Clone BS05025 Report

Search the DGRC for BS05025

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:50
Well:25
Vector:pDNR-Dual
Associated Gene/Transcriptsky-RA
Protein status:BS05025.pep: full length peptide match
Sequenced Size:16

Clone Sequence Records

BS05025.3prime Sequence

911 bp (874 high quality bases) assembled on 2005-12-02

> BS05025.3prime
ATGGTCTAGAAAGCTTTTAGATGCCCACGAATCCGTAGACTTCCAGCACC
CGAATCTCGAAGTCGCCCGACGGACACAGCGGCGGATTGTTAAAGGTCTT
GCAGCTGTCCGTCTTTCCGAAACGAATGTTCTCGTCCATCCAGATGGCCT
GCCCCTCGCCGCCACCAATAGTGATCATCTTGGAGTCGGCCGCCATAAAT
AGTTCGGAGCTGTGTCCCAGGTCCTTGTCGCCCTCAATGCCCACCCATGG
GTACTTGGCTCTTTCTGGATATAGGGAGAACAAAAAGGTTTCGCCCGTAC
CGAAGTAGGCCTGTCGCTGGCCCTTGTCATCTTTTACATTTCTTTCAAAC
CAGCGCGAAGAGCAGTAGGCACCAAATACCTCATTATTGCACGTCTTGAT
CATGAGCAGCGTGGGTTCATGCTGCTCCACCCGAACGTAGAACGTGGTTA
GTGAGCAGCCGTGCTCCTCCGTGGTGTAGAGCAGCACCGGTTGGTACATT
GTTATCCGCACGGGCAGCCAGGACCACAGCGTGAACAGAAATTGTCTATC
CGTCCAGGTTTTTATGAAATTCTTTGTGCCCGGCAAACCAAAATGCTCTC
GGATGGTCAGGGTGTGGGACATCATTTGGATGTTGACCTGCGACTGACTA
GTGGGCAGATTATCACTTGAACGCGATTTGATTAATTGCTTCGAACCGCT
GGTGAGCACGGAGCGGCTTTTTAGTAGCATTTCAGTCTTGATAAATATTC
TAGAAATATACTGGGTACTCAGTCCCCTAATACTAAACGCGGCATGGAGC
AACTTCGCCGGCGACACTGGTATCTTCTTGCAGAACTTGATCAAAGCGTT
GCCAATGTCGTTTTTAATGTTGTCCGGACTCCATTCGTTGTTCGACTGGC
ACTCCTTGTGA

BS05025.3prime Blast Records

Blast to dmel-all-CDS-r5.1.fasta performed 2007-05-06 16:56:36
Subject Length Description Subject Range Query Range Score Percent Strand
CG9339-PB 1764 CG9339-RB 870..1764 911..17 4475 100 Minus
CG9339-PC 1779 CG9339-RC 885..1779 911..17 4475 100 Minus
CG9339-PH 1764 CG9339-RH 870..1764 911..17 4475 100 Minus
CG9339-PA 1764 CG9339-RA 870..1764 911..17 4475 100 Minus
CG9339-PE 1707 CG9339-RE 1186..1707 538..17 2610 100 Minus
CG9339-PF 1722 CG9339-RF 1201..1722 538..17 2610 100 Minus
CG9339-PD 1707 CG9339-RD 1186..1707 538..17 2610 100 Minus
CG9339-PG 1707 CG9339-RG 1186..1707 538..17 2610 100 Minus
CG9339-PE 1707 CG9339-RE 870..1186 911..595 1585 100 Minus
CG9339-PF 1722 CG9339-RF 885..1201 911..595 1585 100 Minus
CG9339-PD 1707 CG9339-RD 870..1186 911..595 1585 100 Minus
CG9339-PG 1707 CG9339-RG 870..1186 911..595 1585 100 Minus
Blast to dmel-all-transcript-r6.02.fasta performed 2015-02-02 19:03:36
Subject Length Description Subject Range Query Range Score Percent Strand
sky-RA 4920 CG9339-RA 1612..2515 911..8 4490 99.8 Minus
sky-RH 5080 CG9339-RH 1772..2675 911..8 4490 99.8 Minus
sky-RC 4758 CG9339-RC 1450..2353 911..8 4490 99.8 Minus
Blast to na_all.dmel.RELEASE6 performed 2015-02-02 19:03:29
Subject Length Description Subject Range Query Range Score Percent Strand
2L 23513712 2L 20873957..20874179 159..381 1115 100 Plus
2L 23513712 2L 20876912..20877085 596..769 870 100 Plus
2L 23513712 2L 20874271..20874432 377..538 810 100 Plus
2L 23513712 2L 20873740..20873891 8..159 730 98.7 Plus
2L 23513712 2L 20877149..20877292 768..911 720 100 Plus
2L 23513712 2L 20875080..20875138 539..597 295 100 Plus
Blast to na_te.dros performed on 2015-02-02 19:03:32 has no hits.

BS05025.3prime Sim4 Records

Sim4 to dmel-all-CDS-r4.3.fasta performed 2007-01-18 07:59:53 Download gff for BS05025.3prime
Subject Subject Range Query Range Percent Splice Strand
CG9339-PA 871..1764 17..910 100   Minus
Sim4 to dmel-all-CDS-r5.1.fasta performed 2007-05-10 08:24:51 Download gff for BS05025.3prime
Subject Subject Range Query Range Percent Splice Strand
CG9339-PA 871..1764 17..910 100   Minus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-08 18:14:12 Download gff for BS05025.3prime
Subject Subject Range Query Range Percent Splice Strand
sky-RC 1451..2353 8..910 99   Minus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2015-02-02 20:56:49 Download gff for BS05025.3prime
Subject Subject Range Query Range Percent Splice Strand
sky-RC 1451..2353 8..910 99   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2015-02-02 20:56:49 Download gff for BS05025.3prime
Subject Subject Range Query Range Percent Splice Strand
2L 20875080..20875136 539..595 100 <- Plus
2L 20876912..20877083 596..767 100 <- Plus
2L 20877149..20877291 768..910 100   Plus
2L 20874274..20874432 380..538 100 <- Plus
2L 20873740..20873890 8..158 98 <- Plus
2L 20873957..20874177 159..379 100 <- Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-08 18:14:12 Download gff for BS05025.3prime
Subject Subject Range Query Range Percent Splice Strand
arm_2L 20873740..20873890 8..158 98 <- Plus
arm_2L 20873957..20874177 159..379 100 <- Plus
arm_2L 20874274..20874432 380..538 100 <- Plus
arm_2L 20875080..20875136 539..595 100 <- Plus
arm_2L 20876912..20877083 596..767 100 <- Plus
arm_2L 20877149..20877291 768..910 100   Plus

BS05025.5prime Sequence

911 bp (842 high quality bases) assembled on 2005-12-02

> BS05025.5prime
GAAGTTATCAGTCGACATGCCATACCATCGAGGCGGAGATGCCTCTTCCC
AGGCCGACAAGCTGAGCGGCATCGTGGAGGAGAGCGATCTGTACGAAGGG
TTCGCCCCGCATGTCGAGACGTCCGAGATCAAAACCCTCGACTTCTACAA
TTTACCAAAGCAAACTGGCAAGGAACCGGCACTGCGCTCCTACACGGAAA
TCCAGCAGCTGCTGCAGCAGGGCAAGAAGCGCGACGTGAAGAACATACTC
AGGGAGAACTCGTGGCCCATTAACTCCCCGATCCGAGCCCAGCTATGGCC
GATGTTGTGTGGCCAGCACCAGACCAAACAGCAAATGCTAGATGGCTTCT
ACTGGGAGATGGTTCACCAGGTCTTTGGCACCACGGAGCTGTCGGAGAAG
CCGATCATGCTGCCCGCCTTTGTGGATGCCACCCACTGTTTGCCCTACCA
CTTGACCAGCACGGGACGAGCCGTGGCCGATCGCATCGTGAATGTCCTGG
GCTACGACTGCCCCGATATTACCTACAGTCCAGTATTGTATCCCATTACA
TCGATACTTTTGCATTTCATGTCGGAGGAGGAGGCGTACATATGTCTGGC
CGGTCTGGTCGGTAGCAAGGAGAAGGTATTCATCAATCAAACCAAACTAC
AGCACGAAGTCACTTGGAAGACGGTGATGCAGATAGCCAAAAAGCACACA
AAAAGTGCTACTTCGTATTTCCAACGTATTTGCCCGGGCCTGAAGCTAGA
ACGCATCTTCATGGACTGGTGCTGGTGGATTCTAGCCGGTCTACCCTTCC
AGCATCTGGTGCGGATCATGGACTGCTACTTCCACGAGGGCATCAAGGTC
CTGTACCGCGTGGCCCTCGTCATACTCAACCTGTTTCACAGGAGTGCCAG
TCGAACAACGA

BS05025.5prime Blast Records

Blast to dmel-all-CDS-r5.1.fasta performed 2007-05-06 16:56:36
Subject Length Description Subject Range Query Range Score Percent Strand
CG9339-PB 1764 CG9339-RB 1..874 17..890 4370 100 Plus
CG9339-PE 1707 CG9339-RE 1..874 17..890 4370 100 Plus
CG9339-PD 1707 CG9339-RD 1..874 17..890 4370 100 Plus
CG9339-PH 1764 CG9339-RH 1..874 17..890 4370 100 Plus
CG9339-PG 1707 CG9339-RG 1..874 17..890 4370 100 Plus
CG9339-PA 1764 CG9339-RA 1..874 17..890 4370 100 Plus
CG9339-PC 1779 CG9339-RC 166..889 167..890 3620 100 Plus
CG9339-PF 1722 CG9339-RF 166..889 167..890 3620 100 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2015-02-12 01:19:55
Subject Length Description Subject Range Query Range Score Percent Strand
CG43739-RA 4863 CG43739-RA 743..1638 17..911 4455 99.9 Plus
sky-RL 4921 CG9339-RL 687..1582 17..911 4455 99.9 Plus
sky-RK 5158 CG9339-RK 951..1846 17..911 4455 99.9 Plus
Blast to na_all.dmel.RELEASE6 performed 2015-02-12 01:19:50
Subject Length Description Subject Range Query Range Score Percent Strand
2L 23513712 2L 20881987..20882194 575..368 1040 100 Minus
2L 23513712 2L 20882260..20882465 372..167 1030 100 Minus
2L 23513712 2L 20877266..20877477 911..701 1010 99.5 Minus
2L 23513712 2L 20903015..20903166 168..17 760 100 Minus
2L 23513712 2L 20877553..20877678 700..575 630 100 Minus
Blast to na_te.dros performed on 2015-02-12 01:19:53 has no hits.

BS05025.5prime Sim4 Records

Sim4 to dmel-all-CDS-r4.3.fasta performed 2007-01-18 07:59:54 Download gff for BS05025.5prime
Subject Subject Range Query Range Percent Splice Strand
CG9339-PH 1..895 17..910 99   Plus
Sim4 to dmel-all-CDS-r5.1.fasta performed 2007-05-10 08:24:52 Download gff for BS05025.5prime
Subject Subject Range Query Range Percent Splice Strand
CG9339-PE 1..895 17..910 99   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-28 20:58:21 Download gff for BS05025.5prime
Subject Subject Range Query Range Percent Splice Strand
sky-RJ 760..1659 10..910 99   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2015-02-12 05:32:12 Download gff for BS05025.5prime
Subject Subject Range Query Range Percent Splice Strand
sky-RK 946..1845 10..910 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2015-02-12 05:32:12 Download gff for BS05025.5prime
Subject Subject Range Query Range Percent Splice Strand
2L 20877553..20877677 576..700 100 <- Minus
2L 20877267..20877477 701..910 99 <- Minus
2L 20881987..20882191 371..575 100 <- Minus
2L 20882262..20882464 168..370 100 <- Minus
2L 20903016..20903171 10..167 97   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-28 20:58:21 Download gff for BS05025.5prime
Subject Subject Range Query Range Percent Splice Strand
arm_2L 20877267..20877477 701..910 99 <- Minus
arm_2L 20877553..20877677 576..700 100 <- Minus
arm_2L 20881987..20882191 371..575 100 <- Minus
arm_2L 20882262..20882464 168..370 100 <- Minus
arm_2L 20903016..20903171 10..167 97   Minus

BS05025.complete Sequence

1796 bp assembled on 2007-10-26

GenBank Submission: FJ635040

> BS05025.complete
GAAGTTATCAGTCGACATGCCATACCATCGAGGCGGAGATGCCTCTTCCC
AGGCCGACAAGCTGAGCGGCATCGTGGAGGAGAGCGATCTGTACGAAGGG
TTCGCCCCGCATGTCGAGACGTCCGAGATCAAAACCCTCGACTTCTACAA
TTTACCAAAGCAAACTGGCAAGGAACCGGCACTGCGCTCCTACACGGAAA
TCCAGCAGCTGCTGCAGCAGGGCAAGAAGCGCGACGTGAAGAACATACTC
AGGGAGAACTCGTGGCCCATTAACTCCCCGATCCGAGCCCAGCTATGGCC
GATGTTGTGTGGCCAGCACCAGACCAAACAGCAAATGCTAGATGGCTTCT
ACTGGGAGATGGTTCACCAGGTCTTTGGCACCACGGAGCTGTCGGAGAAG
CCGATCATGCTGCCCGCCTTTGTGGATGCCACCCACTGTTTGCCCTACCA
CTTGACCAGCACGGGACGAGCCGTGGCCGATCGCATCGTGAATGTCCTGG
GCTACGACTGCCCCGATATTACCTACAGTCCAGTATTGTATCCCATTACA
TCGATACTTTTGCATTTCATGTCGGAGGAGGAGGCGTACATATGTCTGGC
CGGTCTGGTCGGTAGCAAGGAGAAGGTATTCATCAATCAAACCAAACTAC
AGCACGAAGTCACTTGGAAGACGGTGATGCAGATAGCCAAAAAGCACACA
AAAAGTGCTACTTCGTATTTCCAACGTATTTGCCCGGGCCTGAAGCTAGA
ACGCATCTTCATGGACTGGTGCTGGTGGATTCTAGCCGGTCTACCCTTCC
AGCATCTGGTGCGGATCATGGACTGCTACTTCCACGAGGGCATCAAGGTC
CTGTACCGCGTGGCCCTCGTCATACTCAACCTGTTTCACAAGGAGTGCCA
GTCGAACAACGAATGGAGTCCGGACAACATTAAAAACGACATTGGCAACG
CTTTGATCAAGTTCTGCAAGAAGATACCAGTGTCGCCGGCGAAGTTGCTC
CATGCCGCGTTTAGTATTAGGGGACTGAGTACCCAGTATATTTCTAGAAT
ATTTATCAAGACTGAAATGCTACTAAAAAGCCGCTCCGTGCTCACCAGCG
GTTCGAAGCAATTAATCAAATCGCGTTCAAGTGATAATCTGCCCACTAGT
CAGTCGCAGGTCAACATCCAAATGATGTCCCACACCCTGACCATCCGAGA
GCATTTTGGTTTGCCGGGCACAAAGAATTTCATAAAAACCTGGACGGATA
GACAATTTCTGTTCACGCTGTGGTCCTGGCTGCCCGTGCGGATAACAATG
TACCAACCGGTGCTGCTCTACACCACGGAGGAGCACGGCTGCTCACTAAC
CACGTTCTACGTTCGGGTGGAGCAGCATGAACCCACGCTGCTCATGATCA
AGACGTGCAATAATGAGGTATTTGGTGCCTACTGCTCTTCGCGCTGGTTT
GAAAGAAATGTAAAAGATGACAAGGGCCAGCGACAGGCCTACTTCGGTAC
GGGCGAAACCTTTTTGTTCTCCCTATATCCAGAAAGAGCCAAGTACCCAT
GGGTGGGCATTGAGGGCGACAAGGACCTGGGACACAGCTCCGAACTATTT
ATGGCGGCCGACTCCAAGATGATCACTATTGGTGGCGGCGAGGGGCAGGC
CATCTGGATGGACGAGAACATTCGTTTCGGAAAGACGGACAGCTGCAAGA
CCTTTAACAATCCGCCGCTGTGTCCGTCGGGCGACTTCGAGATTCGGGTG
CTGGAAGTCTACGGATTCGTGGGCATCTAAAAGCTTTCTAGACCAT

BS05025.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-28 05:44:38
Subject Length Description Subject Range Query Range Score Percent Strand
sky-RA 1764 CG9339-PA 1..1764 17..1780 8820 100 Plus
sky-RH 1764 CG9339-PH 1..1764 17..1780 8820 100 Plus
sky-RB 1764 CG9339-PB 1..1764 17..1780 8820 100 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-28 05:44:39
Subject Length Description Subject Range Query Range Score Percent Strand
sky-RA 4920 CG9339-RA 743..2515 17..1789 8835 99.9 Plus
sky-RH 5080 CG9339-RH 903..2675 17..1789 8835 99.9 Plus
sky-RB 5128 CG9339-RB 951..2723 17..1789 8835 99.9 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-28 05:44:35
Subject Length Description Subject Range Query Range Score Percent Strand
2L 23513712 2L 20877149..20877477 1029..701 1645 100 Minus
2L 23513712 2L 20873957..20874179 1638..1416 1115 100 Minus
2L 23513712 2L 20881987..20882194 575..368 1040 100 Minus
2L 23513712 2L 20882260..20882465 372..167 1030 100 Minus
2L 23513712 2L 20876912..20877085 1201..1028 870 100 Minus
2L 23513712 2L 20874271..20874432 1420..1259 810 100 Minus
2L 23513712 2L 20903015..20903166 168..17 760 100 Minus
2L 23513712 2L 20873740..20873891 1789..1638 730 98.7 Minus
2L 23513712 2L 20877553..20877678 700..575 630 100 Minus
2L 23513712 2L 20875080..20875138 1258..1200 295 100 Minus
Blast to na_te.dros performed on 2014-11-28 05:44:37 has no hits.

BS05025.complete Sim4 Records

Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-21 23:05:25 Download gff for BS05025.complete
Subject Subject Range Query Range Percent Splice Strand
CG9339-RA 1..1764 17..1780 100   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2010-07-28 16:35:43 Download gff for BS05025.complete
Subject Subject Range Query Range Percent Splice Strand
CG9339-RA 740..2501 17..1778 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 22:11:27 Download gff for BS05025.complete
Subject Subject Range Query Range Percent Splice Strand
sky-RB 951..2712 17..1778 100   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-09-04 16:47:36 Download gff for BS05025.complete
Subject Subject Range Query Range Percent Splice Strand
CG9339-RA 740..2501 17..1778 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-28 06:17:23 Download gff for BS05025.complete
Subject Subject Range Query Range Percent Splice Strand
sky-RB 951..2712 17..1778 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-28 06:17:23 Download gff for BS05025.complete
Subject Subject Range Query Range Percent Splice Strand
2L 20873957..20874177 1418..1638 100 <- Minus
2L 20874274..20874432 1259..1417 100 <- Minus
2L 20875080..20875136 1202..1258 100 <- Minus
2L 20876912..20877083 1030..1201 100 <- Minus
2L 20873751..20873890 1639..1778 100 <- Minus
2L 20877149..20877477 701..1029 100 <- Minus
2L 20877553..20877677 576..700 100 <- Minus
2L 20881987..20882191 371..575 100 <- Minus
2L 20882262..20882464 168..370 100 <- Minus
2L 20903016..20903166 17..167 100   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 22:11:27 Download gff for BS05025.complete
Subject Subject Range Query Range Percent Splice Strand
arm_2L 20903016..20903166 17..167 100   Minus
arm_2L 20882262..20882464 168..370 100 <- Minus
arm_2L 20881987..20882191 371..575 100 <- Minus
arm_2L 20873751..20873890 1639..1778 100 <- Minus
arm_2L 20873957..20874177 1418..1638 100 <- Minus
arm_2L 20874274..20874432 1259..1417 100 <- Minus
arm_2L 20875080..20875136 1202..1258 100 <- Minus
arm_2L 20876912..20877083 1030..1201 100 <- Minus
arm_2L 20877149..20877477 701..1029 100 <- Minus
arm_2L 20877553..20877677 576..700 100 <- Minus

BS05025.pep Sequence

Translation from 16 to 1779

> BS05025.pep
MPYHRGGDASSQADKLSGIVEESDLYEGFAPHVETSEIKTLDFYNLPKQT
GKEPALRSYTEIQQLLQQGKKRDVKNILRENSWPINSPIRAQLWPMLCGQ
HQTKQQMLDGFYWEMVHQVFGTTELSEKPIMLPAFVDATHCLPYHLTSTG
RAVADRIVNVLGYDCPDITYSPVLYPITSILLHFMSEEEAYICLAGLVGS
KEKVFINQTKLQHEVTWKTVMQIAKKHTKSATSYFQRICPGLKLERIFMD
WCWWILAGLPFQHLVRIMDCYFHEGIKVLYRVALVILNLFHKECQSNNEW
SPDNIKNDIGNALIKFCKKIPVSPAKLLHAAFSIRGLSTQYISRIFIKTE
MLLKSRSVLTSGSKQLIKSRSSDNLPTSQSQVNIQMMSHTLTIREHFGLP
GTKNFIKTWTDRQFLFTLWSWLPVRITMYQPVLLYTTEEHGCSLTTFYVR
VEQHEPTLLMIKTCNNEVFGAYCSSRWFERNVKDDKGQRQAYFGTGETFL
FSLYPERAKYPWVGIEGDKDLGHSSELFMAADSKMITIGGGEGQAIWMDE
NIRFGKTDSCKTFNNPPLCPSGDFEIRVLEVYGFVGI*

BS05025.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 18:38:57
Subject Length Description Subject Range Query Range Score Percent Strand
sky-PA 587 CG9339-PA 1..587 1..587 3146 100 Plus
sky-PH 587 CG9339-PH 1..587 1..587 3146 100 Plus
sky-PB 587 CG9339-PB 1..587 1..587 3146 100 Plus
sky-PK 597 CG9339-PK 1..597 1..587 3014 95.6 Plus
sky-PG 568 CG9339-PG 1..568 1..587 3005 96.8 Plus