAGCCAGGTTG
TTGTTGTCATTGGGTGGCGTCGGCATGGCGTCCAAGTCAAAGTTCAATGC
ACCGGCATCAATCTCGCCAACGTCCATGTCCATGGAATAGGGCGAAGTTG
GTGCGCCGCTAGGCGGAATGGCGCCGATGTTACCACCGCCGCCGCTAGCT
CCGCCTACAGCTCCACCATTGCCGGGAGCACCGCCAGCACCGCCGCCGCC
CACTGGGCTGCTGATCTCCAGACCCTGCATCGAATCTATTGGTAGAGTAT
CATATCCTTGCTGATGGAATGCGCGACCTCCGTGTGAACTGTGCACGCTG
GGCGGACCTTGTCCGTACAGGCCCTCATATGCTTCTTCTGGTCCAAGCAT
ATCCTGTAGATCGGGACCCATGCCCAGGTCGGCATTGGCCCATATGTTGT
TGTCCTCGCGCAGCAGCGAGTTGGTCAGCTCTATGGATAGCCGCTTCTTG
TAATCCTGCGGCTTGTCCTCACTCATGCGAAAGAGAACAGCGGCGGCGTA
TGTGGCCACGCCTTCATTGCGCGAGTGCAGGAGATCGGTCAGCGGCCCAG
TGGCGCCCTCCTGCTCGATAATCTCGGCGCCCTCCTTGTCAGCGGCGAGC
TCACAAAGAACGCCAGCAGCCACGCGCTGTATGTTCTCGATTTCGTTGAA
CAGCAATCGTACAAAGATCGGTATTACCGACTGCTGGCGAATGAGTGCCC
GGTTGTGAGACTCCCGGG

BS03453.3prime Blast Records

Blast to dmel-all-CDS-r5.1.fasta performed 2007-05-06 16:31:58
Subject Length Description Subject Range Query Range Score Percent Strand
CG11579-PA 2532 arm-RA 1786..2532 763..17 3735 100 Minus
CG11579-PD 2532 arm-RD 1786..2532 763..17 3735 100 Minus
CG11579-PB 2532 arm-RB 1786..2532 763..17 3735 100 Minus
CG11579-PE 2532 arm-RE 1786..2532 763..17 3735 100 Minus
CG11579-PC 2166 arm-RC 1786..2151 763..398 1830 100 Minus
Blast to dmel-all-transcript-r6.02.fasta performed 2015-01-30 16:14:10
Subject Length Description Subject Range Query Range Score Percent Strand
arm-RF 3211 CG11579-RF 2031..2783 768..16 3750 99.9 Minus
arm-RE 3116 CG11579-RE 1936..2688 768..16 3750 99.9 Minus
arm-RB 3220 CG11579-RB 2040..2792 768..16 3750 99.9 Minus
Blast to na_all.dmel.RELEASE6 performed 2015-01-30 16:14:04
Subject Length Description Subject Range Query Range Score Percent Strand
X 23542271 X 1891829..1892121 16..308 1465 100 Plus
X 23542271 X 1894507..1894782 403..678 1380 100 Plus
X 23542271 X 1893402..1893503 306..407 510 100 Plus
X 23542271 X 1894841..1894932 677..768 445 98.9 Plus
Blast to na_te.dros performed on 2015-01-30 16:14:07 has no hits.

BS03453.3prime Sim4 Records

Sim4 to dmel-all-CDS-r4.3.fasta performed 2007-01-18 07:25:56 Download gff for BS03453.3prime
Subject Subject Range Query Range Percent Splice Strand
arm-PA 1781..2532 17..768 99   Minus
Sim4 to dmel-all-CDS-r5.1.fasta performed 2007-05-10 07:40:31 Download gff for BS03453.3prime
Subject Subject Range Query Range Percent Splice Strand
CG11579-PE 1781..2532 17..768 99   Minus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-05 11:22:59 Download gff for BS03453.3prime
Subject Subject Range Query Range Percent Splice Strand
arm-RB 2040..2792 16..768 99   Minus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2015-01-30 17:49:49 Download gff for BS03453.3prime
Subject Subject Range Query Range Percent Splice Strand
arm-RB 2040..2792 16..768 99   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2015-01-30 17:49:49 Download gff for BS03453.3prime
Subject Subject Range Query Range Percent Splice Strand
X 1893403..1893499 307..403 100 <- Plus
X 1891829..1892119 16..306 100 <- Plus
X 1894508..1894780 404..676 100 <- Plus
X 1894841..1894932 677..768 98   Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-05 11:22:59 Download gff for BS03453.3prime
Subject Subject Range Query Range Percent Splice Strand
arm_X 1785862..1786152 16..306 100 <- Plus
arm_X 1787436..1787532 307..403 100 <- Plus
arm_X 1788541..1788813 404..676 100 <- Plus
arm_X 1788874..1788965 677..768 98   Plus

BS03453.5prime Sequence

736 bp (620 high quality bases) assembled on 2005-11-30

> BS03453.5prime
GAAGTTATCAGTCGACATGAGTTACATGCCAGCCCAGAATCGAACCATGT
CGCATAATAATCAATACAATCCACCTGATCTGCCGCCGATGGTATCCGCC
AAGGAGCAGACCCTCATGTGGCAGCAGAATTCGTACTTGGGCGACTCCGG
CATCCACTCGGGTGCCGTGACCCAGGTTCCATCGCTGTCTGGCAAGGAGG
ACGAGGAGATGGAGGGAGATCCACTTATGTTCGACCTGGACACCGGTTTC
CCGCAGAATTTCACACAAGACCAAGTGGACGATATGAACCAGCAACTGAG
CCAGACACGCTCCCAACGTGTACGTGCTGCCATGTTTCCGGAAACCCTGG
AGGAGGGCATTGAGATTCCCTCCACCCAGTTTGATCCCCAACAGCCGACG
GCAGTGCAACGTCTTTCGGAACCGTCACAAATGCTAAAGCACGCGGTGGT
CAATCTGATCAACTACCAGGACGACGCTGAGCTGGCAACCAGGGCCATAC
CCGAGTTGATCAAGCTGCTGAACGATGAGGATCAGGTGGTAGTTTCCCAG
GCCGCCATGATGGTCCACCAGCTGTCTAAGAAGGAGGCCTCGCGACATGC
CATTATGAACAGCCCTCAGATGGGAGCCGCTTTGGTGCGTGCCATCTCTA
ACAGCAACGATCTGGAGAGCACCAAGGCAGCGGGAGGAACACTGCACAAC
TTATCACACCATCGCCAGGGTCTGCTGGGCATCTTC

BS03453.5prime Blast Records

Blast to dmel-all-CDS-r5.1.fasta performed 2007-05-06 16:31:59
Subject Length Description Subject Range Query Range Score Percent Strand
CG11579-PC 2166 arm-RC 1..720 17..736 3525 99.5 Plus
CG11579-PA 2532 arm-RA 1..720 17..736 3525 99.5 Plus
CG11579-PD 2532 arm-RD 1..720 17..736 3525 99.5 Plus
CG11579-PB 2532 arm-RB 1..720 17..736 3525 99.5 Plus
CG11579-PE 2532 arm-RE 1..720 17..736 3525 99.5 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2015-02-11 11:27:41
Subject Length Description Subject Range Query Range Score Percent Strand
arm-RF 3211 CG11579-RF 251..970 17..736 3555 99.6 Plus
arm-RE 3116 CG11579-RE 156..875 17..736 3555 99.6 Plus
arm-RB 3220 CG11579-RB 260..979 17..736 3555 99.6 Plus
Blast to na_all.dmel.RELEASE6 performed 2015-02-11 11:27:36
Subject Length Description Subject Range Query Range Score Percent Strand
X 23542271 X 1896065..1896753 736..48 3400 99.6 Minus
Blast to na_te.dros performed on 2015-02-11 11:27:38 has no hits.

BS03453.5prime Sim4 Records

Sim4 to dmel-all-CDS-r4.3.fasta performed 2007-01-18 07:25:57 Download gff for BS03453.5prime
Subject Subject Range Query Range Percent Splice Strand
arm-PA 1..720 17..736 99   Plus
Sim4 to dmel-all-CDS-r5.1.fasta performed 2007-05-10 07:40:33 Download gff for BS03453.5prime
Subject Subject Range Query Range Percent Splice Strand
CG11579-PE 1..720 17..736 99   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-24 15:35:12 Download gff for BS03453.5prime
Subject Subject Range Query Range Percent Splice Strand
arm-RB 251..979 7..736 99   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2015-02-11 12:46:36 Download gff for BS03453.5prime
Subject Subject Range Query Range Percent Splice Strand
arm-RB 251..979 7..736 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2015-02-11 12:46:36 Download gff for BS03453.5prime
Subject Subject Range Query Range Percent Splice Strand
X 1896065..1896753 48..736 99 <- Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-24 15:35:12 Download gff for BS03453.5prime
Subject Subject Range Query Range Percent Splice Strand
arm_X 1790098..1790786 48..736 99 <- Minus

BS03453.complete Sequence

2564 bp assembled on 2010-05-10

GenBank Submission: KX796692

> BS03453.complete
GAAGTTATCAGTCGACATGAGTTACATGCCAGCCCAGAATCGAACCATGT
CGCATAATAATCAATACAATCCACCTGATCTGCCGCCGATGGTATCCGCC
AAGGAGCAGACCCTCATGTGGCAGCAGAATTCGTACTTGGGCGACTCCGG
CATCCACTCGGGTGCCGTGACCCAGGTTCCATCGCTGTCTGGCAAGGAGG
ACGAGGAGATGGAGGGAGATCCACTTATGTTCGACCTGGACACCGGTTTC
CCGCAGAATTTCACACAAGACCAAGTGGACGATATGAACCAGCAACTGAG
CCAGACACGCTCCCAACGTGTACGTGCTGCCATGTTTCCGGAAACCCTGG
AGGAGGGCATTGAGATTCCCTCCACCCAGTTTGATCCCCAACAGCCGACG
GCAGTGCAACGTCTTTCGGAACCGTCACAAATGCTAAAGCACGCGGTGGT
CAATCTGATCAACTACCAGGACGACGCTGAGCTGGCAACCAGGGCCATAC
CCGAGTTGATCAAGCTGCTGAACGATGAGGATCAGGTGGTAGTTTCCCAG
GCCGCCATGATGGTCCACCAGCTGTCTAAGAAGGAGGCCTCGCGACATGC
CATTATGAACAGCCCTCAGATGGTAGCCGCTTTGGTGCGTGCCATCTCTA
ACAGCAACGATCTGGAGAGCACCAAGGCAGCGGTAGGAACACTGCACAAC
TTATCACACCATCGCCAGGGTCTGCTGGCCATCTTCAAGAGTGGCGGCAT
CCCGGCACTCGTCAAGTTGCTCTCCTCGCCAGTGGAGAGTGTGCTGTTCT
ATGCAATTACCACTCTGCACAATTTGCTGCTCCACCAGGATGGCTCTAAG
ATGGCTGTGCGCCTGGCCGGCGGGCTTCAGAAGATGGTTACTCTGCTGCA
ACGAAACAACGTTAAGTTTCTGGCTATCGTCACAGATTGCTTGCAAATTC
TGGCCTATGGTAACCAGGAGAGCAAGTTAATAATTCTTGCCTCCGGAGGG
CCCAACGAACTCGTGCGCATTATGCGCTCCTACGACTACGAGAAGCTTCT
GTGGACCACTTCGCGGGTACTGAAAGTGCTCTCCGTTTGCTCCAGCAACA
AGCCGGCCATCGTGGATGCCGGTGGAATGCAGGCGCTGGCTATGCACTTG
GGTAACATGTCACCGCGCCTTGTGCAAAACTGTTTGTGGACGCTCCGCAA
TCTTTCGGATGCAGCCACTAAGGTGGAGGGCCTTGAAGCTTTGCTCCAAT
CTCTCGTCCAGGTTCTGGGCTCGACCGATGTCAACGTGGTCACCTGTGCC
GCCGGTATCCTTTCAAATCTGACGTGCAACAATCAGCGCAACAAGGCCAC
CGTTTGTCAGGTGGGCGGTGTGGACGCCCTCGTCCGTACTATTATCAATG
CTGGAGATCGCGAAGAGATTACCGAGCCGGCTGTATGTGCCCTGCGTCAC
TTGACCTCGCGTCATGTGGACTCTGAGTTGGCCCAGAATGCCGTACGCTT
AAACTACGGACTATCGGTGATTGTAAAGCTATTGCATCCACCATCACGCT
GGCCCTTGATCAAGGCCGTCATTGGACTCATACGCAATTTGGCCCTCTGT
CCGGCCAATCACGCCCCGTTGCGGGAGCACGGGGCCATCCACCATCTGGT
GCGACTGCTTATGCGCGCCTTCCAAGACACAGAGAGGCAACGTTCCTCGA
TAGCCACCACTGGTTCACAGCAGCCGTCCGCATACGCTGACGGCGTTCGC
ATGGAGGAGATTGTCGAGGGCACGGTGGGGGCGCTACATATCCTGGCCCG
CGAGTCTCACAACCGGGCACTCATTCGCCAGCAGTCGGTAATACCGATCT
TTGTACGATTGCTGTTCAACGAAATCGAGAACATACAGCGCGTGGCTGCT
GGCGTTCTTTGTGAGCTCGCCGCTGACAAGGAGGGCGCCGAGATTATCGA
GCAGGAGGGCGCCACTGGGCCGCTGACCGATCTCCTGCACTCGCGCAATG
AAGGCGTGGCCACATACGCCGCCGCTGTTCTCTTTCGCATGAGTGAGGAC
AAGCCGCAGGATTACAAGAAGCGGCTATCCATAGAGCTGACCAACTCGCT
GCTGCGCGAGGACAACAACATATGGGCCAATGCCGACCTGGGCATGGGTC
CCGATCTACAGGATATGCTTGGACCAGAAGAAGCATATGAGGGCCTGTAC
GGACAAGGTCCGCCCAGCGTGCACAGTTCACACGGAGGTCGCGCATTCCA
TCAGCAAGGATATGATACTCTACCAATAGATTCGATGCAGGGTCTGGAGA
TCAGCAGCCCAGTGGGCGGCGGCGGTGCTGGCGGTGCTCCCGGCAATGGT
GGAGCTGTAGGCGGAGCTAGCGGCGGCGGTGGTAACATCGGCGCCATTCC
GCCTAGCGGCGCACCAACTTCGCCCTATTCCATGGACATGGACGTTGGCG
AGATTGATGCCGGTGCATTGAACTTTGACTTGGACGCCATGCCGACGCCA
CCCAATGACAACAACAACCTGGCTGCCTGGTACGATACCGATTGTTAGAA
GCTTTCTAGACCAT

BS03453.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-28 01:56:14
Subject Length Description Subject Range Query Range Score Percent Strand
arm-RF 2532 CG11579-PF 1..2532 17..2548 12660 100 Plus
arm-RE 2532 CG11579-PE 1..2532 17..2548 12660 100 Plus
arm-RB 2532 CG11579-PB 1..2532 17..2548 12660 100 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-28 01:56:15
Subject Length Description Subject Range Query Range Score Percent Strand
arm-RF 3211 CG11579-RF 251..2783 17..2549 12665 100 Plus
arm-RE 3116 CG11579-RE 156..2688 17..2549 12665 100 Plus
arm-RB 3220 CG11579-RB 260..2792 17..2549 12665 100 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-28 01:56:12
Subject Length Description Subject Range Query Range Score Percent Strand
X 23542271 X 1895114..1896753 1687..48 8200 100 Minus
X 23542271 X 1891829..1892121 2549..2257 1465 100 Minus
X 23542271 X 1894507..1894782 2162..1887 1380 100 Minus
X 23542271 X 1894841..1895042 1888..1687 1010 100 Minus
X 23542271 X 1893402..1893503 2259..2158 510 100 Minus
Blast to na_te.dros performed on 2014-11-28 01:56:13 has no hits.

BS03453.complete Sim4 Records

Sim4 to dmel-all-transcript-r5.12.fasta performed 2010-05-10 11:55:39 Download gff for BS03453.complete
Subject Subject Range Query Range Percent Splice Strand
arm-RB 260..2791 17..2548 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 21:06:18 Download gff for BS03453.complete
Subject Subject Range Query Range Percent Splice Strand
arm-RB 260..2791 17..2548 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-28 03:17:48 Download gff for BS03453.complete
Subject Subject Range Query Range Percent Splice Strand
arm-RB 260..2791 17..2548 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-28 03:17:48 Download gff for BS03453.complete
Subject Subject Range Query Range Percent Splice Strand
X 1894508..1894780 1889..2161 100 <- Minus
X 1894841..1895041 1688..1888 100 <- Minus
X 1891830..1892119 2259..2548 100 <- Minus
X 1893403..1893499 2162..2258 100 <- Minus
X 1895114..1896753 48..1687 100 <- Minus
X 1898980..1899010 17..47 100   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 21:06:18 Download gff for BS03453.complete
Subject Subject Range Query Range Percent Splice Strand
arm_X 1788541..1788813 1889..2161 100 <- Minus
arm_X 1788874..1789074 1688..1888 100 <- Minus
arm_X 1785863..1786152 2259..2548 100 <- Minus
arm_X 1787436..1787532 2162..2258 100 <- Minus
arm_X 1789147..1790786 48..1687 100 <- Minus
arm_X 1793013..1793043 17..47 100   Minus

BS03453.pep Sequence

Translation from 16 to 2547

> BS03453.pep
MSYMPAQNRTMSHNNQYNPPDLPPMVSAKEQTLMWQQNSYLGDSGIHSGA
VTQVPSLSGKEDEEMEGDPLMFDLDTGFPQNFTQDQVDDMNQQLSQTRSQ
RVRAAMFPETLEEGIEIPSTQFDPQQPTAVQRLSEPSQMLKHAVVNLINY
QDDAELATRAIPELIKLLNDEDQVVVSQAAMMVHQLSKKEASRHAIMNSP
QMVAALVRAISNSNDLESTKAAVGTLHNLSHHRQGLLAIFKSGGIPALVK
LLSSPVESVLFYAITTLHNLLLHQDGSKMAVRLAGGLQKMVTLLQRNNVK
FLAIVTDCLQILAYGNQESKLIILASGGPNELVRIMRSYDYEKLLWTTSR
VLKVLSVCSSNKPAIVDAGGMQALAMHLGNMSPRLVQNCLWTLRNLSDAA
TKVEGLEALLQSLVQVLGSTDVNVVTCAAGILSNLTCNNQRNKATVCQVG
GVDALVRTIINAGDREEITEPAVCALRHLTSRHVDSELAQNAVRLNYGLS
VIVKLLHPPSRWPLIKAVIGLIRNLALCPANHAPLREHGAIHHLVRLLMR
AFQDTERQRSSIATTGSQQPSAYADGVRMEEIVEGTVGALHILARESHNR
ALIRQQSVIPIFVRLLFNEIENIQRVAAGVLCELAADKEGAEIIEQEGAT
GPLTDLLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSIELTNSLLREDN
NIWANADLGMGPDLQDMLGPEEAYEGLYGQGPPSVHSSHGGRAFHQQGYD
TLPIDSMQGLEISSPVGGGGAGGAPGNGGAVGGASGGGGNIGAIPPSGAP
TSPYSMDMDVGEIDAGALNFDLDAMPTPPNDNNNLAAWYDTDC*

BS03453.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-29 02:18:10
Subject Length Description Subject Range Query Range Score Percent Strand
arm-PF 843 CG11579-PF 1..843 1..843 4318 100 Plus
arm-PE 843 CG11579-PE 1..843 1..843 4318 100 Plus
arm-PB 843 CG11579-PB 1..843 1..843 4318 100 Plus
arm-PD 843 CG11579-PD 1..843 1..843 4318 100 Plus
arm-PA 843 CG11579-PA 1..843 1..843 4318 100 Plus