Clone BS20725 Report

Search the DGRC for BS20725

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:207
Well:25
Vector:pDNR-Dual
Associated Gene/TranscriptPfk-RC
Protein status:BS20725.pep: full length peptide match
Sequenced Size:2399

Clone Sequence Records

BS20725.complete Sequence

2399 bp assembled on 2011-02-17

GenBank Submission: KX802000

> BS20725.complete
GAAGTTATCAGTCGACATGAATTCCGAGATTAATCAACGATTCCTGGCCC
GCGGCTCGCAGAAGGACAAAGGACTGGCGGTCTTCACCAGCGGCGGGGAT
TCCCAGGGCATGAATGCCGCCGTGCGAGCCTGTGTCCGTATGGCCATCTA
TTTGGGCTGCAAGGTCTACTTCATTCGTGAAGGCTACCAGGGCATGGTGG
ATGGTGGCGACTGCATCCAGGAAGCCAACTGGGCCTCAGTCTCATCCATT
ATCCATCGTGGTGGCACCATCATTGGCTCCGCCCGTTGCCAGGACTTCCG
TGAGCGTCAGGGTCGCTTGAAAGCCGCTAACAACCTGATTCAGCGAGGAA
TCACCAATCTGGTGGTCATTGGAGGCGATGGCTCCCTCACGGGCGCCAAT
CTGTTCCGTCAGGAGTGGTCCAGCCTGTTGGACGAGCTGGTCAAGAACAA
GACCATTACCACCGAGCAGCAGGAGAAGTTCAATGTCCTGCACATCGTTG
GATTGGTTGGCTCCATCGACAACGATTTCTGTGGCACTGACATGACCATC
GGCACGGACACGGCACTACATCGCATCATCGAGGCAATCGATGCCATCTC
CAGTACAGCCTACTCCCATCAGCGCACCTTCATCATGGAGGTCATGGGTC
GTCATTGCGGTTACTTAGCCATATCGGCAGCCATAGCCACAGAGGCTGAT
TTTATGTTCATACCCGAGGAGCCGGTGTCAGTCAATTGGAAGGACGAGAT
CTGTGTCAAGCTCCACCAGGAGCGATCTGCTGGCCAGCGTCTGAACATCG
TGATTGTGGCCGAGGGAGCCATGGATCGCGAGGGTCATCCCATCACAGCC
GAGGATGTAAAGAAGGTGATCGACGAGCGTCTGAAGCACGATGCCCGCAT
CACTGTCTTGGGTCACGTGCAGCGCGGTGGCAATCCCAGTGCCTTCGATC
GTATTTTGGCTTGCCGCATGGGAGCTGAGGCCACTTTGGCCCTGATGGAG
GCCACCAAGGACTCGGTGCCAGTGGTCATCTCTCTGGACGGCAACCAGGC
GGTTCGCGTGCCGCTGATGGAGTGCGTAGAGCGCACCCAGGCTGTGGCCA
AGGCCATGGCGGAGAAACGCTGGGCGGATGCCGTCAAGCTGCGCGGACGC
TCCTTCGAGCGGAATCTGGAGACCTACAAGATGCTGACGCGCTTGAAGCC
GCCCAAGGAGAACTTCGACGCCGATGGCAAGGGAATCGAAGGATACCGCC
TAGCTGTGATGCATATTGGTGCTCCGGCTTGCGGTATGAATGCCGCTGTG
CGCAGCTTCGTGCGCAATGCCATCTACCGCGGTGATGTGGTTTATGGAAT
CAACGACGGTGTCGAGGGTCTGATTGCCGGAAATGTCCGCGAGCTGGGCT
GGTCGGATGTCTCAGGATGGGTTGGTCAGGGTGGCGCCTACTTGGGTACC
AAACGCACACTGCCTGAGGGCAAGTTCAAGGAGATCGCCGCTCGTCTCAA
GGAGTTTAAGATCCAGGGTCTCCTGATCATTGGTGGCTTTGAGAGTTACC
ATGCCGCCGGACAGATCGCCGATCAGCGGGACAACTACCCACAGTTCTGC
ATCCCCATTGTGGTTATTCCATCGACGATTTCGAACAATGTGCCCGGCAC
AGAATTTTCTCTGGGATGCGACACCGGTTTAAATGAGATTACGGAGATTT
GCGACCGTATCCGTCAGTCGGCACAGGGAACCAAGCGCCGAGTGTTCGTC
ATTGAGACGATGGGTGGCTACTGTGGCTATTTGGCCACCTTGGCCGGCTT
GGCCGGCGGAGCTGATGCCGCCTACATCTACGAGGAGAAGTTCTCCATCA
AAGACCTGCAGCAGGATGTCTACCACATGGCCTCCAAGATGGCCGAGGGC
GTCTCCCGCGGTCTAATCCTGCGAAACGAGAAGGCTAGCGAGAACTACAG
CACGGACTTCATTTACCGCCTGTACTCGGAGGAGGGCAAGGGCCTCTTCA
CCTGCCGTATGAACATCCTGGGTCACATGCAGCAGGGCGGCTCACCCACT
CCCTTCGACCGCAACATGGGCACCAAAATGGCCGCCAAGTGTGTCGACTG
GCTGGCCGCCCAGATCAAGGCGAACATCGACGCCAACGGCGTAGTCAACT
GCAAGTCCCCGGACACCGCCACGCTGCTGGGAATCGTGTCGCGGCAGTAC
CGCTTCTCACCGCTGGTCGACCTCATTGCAGAGACGAACTTCGATCAACG
CATCCCGAAGAAGCAGTGGTGGCTGCGCCTGCGTCCCCTGCTGCGCATCC
TGGCCAAGCACGATTCCGCCTACGAGGAGGAGGGTATGTACATCACCGTC
GAGGAGGAGTGTGACACTGACGCCGTCGCCTAAAAGCTTTCTAGACCAT

BS20725.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-27 07:02:28
Subject Length Description Subject Range Query Range Score Percent Strand
Pfk-RC 2367 CG4001-PC 1..2367 17..2383 11835 100 Plus
Pfk-RB 2367 CG4001-PB 1..2367 17..2383 11160 98.1 Plus
Pfk-RA 2853 CG4001-PA 532..2853 62..2383 10935 98.1 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-27 07:02:29
Subject Length Description Subject Range Query Range Score Percent Strand
Pfk-RC 3188 CG4001-RC 560..2926 17..2383 11835 100 Plus
Pfk-RB 3508 CG4001-RB 560..2926 17..2383 11160 98.1 Plus
Pfk-RA 3217 CG4001-RA 634..2955 62..2383 10935 98.1 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-27 07:02:26
Subject Length Description Subject Range Query Range Score Percent Strand
2R 25286936 2R 10115814..10116657 1401..2244 4220 100 Plus
2R 25286936 2R 10115314..10115759 958..1403 2230 100 Plus
2R 25286936 2R 10112612..10112960 159..507 1745 100 Plus
2R 25286936 2R 10114910..10115106 763..959 970 99.5 Plus
2R 25286936 2R 10113015..10113170 505..660 780 100 Plus
2R 25286936 2R 10111429..10111577 17..165 745 100 Plus
2R 25286936 2R 10116737..10116875 2245..2383 695 100 Plus
2R 25286936 2R 10114125..10114235 660..770 555 100 Plus
Blast to na_te.dros performed on 2014-11-27 07:02:27 has no hits.

BS20725.complete Sim4 Records

Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-02-17 10:41:37 Download gff for BS20725.complete
Subject Subject Range Query Range Percent Splice Strand
Pfk-RC 533..2897 17..2381 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 05:03:11 Download gff for BS20725.complete
Subject Subject Range Query Range Percent Splice Strand
Pfk-RC 560..2924 17..2381 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-27 08:01:28 Download gff for BS20725.complete
Subject Subject Range Query Range Percent Splice Strand
Pfk-RC 560..2924 17..2381 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-27 08:01:28 Download gff for BS20725.complete
Subject Subject Range Query Range Percent Splice Strand
2R 10115815..10116657 1402..2244 100 -> Plus
2R 10111429..10111575 17..163 100 -> Plus
2R 10112617..10112958 164..505 100 -> Plus
2R 10113016..10113170 506..660 100 -> Plus
2R 10114126..10114234 661..769 100 -> Plus
2R 10114917..10115105 770..958 100 -> Plus
2R 10115315..10115757 959..1401 100 -> Plus
2R 10116737..10116873 2245..2381 100   Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 05:03:11 Download gff for BS20725.complete
Subject Subject Range Query Range Percent Splice Strand
arm_2R 6002422..6002610 770..958 100 -> Plus
arm_2R 6000122..6000463 164..505 100 -> Plus
arm_2R 5998934..5999080 17..163 100 -> Plus
arm_2R 6000521..6000675 506..660 100 -> Plus
arm_2R 6001631..6001739 661..769 100 -> Plus
arm_2R 6002820..6003262 959..1401 100 -> Plus
arm_2R 6003320..6004162 1402..2244 100 -> Plus
arm_2R 6004242..6004378 2245..2381 100   Plus

BS20725.pep Sequence

Translation from 16 to 2382

> BS20725.pep
MNSEINQRFLARGSQKDKGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKV
YFIREGYQGMVDGGDCIQEANWASVSSIIHRGGTIIGSARCQDFRERQGR
LKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTE
QQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYS
HQRTFIMEVMGRHCGYLAISAAIATEADFMFIPEEPVSVNWKDEICVKLH
QERSAGQRLNIVIVAEGAMDREGHPITAEDVKKVIDERLKHDARITVLGH
VQRGGNPSAFDRILACRMGAEATLALMEATKDSVPVVISLDGNQAVRVPL
MECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENF
DADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVE
GLIAGNVRELGWSDVSGWVGQGGAYLGTKRTLPEGKFKEIAARLKEFKIQ
GLLIIGGFESYHAAGQIADQRDNYPQFCIPIVVIPSTISNNVPGTEFSLG
CDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGAD
AAYIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIY
RLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQI
KANIDANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETNFDQRIPKKQ
WWLRLRPLLRILAKHDSAYEEEGMYITVEEECDTDAVA*

BS20725.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-27 19:18:26
Subject Length Description Subject Range Query Range Score Percent Strand
Pfk-PC 788 CG4001-PC 1..788 1..788 4086 100 Plus
Pfk-PB 788 CG4001-PB 1..788 1..788 4003 97.8 Plus
Pfk-PA 950 CG4001-PA 178..950 16..788 3929 97.8 Plus
Pfk-PD 950 CG4001-PD 178..950 16..788 3922 97.4 Plus
Pfk-PE 922 CG4001-PE 184..922 50..788 3746 97.3 Plus