Clone BS14027 Report

Clone Sequence Records

BS14027.3prime Sequence

468 bp (462 high quality bases) assembled on 2006-10-13

> BS14027.3prime
ATGGTCTAGAAAGCTTCTAACCGTACAGTCCACCTCCGGAGGCATTGGCG
GATGACGATGCAGAAGCGGATCCAGCGCCACCACGTCCGCCACCACTAGC
CGATGCCGAGGAAGAGGCTGAGGCCGCAGATGAGCCACCGACTGCAGGAC
GTCCACCACCGAATCCTGAGCCACCACCAAATCCAGGACCTCCACCAAAT
CCACCGCCGAATCCTGGGCCACCGCCGAATCCTGGGCGACCGCCGAAACC
TTGGCCACCGCCGAAACCTGGGCCACCGCCGAATCCTGGGCCACCGCCAA
AGCCTGGGCGACCGCCGAAACCTGGGCCACCGCCGAAACCTGGGCCACCG
CCAAACCCTGGGCCACCGCCGAATCCAGGACGACCTCCAAGGCCGCCCTG
GGCATTGGCATTAGCATTCGCGTTGGCGTTGGCGTTTCCGCCGAATTGAG
GCAGGGCCGATGCCACAG

BS14027.3prime Blast Records

Blast to dmel-all-CDS-r5.1.fasta performed 2007-05-11 11:44:08

Subject	Length	Description	Subject Range	Query Range	Score	Percent	Strand
CG7296-PA	486	CG7296-RA	35..486	468..17	2260	100	Minus

Blast to dmel-all-transcript-r6.02.fasta performed 2015-02-12 04:00:14

Subject	Length	Description	Subject Range	Query Range	Score	Percent	Strand
CG7296-RB	943	CG7296-RB	154..606	468..16	2265	100	Minus
CG7296-RA	599	CG7296-RA	77..529	468..16	2265	100	Minus
CG7296-RB	943	CG7296-RB	249..422	319..152	480	86.2	Minus
CG7296-RB	943	CG7296-RB	303..470	373..200	480	86.2	Minus
CG7296-RA	599	CG7296-RA	226..393	373..200	480	86.2	Minus
CG7296-RA	599	CG7296-RA	172..345	319..152	480	86.2	Minus
CG7296-RB	943	CG7296-RB	232..383	354..203	460	86.8	Minus
CG7296-RB	943	CG7296-RB	268..419	390..239	460	86.8	Minus
CG7296-RA	599	CG7296-RA	155..306	354..203	460	86.8	Minus
CG7296-RA	599	CG7296-RA	191..342	390..239	460	86.8	Minus
CG7296-RB	943	CG7296-RB	237..349	313..201	430	92	Minus
CG7296-RB	943	CG7296-RB	309..421	385..273	430	92	Minus
CG7296-RA	599	CG7296-RA	160..272	313..201	430	92	Minus
CG7296-RA	599	CG7296-RA	232..344	385..273	430	92	Minus
CG7296-RB	943	CG7296-RB	228..331	304..201	325	87.5	Minus
CG7296-RB	943	CG7296-RB	318..421	394..291	325	87.5	Minus
CG7296-RA	599	CG7296-RA	151..254	304..201	325	87.5	Minus
CG7296-RA	599	CG7296-RA	241..344	394..291	325	87.5	Minus
CG7296-RB	943	CG7296-RB	249..367	265..153	255	81.5	Minus
CG7296-RB	943	CG7296-RB	357..469	373..255	255	81.5	Minus
CG7296-RA	599	CG7296-RA	172..290	265..153	255	81.5	Minus
CG7296-RA	599	CG7296-RA	280..392	373..255	255	81.5	Minus

Blast to na_all.dmel.RELEASE6 performed 2015-02-12 04:00:12

Subject	Length	Description	Subject Range	Query Range	Score	Percent	Strand
2L	23513712	2L	10686461..10686913	16..468	2265	100	Plus
2L	23513712	2L	10686646..10686764	255..373	460	92.4	Plus
2L	23513712	2L	10686648..10686799	239..390	460	86.8	Plus
2L	23513712	2L	10686684..10686835	203..354	460	86.8	Plus
2L	23513712	2L	10686700..10686818	201..319	460	92.4	Plus
2L	23513712	2L	10686646..10686758	273..385	430	92	Plus
2L	23513712	2L	10686718..10686830	201..313	430	92	Plus
2L	23513712	2L	10686646..10686749	291..394	325	87.5	Plus
2L	23513712	2L	10686736..10686839	201..304	325	87.5	Plus
2L	23513712	2L	10686598..10686710	255..373	230	81.5	Plus
2L	23513712	2L	10686700..10686818	153..265	230	81.5	Plus

Blast to na_te.dros performed on 2015-02-12 04:00:13 has no hits.

BS14027.3prime Sim4 Records

Sim4 to dmel-all-CDS-r5.1.fasta performed 2007-05-11 13:46:57 Download gff for BS14027.3prime

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-PA	35..486	17..468	100		Minus

Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-28 21:33:29 Download gff for BS14027.3prime

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-RA	77..535	9..468	99		Minus

Sim4 to dmel-all-transcript-r6.02.fasta performed 2015-02-12 06:59:52 Download gff for BS14027.3prime

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-RA	77..535	9..468	99		Minus

Sim4 to na_all.dmel.RELEASE6 performed 2015-02-12 06:59:52 Download gff for BS14027.3prime

Subject	Subject Range	Query Range	Percent	Splice	Strand
2L	10686455..10686913	9..468	99		Plus

Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-28 21:33:29 Download gff for BS14027.3prime

Subject	Subject Range	Query Range	Percent	Splice	Strand
arm_2L	10686455..10686913	9..468	99		Plus

BS14027.5prime Sequence

516 bp (516 high quality bases) assembled on 2006-10-13

> BS14027.5prime
GAAGTTATCAGTCGACATGAAACTTTTCATCGCGCTCTCTGTTCTGGTGG
CTGTGGCATCGGCCCTGCCTCAATTCGGCGGAAACGCCAACGCCAACGCG
AATGCTAATGCCAATGCCCAGGGCGGCCTTGGAGGTCGTCCTGGATTCGG
CGGTGGCCCAGGGTTTGGCGGTGGCCCAGGTTTCGGCGGTGGCCCAGGTT
TCGGCGGTCGCCCAGGCTTTGGCGGTGGCCCAGGATTCGGCGGTGGCCCA
GGTTTCGGCGGTGGCCAAGGTTTCGGCGGTCGCCCAGGATTCGGCGGTGG
CCCAGGATTCGGCGGTGGATTTGGTGGAGGTCCTGGATTTGGTGGTGGCT
CAGGATTCGGTGGTGGACGTCCTGCAGTCGGTGGCTCATCTGCGGCCTCA
GCCTCTTCCTCGGCATCGGCTAGTGGTGGCGGACGTGGTGGCGCTGGATC
CGCTTCTGCATCGTCATCCGCCAATGCCTCCGGAGGTGGACTGTACGGTT
AGAAGCTTTCTAGACC

BS14027.5prime Blast Records

Blast to dmel-all-CDS-r5.1.fasta performed 2007-05-11 11:44:10

Subject	Length	Description	Subject Range	Query Range	Score	Percent	Strand
CG7296-PA	486	CG7296-RA	1..486	17..502	2430	100	Plus

Blast to dmel-all-transcript-r6.02.fasta performed 2015-02-03 06:47:29

Subject	Length	Description	Subject Range	Query Range	Score	Percent	Strand
CG7296-RB	943	CG7296-RB	118..606	15..503	2445	100	Plus
CG7296-RA	599	CG7296-RA	41..529	15..503	2445	100	Plus
CG7296-RB	943	CG7296-RB	249..422	200..367	480	86.2	Plus
CG7296-RB	943	CG7296-RB	303..470	146..319	480	86.2	Plus
CG7296-RA	599	CG7296-RA	172..345	200..367	480	86.2	Plus
CG7296-RA	599	CG7296-RA	226..393	146..319	480	86.2	Plus
CG7296-RB	943	CG7296-RB	232..383	165..316	460	86.8	Plus
CG7296-RB	943	CG7296-RB	268..419	129..280	460	86.8	Plus
CG7296-RA	599	CG7296-RA	155..306	165..316	460	86.8	Plus
CG7296-RA	599	CG7296-RA	191..342	129..280	460	86.8	Plus
CG7296-RB	943	CG7296-RB	237..349	206..318	430	92	Plus
CG7296-RB	943	CG7296-RB	309..421	134..246	430	92	Plus
CG7296-RA	599	CG7296-RA	160..272	206..318	430	92	Plus
CG7296-RA	599	CG7296-RA	232..344	134..246	430	92	Plus
CG7296-RB	943	CG7296-RB	228..331	215..318	325	87.5	Plus
CG7296-RB	943	CG7296-RB	318..421	125..228	325	87.5	Plus
CG7296-RA	599	CG7296-RA	151..254	215..318	325	87.5	Plus
CG7296-RA	599	CG7296-RA	241..344	125..228	325	87.5	Plus
CG7296-RB	943	CG7296-RB	249..367	254..366	255	81.5	Plus
CG7296-RB	943	CG7296-RB	357..469	146..264	255	81.5	Plus
CG7296-RA	599	CG7296-RA	172..290	254..366	255	81.5	Plus
CG7296-RA	599	CG7296-RA	280..392	146..264	255	81.5	Plus

Blast to na_all.dmel.RELEASE6 performed 2015-02-03 06:47:22

Subject	Length	Description	Subject Range	Query Range	Score	Percent	Strand
2L	23513712	2L	10686461..10686949	503..15	2445	100	Minus
2L	23513712	2L	10686646..10686764	264..146	460	92.4	Minus
2L	23513712	2L	10686648..10686799	280..129	460	86.8	Minus
2L	23513712	2L	10686684..10686835	316..165	460	86.8	Minus
2L	23513712	2L	10686700..10686818	318..200	460	92.4	Minus
2L	23513712	2L	10686646..10686758	246..134	430	92	Minus
2L	23513712	2L	10686718..10686830	318..206	430	92	Minus
2L	23513712	2L	10686646..10686749	228..125	325	87.5	Minus
2L	23513712	2L	10686736..10686839	318..215	325	87.5	Minus
2L	23513712	2L	10686598..10686710	264..146	230	81.5	Minus
2L	23513712	2L	10686700..10686818	366..254	230	81.5	Minus

Blast to na_te.dros performed on 2015-02-03 06:47:26 has no hits.

BS14027.5prime Sim4 Records

Sim4 to dmel-all-CDS-r5.1.fasta performed 2007-05-11 13:46:57 Download gff for BS14027.5prime

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-PA	1..486	17..502	100		Plus

Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-09 04:31:41 Download gff for BS14027.5prime

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-RA	38..535	12..510	98		Plus

Sim4 to dmel-all-transcript-r6.02.fasta performed 2015-02-03 07:39:31 Download gff for BS14027.5prime

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-RA	38..535	12..510	98		Plus

Sim4 to na_all.dmel.RELEASE6 performed 2015-02-03 07:39:31 Download gff for BS14027.5prime

Subject	Subject Range	Query Range	Percent	Splice	Strand
2L	10686455..10686952	12..510	98		Minus

Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-09 04:31:41 Download gff for BS14027.5prime

Subject	Subject Range	Query Range	Percent	Splice	Strand
arm_2L	10686455..10686952	12..510	98		Minus

BS14027.complete Sequence

518 bp assembled on 2006-11-01

GenBank Submission: FJ637586

> BS14027.complete
GAAGTTATCAGTCGACATGAAACTTTTCATCGCGCTCTCTGTTCTGGTGG
CTGTGGCATCGGCCCTGCCTCAATTCGGCGGAAACGCCAACGCCAACGCG
AATGCTAATGCCAATGCCCAGGGCGGCCTTGGAGGTCGTCCTGGATTCGG
CGGTGGCCCAGGGTTTGGCGGTGGCCCAGGTTTCGGCGGTGGCCCAGGTT
TCGGCGGTCGCCCAGGCTTTGGCGGTGGCCCAGGATTCGGCGGTGGCCCA
GGTTTCGGCGGTGGCCAAGGTTTCGGCGGTCGCCCAGGATTCGGCGGTGG
CCCAGGATTCGGCGGTGGATTTGGTGGAGGTCCTGGATTTGGTGGTGGCT
CAGGATTCGGTGGTGGACGTCCTGCAGTCGGTGGCTCATCTGCGGCCTCA
GCCTCTTCCTCGGCATCGGCTAGTGGTGGCGGACGTGGTGGCGCTGGATC
CGCTTCTGCATCGTCATCCGCCAATGCCTCCGGAGGTGGACTGTACGGTT
AGAAGCTTTCTAGACCAT

BS14027.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-27 19:51:21

Subject	Length	Description	Subject Range	Query Range	Score	Percent	Strand
CG7296-RB	486	CG7296-PB	1..486	17..502	2430	100	Plus
CG7296-RA	486	CG7296-PA	1..486	17..502	2430	100	Plus
CG7296-RB	486	CG7296-PB	113..264	165..316	460	86.8	Plus
CG7296-RB	486	CG7296-PB	130..248	200..318	460	92.4	Plus
CG7296-RB	486	CG7296-PB	149..300	129..280	460	86.8	Plus
CG7296-RB	486	CG7296-PB	184..302	146..264	460	92.4	Plus
CG7296-RA	486	CG7296-PA	113..264	165..316	460	86.8	Plus
CG7296-RA	486	CG7296-PA	130..248	200..318	460	92.4	Plus
CG7296-RA	486	CG7296-PA	149..300	129..280	460	86.8	Plus
CG7296-RA	486	CG7296-PA	184..302	146..264	460	92.4	Plus
CG7296-RB	486	CG7296-PB	190..302	134..246	430	92	Plus
CG7296-RB	486	CG7296-PB	118..230	206..318	430	92	Plus
CG7296-RA	486	CG7296-PA	118..230	206..318	430	92	Plus
CG7296-RA	486	CG7296-PA	190..302	134..246	430	92	Plus
CG7296-RB	486	CG7296-PB	109..212	215..318	325	87.5	Plus
CG7296-RB	486	CG7296-PB	199..302	125..228	325	87.5	Plus
CG7296-RA	486	CG7296-PA	109..212	215..318	325	87.5	Plus
CG7296-RA	486	CG7296-PA	199..302	125..228	325	87.5	Plus
CG7296-RB	486	CG7296-PB	130..248	254..366	230	81.5	Plus
CG7296-RB	486	CG7296-PB	238..350	146..264	230	81.5	Plus
CG7296-RA	486	CG7296-PA	238..350	146..264	230	81.5	Plus
CG7296-RA	486	CG7296-PA	130..248	254..366	230	81.5	Plus

Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-27 19:51:22

Subject	Length	Description	Subject Range	Query Range	Score	Percent	Strand
CG7296-RB	943	CG7296-RB	118..606	15..503	2445	100	Plus
CG7296-RA	599	CG7296-RA	41..529	15..503	2445	100	Plus
CG7296-RB	943	CG7296-RB	232..383	165..316	460	86.8	Plus
CG7296-RB	943	CG7296-RB	249..367	200..318	460	92.4	Plus
CG7296-RB	943	CG7296-RB	268..419	129..280	460	86.8	Plus
CG7296-RB	943	CG7296-RB	303..421	146..264	460	92.4	Plus
CG7296-RA	599	CG7296-RA	155..306	165..316	460	86.8	Plus
CG7296-RA	599	CG7296-RA	172..290	200..318	460	92.4	Plus
CG7296-RA	599	CG7296-RA	191..342	129..280	460	86.8	Plus
CG7296-RA	599	CG7296-RA	226..344	146..264	460	92.4	Plus
CG7296-RB	943	CG7296-RB	237..349	206..318	430	92	Plus
CG7296-RB	943	CG7296-RB	309..421	134..246	430	92	Plus
CG7296-RA	599	CG7296-RA	160..272	206..318	430	92	Plus
CG7296-RA	599	CG7296-RA	232..344	134..246	430	92	Plus
CG7296-RB	943	CG7296-RB	228..331	215..318	325	87.5	Plus
CG7296-RB	943	CG7296-RB	318..421	125..228	325	87.5	Plus
CG7296-RA	599	CG7296-RA	151..254	215..318	325	87.5	Plus
CG7296-RA	599	CG7296-RA	241..344	125..228	325	87.5	Plus
CG7296-RB	943	CG7296-RB	357..469	146..264	230	81.5	Plus
CG7296-RB	943	CG7296-RB	249..367	254..366	230	81.5	Plus
CG7296-RA	599	CG7296-RA	172..290	254..366	230	81.5	Plus
CG7296-RA	599	CG7296-RA	280..392	146..264	230	81.5	Plus

Blast to na_all.dmel.RELEASE6 performed 2014-11-27 19:51:18

Subject	Length	Description	Subject Range	Query Range	Score	Percent	Strand
2L	23513712	2L	10686461..10686949	503..15	2445	100	Minus
2L	23513712	2L	10686684..10686835	316..165	460	86.8	Minus
2L	23513712	2L	10686700..10686818	318..200	460	92.4	Minus
2L	23513712	2L	10686646..10686764	264..146	460	92.4	Minus
2L	23513712	2L	10686648..10686799	280..129	460	86.8	Minus
2L	23513712	2L	10686646..10686758	246..134	430	92	Minus
2L	23513712	2L	10686718..10686830	318..206	430	92	Minus
2L	23513712	2L	10686646..10686749	228..125	325	87.5	Minus
2L	23513712	2L	10686736..10686839	318..215	325	87.5	Minus
2L	23513712	2L	10686598..10686710	264..146	230	81.5	Minus
2L	23513712	2L	10686700..10686818	366..254	230	81.5	Minus

Blast to na_te.dros performed on 2014-11-27 19:51:20 has no hits.

BS14027.complete Sim4 Records

Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-21 23:38:13 Download gff for BS14027.complete

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-RA	1..486	17..502	100		Plus

Sim4 to dmel-all-transcript-r5.12.fasta performed 2008-11-11 03:11:30 Download gff for BS14027.complete

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-RA	37..534	12..510	98		Plus

Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 18:59:50 Download gff for BS14027.complete

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-RA	43..528	17..502	100		Plus

Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-21 23:38:13 Download gff for BS14027.complete

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-RA	37..534	12..510	98		Plus

Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-27 21:09:36 Download gff for BS14027.complete

Subject	Subject Range	Query Range	Percent	Splice	Strand
CG7296-RA	43..528	17..502	100		Plus

Sim4 to na_all.dmel.RELEASE6 performed 2014-11-27 21:09:36 Download gff for BS14027.complete

Subject	Subject Range	Query Range	Percent	Splice	Strand
2L	10686462..10686947	17..502	100		Minus

Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 18:59:50 Download gff for BS14027.complete

Subject	Subject Range	Query Range	Percent	Splice	Strand
arm_2L	10686462..10686947	17..502	100		Minus

BS14027.pep Sequence

Translation from 16 to 501

> BS14027.pep
MKLFIALSVLVAVASALPQFGGNANANANANANAQGGLGGRPGFGGGPGF
GGGPGFGGGPGFGGRPGFGGGPGFGGGPGFGGGQGFGGRPGFGGGPGFGG
GFGGGPGFGGGSGFGGGRPAVGGSSAASASSSASASGGGRGGAGSASASS
SANASGGGLYG*

BS14027.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 17:15:59

Subject	Length	Description	Subject Range	Query Range	Score	Percent	Strand
CG7296-PB	161	CG7296-PB	1..161	1..161	871	100	Plus
CG7296-PA	161	CG7296-PA	1..161	1..161	871	100	Plus
CG7299-PC	177	CG7299-PC	1..161	1..158	401	55.7	Plus
CG7299-PA	177	CG7299-PA	1..161	1..158	401	55.7	Plus
CG7294-PA	127	CG7294-PA	1..127	1..161	368	58.9	Plus

Library:	BS
Tissue Source:	D. melanogaster
Created by:	Joe Carlson
Date Registered:	2004-06-03
Comments:	Infusion clones with native stop reading frames
Original Plate Number:	140
Well:	27
Vector:	pDNR-Dual
Associated Gene/Transcript	CG7296-RA
Protein status:	BS14027.pep: full length peptide match
Sequenced Size:	16