Clone BS04404 Report

Search the DGRC for BS04404

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:44
Well:4
Vector:pDNR-Dual
Associated Gene/Transcriptrepo-RA
Protein status:BS04404.pep: full length peptide match
Sequenced Size:16

Clone Sequence Records

BS04404.3prime Sequence

934 bp (826 high quality bases) assembled on 2005-12-01

> BS04404.3prime
ATGGTCTAGAAAGCTTTCAGTGCAAAAAAGGCGGCAGGACGTAGCTCTGT
CCCTGCTGCTGCTGCTGCTGTTGGGAGACCTCCTCGCTCTTGATCACAGT
CTGCATGCCGCCGCTGTCGTTGTCATCGCTGCTGGTGCCGCCCGCGGATG
CAGCTCCTCCCGTCGCCGTGGTCACATAGTGCTGGCCCTGCGCCTCCACC
AGATGACAGCTGGCGCCGGGACTTAGCTCCAGACCACCTCCACTCTGATG
GCACTGCAGGTGCACGTATTTGGCCTCCTCGGCGCTCAGGTACTTGCCAT
TGAGTTGCTGCTGCTGCTGCTGCTGCTGTTGGTGGGCATGGACGGTGACC
AGTGTGCCGTCGGCCAGTTGCTGCTGCTGGGCGGTCTGCAGTTCCGCCGT
CTGATAGCTGCCGCCATTGGCCACCGACTCATCTCCGTTTCCGGCCACCG
AACCGGTTGTGGCGCCCATTTCCATGCGCGTGGGACTACCGTACTCGTAG
TGACGCATCGGGAAGAGCTGGCTTTCGTGCACATAGGCACCGTACTGGCC
GGAATAGGTGCCATACGGACTGGCCGCCGGCGAGAGCAGGCTGAACTGGG
GCGTCAGTTCGTAGGGCGTCTGGTAGCTGGTCCAGCTGTCCATGCTGCCC
GGCGGCGTGACCGTCGTCGTCTGCGGGTAGCTAGTGAATGGTGGGGCTAG
GGTTCCGGGATTCAGTGTCGCCGTCGGCGGAGTGCTCGTCTTGATGTAGC
CCGTCTTGCGCGGTGGCTCGTGCTTGCGCCACTTGGCCCGTCGGTTCTGG
AACCACACCTGCACACGGGATTCGCTCAGATTCAGCTTGATGGCCAGCTC
CTCGCGGGCGAAAACATCCGGATACGGAGCACGTTCAAAGGCACGCTCCA
ACTCCTCCAGCTGGAAGGCAGTAAAGGTGGTTCT

BS04404.3prime Blast Records

Blast to dmel-all-CDS-r5.1.fasta performed 2007-05-06 16:47:16
Subject Length Description Subject Range Query Range Score Percent Strand
CG31240-PA 1839 repo-RA 922..1839 934..17 4565 99.8 Minus
CG2819-PA 1074 Pph13-RA 160..203 814..771 195 97.7 Minus
CG11294-PA 786 CG11294-RA 205..245 811..771 155 95.1 Minus
CG2851-PA 1260 Gsc-RA 992..1031 810..771 150 95 Minus
CG33980-PA 1923 CG33980-RA 823..861 811..773 145 94.8 Minus
Blast to dmel-all-transcript-r6.02.fasta performed 2015-02-10 22:38:09
Subject Length Description Subject Range Query Range Score Percent Strand
repo-RA 2996 CG31240-RA 1227..2144 934..17 4575 99.9 Minus
CG34340-RF 2989 CG34340-RF 776..916 913..773 255 79.6 Minus
CG34340-RE 2612 CG34340-RE 776..916 913..773 255 79.6 Minus
Blast to na_all.dmel.RELEASE6 performed 2015-02-10 22:38:02
Subject Length Description Subject Range Query Range Score Percent Strand
3R 32079331 3R 18237833..18238750 934..17 4575 99.9 Minus
2L 23513712 2L 578273..578320 814..767 210 95.8 Minus
X 23542271 X 8692709..8692751 771..813 185 95.3 Plus
Blast to na_te.dros performed 2015-02-10 22:38:06
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6791..6821 334..304 137 93.5 Minus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2280..2432 458..305 118 59.1 Minus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6806..6917 419..304 110 62.2 Minus

BS04404.3prime Sim4 Records

Sim4 to dmel-all-CDS-r4.3.fasta performed 2007-01-18 07:47:25 Download gff for BS04404.3prime
Subject Subject Range Query Range Percent Splice Strand
repo-PA 922..1839 17..934 99   Minus
Sim4 to dmel-all-CDS-r5.1.fasta performed 2007-05-10 08:08:33 Download gff for BS04404.3prime
Subject Subject Range Query Range Percent Splice Strand
CG31240-PA 922..1839 17..934 99   Minus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-21 09:27:57 Download gff for BS04404.3prime
Subject Subject Range Query Range Percent Splice Strand
repo-RA 1225..2145 14..934 99   Minus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2015-02-11 00:04:00 Download gff for BS04404.3prime
Subject Subject Range Query Range Percent Splice Strand
repo-RA 1227..2147 14..934 99   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2015-02-11 00:04:00 Download gff for BS04404.3prime
Subject Subject Range Query Range Percent Splice Strand
3R 18237833..18238748 19..934 99 -> Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-21 09:27:57 Download gff for BS04404.3prime
Subject Subject Range Query Range Percent Splice Strand
arm_3R 14063555..14064470 19..934 99 -> Minus

BS04404.5prime Sequence

870 bp (798 high quality bases) assembled on 2005-12-01

> BS04404.5prime
GAAGTTATCAGTCGACATGGAACACGATAGCTTCGATGATCCCATTTTCG
GGGAGTTCGGCGGCGGGCCACTCAATCCACTGGGCGCCAAGCCACTGATG
CCCACCACAACGGCCATGCATCCCGTGATGCTGGGCAGTGTCCACGAGTT
GTGCAGCCAACAGCAGCAGCAGCAGCAGCAGCAACGATTGCCCGACTGCA
ACACCATACTGCCGAATGGCGGAGGAGGAGGAGCAGGATCTGGCGGCGCT
GGAGGATCGCCCAACTATGTGACCAAGCTGGATTTTGTCAACAAGATGGG
CTGCTACTCGCCCAGCCAGAAGTACGAGTACATTAGTGCGCCGCAAAAAC
TGGTGGAGCAGCAGCAGCAGCAGCAGCATCATCATCAACATCATCAGCAC
TACACGGCCACGCCCCCGCCCAGCCACAATGGCATCCTGCTGCACAAGCA
ATCCTCCCAGCAGCAACAGCAGCAGCAGCAACAGCTGGTGGGCATCCTGG
ACTATCATCCGCTCAATGGCAAGCTGGACTACTCCTCGTCGCCCAAAGAT
CAGTACGAGCCGCAGCAGCTGCAGCATCTGTACGGCGGCAGTCCGCATCA
TCTGGACCATTTGGATCATGGCAGTGATGGCTTGCTCCAGGACTCCTCGC
CCGTCATGATCAACGGAGGATCCGCTGGTGGCAAGCTAAAGAAGCCCGAT
GAGATGTGTTCGCAGTTGGAGGCGGGTGGTGCTGGAGTTACTCCGCCAAG
TAGTTCCTCCACGGTGGTTAATGGCACCACCAATGGCACTGGCAACGCCA
ACTCCTCCTCCTCCGCTGGCGTCGGCATTCAGGGGGCGGCGGGCACAGCG
GGGGGGCGTGGCAGCCTCTG

BS04404.5prime Blast Records

Blast to dmel-all-CDS-r5.1.fasta performed 2007-05-06 16:47:17
Subject Length Description Subject Range Query Range Score Percent Strand
CG31240-PA 1839 repo-RA 1..839 17..855 4195 100 Plus
CG15771-PA 1068 CG15771-RA 944..978 363..397 150 97.1 Plus
CG18599-PA 1428 CG18599-RA 252..293 358..399 135 92.8 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2015-02-12 05:38:37
Subject Length Description Subject Range Query Range Score Percent Strand
repo-RA 2996 CG31240-RA 306..1153 17..865 4220 99.9 Plus
Blast to na_all.dmel.RELEASE6 performed 2015-02-12 05:38:29
Subject Length Description Subject Range Query Range Score Percent Strand
3R 32079331 3R 18236497..18237335 17..855 4195 100 Plus
Blast to na_te.dros performed on 2015-02-12 05:38:33 has no hits.

BS04404.5prime Sim4 Records

Sim4 to dmel-all-CDS-r4.3.fasta performed 2007-01-18 07:47:26 Download gff for BS04404.5prime
Subject Subject Range Query Range Percent Splice Strand
repo-PA 1..853 17..870 99   Plus
Sim4 to dmel-all-CDS-r5.1.fasta performed 2007-05-10 08:08:35 Download gff for BS04404.5prime
Subject Subject Range Query Range Percent Splice Strand
CG31240-PA 1..853 17..870 99   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-29 03:20:27 Download gff for BS04404.5prime
Subject Subject Range Query Range Percent Splice Strand
repo-RA 298..1156 10..870 99   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2015-02-12 07:45:36 Download gff for BS04404.5prime
Subject Subject Range Query Range Percent Splice Strand
repo-RA 300..1158 10..870 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2015-02-12 07:45:36 Download gff for BS04404.5prime
Subject Subject Range Query Range Percent Splice Strand
3R 18236491..18237349 10..870 99   Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-29 03:20:27 Download gff for BS04404.5prime
Subject Subject Range Query Range Percent Splice Strand
arm_3R 14062213..14063071 10..870 99   Plus

BS04404.complete Sequence

1871 bp assembled on 2008-01-02

GenBank Submission: KX796800

> BS04404.complete
GAAGTTATCAGTCGACATGGAACACGATAGCTTCGATGATCCCATTTTCG
GGGAGTTCGGCGGCGGGCCACTCAATCCACTGGGCGCCAAGCCACTGATG
CCCACCACAACGGCCATGCATCCCGTGATGCTGGGCAGTGTCCACGAGTT
GTGCAGCCAACAGCAGCAGCAGCAGCAGCAGCAACGATTGCCCGACTGCA
ACACCATACTGCCGAATGGCGGAGGAGGAGGAGCAGGATCTGGCGGCGCT
GGAGGATCGCCCAACTATGTGACCAAGCTGGATTTTGTCAACAAGATGGG
CTGCTACTCGCCCAGCCAGAAGTACGAGTACATTAGTGCGCCGCAAAAAC
TGGTGGAGCAGCAGCAGCAGCAGCAGCATCATCATCAACATCATCAGCAC
TACACGGCCACGCCCCCGCCCAGCCACAATGGCATCCTGCTGCACAAGCA
ATCCTCCCAGCAGCAACAGCAGCAGCAGCAACAGCTGGTGGGCATCCTGG
ACTATCATCCGCTCAATGGCAAGCTGGACTACTCCTCGTCGCCCAAAGAT
CAGTACGAGCCGCAGCAGCTGCAGCATCTGTACGGCGGCAGTCCGCATCA
TCTGGACCATTTGGATCATGGCAGTGATGGCTTGCTCCAGGACTCCTCGC
CCGTCATGATCAACGGAGGATCCGCTGGTGGCAAGCTAAAGAAGCCCGAT
GAGATGTGTTCGCAGTTGGAGGCGGGTGGTGCTGGAGTTACTCCGCCAAG
TAGTTCCTCCACGGTGGTTAATGGCACCACCAATGGCACTGGCAACGCCA
ACTCCTCCTCCTCCGCTGGCGTCGGCATTCAGGGGGCGGCGGGCACAGCG
GGGGGCGTGGCAGCTCCTGCAGCCAAAAAGGACGGAAGCAGCAGCAAGAA
GAAGGGCGATCCCAATGGCATCAAGAAGAAGAAGACGAGAACCACCTTTA
CTGCCTACCAGCTGGAGGAGTTGGAGCGTGCCTTTGAACGTGCTCCGTAT
CCGGATGTTTTCGCCCGCGAGGAGCTGGCCATCAAGCTGAATCTGAGCGA
ATCCCGTGTGCAGGTGTGGTTCCAGAACCGACGGGCCAAGTGGCGCAAGC
ACGAGCCACCGCGCAAGACGGGCTACATCAAGACGAGCACTCCGCCGACG
GCGACACTGAATCCCGGAACCCTAGCCCCACCATTCACTAGCTACCCGCA
GACGACGACGGTCACGCCGCCGGGCAGCATGGACAGCTGGACCAGCTACC
AGACGCCCTACGAACTGACGCCCCAGTTCAGCCTGCTCTCGCCGGCGGCC
AGTCCGTATGGCACCTATTCCGGCCAGTACGGTGCCTATGTGCACGAAAG
CCAGCTCTTCCCGATGCGTCACTACGAGTACGGTAGTCCCACGCGCATGG
AAATGGGCGCCACAACCGGTTCGGTGGCCGGAAACGGAGATGAGTCGGTG
GCCAATGGCGGCAGCTATCAGACGGCGGAACTGCAGACCGCCCAGCAGCA
GCAACTGGCCGACGGCACACTGGTCACCGTCCATGCCCACCAACAGCAGC
AGCAGCAGCAGCAGCAACTCAATGGCAAGTACCTGAGCGCCGAGGAGGCC
AAATACGTGCACCTGCAGTGCCATCAGAGTGGAGGTGGTCTGGAGCTAAG
TCCCGGCGCCAGCTGTCATCTGGTGGAGGCGCAGGGCCAGCACTATGTGA
CCACGGCGACGGGAGGAGCTGCATCCGCGGGCGGCACCAGCAGCGATGAC
AACGACAGCGGCGGCATGCAGACTGTGATCAAGAGCGAGGAGGTCTCCCA
ACAGCAGCAGCAGCAGCAGGGACAGAGCTACGTCCTGCCGCCTTTTTTGC
ACTGAAAGCTTTCTAGACCAT

BS04404.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-27 17:04:18
Subject Length Description Subject Range Query Range Score Percent Strand
repo-RA 1839 CG31240-PA 1..1839 17..1855 9195 100 Plus
CG34340-RF 1764 CG34340-PF 187..327 959..1099 255 78.7 Plus
CG34340-RE 1764 CG34340-PE 187..327 959..1099 255 78.7 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-27 17:04:19
Subject Length Description Subject Range Query Range Score Percent Strand
repo-RA 2996 CG31240-RA 306..2144 17..1855 9195 100 Plus
CG34340-RF 2989 CG34340-RF 776..916 959..1099 255 78.7 Plus
CG34340-RE 2612 CG34340-RE 776..916 959..1099 255 78.7 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-27 17:04:16
Subject Length Description Subject Range Query Range Score Percent Strand
3R 32079331 3R 18236497..18237419 17..939 4615 100 Plus
3R 32079331 3R 18237833..18238750 938..1855 4590 100 Plus
2L 23513712 2L 578273..578320 1058..1105 210 95.8 Plus
X 23542271 X 8692709..8692751 1101..1059 185 95.3 Minus
Blast to na_te.dros performed 2014-11-27 17:04:17
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2280..2432 1414..1567 118 59.1 Plus

BS04404.complete Sim4 Records

Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-21 23:05:00 Download gff for BS04404.complete
Subject Subject Range Query Range Percent Splice Strand
repo-RA 1..1839 17..1855 100   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2009-07-28 15:35:12 Download gff for BS04404.complete
Subject Subject Range Query Range Percent Splice Strand
repo-RA 304..2141 17..1854 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 19:02:59 Download gff for BS04404.complete
Subject Subject Range Query Range Percent Splice Strand
repo-RA 304..2141 17..1854 100   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-21 23:05:01 Download gff for BS04404.complete
Subject Subject Range Query Range Percent Splice Strand
repo-RA 298..2145 10..1858 99   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-27 17:33:57 Download gff for BS04404.complete
Subject Subject Range Query Range Percent Splice Strand
repo-RA 306..2143 17..1854 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-27 17:33:57 Download gff for BS04404.complete
Subject Subject Range Query Range Percent Splice Strand
3R 18236497..18237419 17..939 100 -> Plus
3R 18237835..18238749 940..1854 100   Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 19:02:59 Download gff for BS04404.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3R 14062219..14063141 17..939 100 -> Plus
arm_3R 14063557..14064471 940..1854 100   Plus

BS04404.pep Sequence

Translation from 16 to 1854

> BS04404.pep
MEHDSFDDPIFGEFGGGPLNPLGAKPLMPTTTAMHPVMLGSVHELCSQQQ
QQQQQQRLPDCNTILPNGGGGGAGSGGAGGSPNYVTKLDFVNKMGCYSPS
QKYEYISAPQKLVEQQQQQQHHHQHHQHYTATPPPSHNGILLHKQSSQQQ
QQQQQQLVGILDYHPLNGKLDYSSSPKDQYEPQQLQHLYGGSPHHLDHLD
HGSDGLLQDSSPVMINGGSAGGKLKKPDEMCSQLEAGGAGVTPPSSSSTV
VNGTTNGTGNANSSSSAGVGIQGAAGTAGGVAAPAAKKDGSSSKKKGDPN
GIKKKKTRTTFTAYQLEELERAFERAPYPDVFAREELAIKLNLSESRVQV
WFQNRRAKWRKHEPPRKTGYIKTSTPPTATLNPGTLAPPFTSYPQTTTVT
PPGSMDSWTSYQTPYELTPQFSLLSPAASPYGTYSGQYGAYVHESQLFPM
RHYEYGSPTRMEMGATTGSVAGNGDESVANGGSYQTAELQTAQQQQLADG
TLVTVHAHQQQQQQQQQLNGKYLSAEEAKYVHLQCHQSGGGLELSPGASC
HLVEAQGQHYVTTATGGAASAGGTSSDDNDSGGMQTVIKSEEVSQQQQQQ
QGQSYVLPPFLH*

BS04404.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 18:40:15
Subject Length Description Subject Range Query Range Score Percent Strand
repo-PA 612 CG31240-PA 1..612 1..612 3264 100 Plus
Rx-PB 904 CG10052-PB 450..679 113..405 294 32.5 Plus
al-PA 408 CG3935-PA 17..144 223..363 274 43.3 Plus
prd-PA 590 CG6716-PA 133..455 243..593 256 27.5 Plus
prd-PB 613 CG6716-PB 156..478 243..593 256 27.5 Plus