Clone BS20821 Report

Search the DGRC for BS20821

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:208
Well:21
Vector:pDNR-Dual
Associated Gene/TranscriptCG3625-RA
Protein status:BS20821.pep: gold
Sequenced Size:770

Clone Sequence Records

BS20821.complete Sequence

770 bp assembled on 2011-01-18

GenBank Submission: KX805146

> BS20821.complete
GAAGTTATCAGTCGACATGGCCAAGCCCAAGTCAAAATCGACTAGCAAAG
GTTCCACTTCGCCGGGTTTTAACAAGGCGGTGCAGTTGCTCCTCCATTTT
ACAGCCGTGCTGCAGTTCAACTATGGCGTGTACATCTTCCACACACTCGA
CATGCCCAACACGGTGCCCTTCGGCGGAAAATTTAAGTTCCTAACTTTTA
TTTGTGCGATAATTCAGGCACTGTACTATATTGTATCGCTGGTCAACGAT
TTCGTGGGCACTAATGAGCTGACACCCAAGAAACCTCCGGCGGTTCGCAG
GTTCAAGGACTGGCTGATGGCTACATTGGCCTTTCCAGTGGCTATCAATG
TGGGCGTGACCTTCTGGACACTGTACGCTATCGACAGGGAGCTAGTCTTC
CCCAAGGTTCTGGACCCCGTGTTCCCCAGCTGGCTCAACCACGTCCTGCA
CACCAACATAGTGGTTTTCATCATCCTGGAGCTGTTCATCTCGTACAGGT
CGTACCCAAAACGAAGCCAAGGACTCGCAGGACTGGCCATTTTCATGGGC
GCCTACCTGGTTTGGATTCACGTGGTGAAGCACTACTCGGGTGTGTGGGT
GTATCCAGTGCTCGAGGTTCTCCAGCTTCCGCAACGCATCCTGTTTTTTG
CGGCCGTCGTTGGATTCACGCTGTCACTTTACCTGCTGGGCGAGTTCCTC
AACAATACCGTCTGGGCCAAGGAAGTGAAGTTGGCCAAGCGCAAATCCAA
CTAGAAGCTTTCTAGACCAT

BS20821.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-26 16:13:56
Subject Length Description Subject Range Query Range Score Percent Strand
CG3625-RA 738 CG3625-PA 1..738 17..754 3690 100 Plus
CG3625-RB 747 CG3625-PB 198..747 205..754 2750 100 Plus
CG3625-RE 744 CG3625-PE 198..744 208..754 2735 100 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-26 16:13:58
Subject Length Description Subject Range Query Range Score Percent Strand
CG3625-RA 995 CG3625-RA 32..771 15..754 3700 100 Plus
CG3625-RB 1341 CG3625-RB 291..840 205..754 2750 100 Plus
CG3625-RE 1401 CG3625-RE 354..900 208..754 2735 100 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-26 16:13:54
Subject Length Description Subject Range Query Range Score Percent Strand
2L 23513712 2L 283886..284213 754..427 1640 100 Minus
2L 23513712 2L 284748..284969 429..208 1110 100 Minus
2L 23513712 2L 286303..286500 212..15 975 99.5 Minus
Blast to na_te.dros performed on 2014-11-26 16:13:55 has no hits.

BS20821.complete Sim4 Records

Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-01-20 15:39:22 Download gff for BS20821.complete
Subject Subject Range Query Range Percent Splice Strand
CG3625-RA 30..767 17..754 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-03 23:51:06 Download gff for BS20821.complete
Subject Subject Range Query Range Percent Splice Strand
CG3625-RA 34..771 17..754 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-26 16:59:15 Download gff for BS20821.complete
Subject Subject Range Query Range Percent Splice Strand
CG3625-RA 34..771 17..754 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-26 16:59:15 Download gff for BS20821.complete
Subject Subject Range Query Range Percent Splice Strand
2L 283886..284210 430..754 100 <- Minus
2L 284748..284968 209..429 100 <- Minus
2L 286307..286498 17..208 100   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-03 23:51:06 Download gff for BS20821.complete
Subject Subject Range Query Range Percent Splice Strand
arm_2L 283886..284210 430..754 100 <- Minus
arm_2L 284748..284968 209..429 100 <- Minus
arm_2L 286307..286498 17..208 100   Minus

BS20821.pep Sequence

Translation from 16 to 753

> BS20821.pep
MAKPKSKSTSKGSTSPGFNKAVQLLLHFTAVLQFNYGVYIFHTLDMPNTV
PFGGKFKFLTFICAIIQALYYIVSLVNDFVGTNELTPKKPPAVRRFKDWL
MATLAFPVAINVGVTFWTLYAIDRELVFPKVLDPVFPSWLNHVLHTNIVV
FIILELFISYRSYPKRSQGLAGLAIFMGAYLVWIHVVKHYSGVWVYPVLE
VLQLPQRILFFAAVVGFTLSLYLLGEFLNNTVWAKEVKLAKRKSN*

BS20821.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-27 13:03:00
Subject Length Description Subject Range Query Range Score Percent Strand
CG3625-PA 245 CG3625-PA 1..245 1..245 1280 100 Plus
CG3625-PB 248 CG3625-PB 3..248 8..245 1049 84.1 Plus
CG3625-PE 247 CG3625-PE 15..247 25..245 967 82.1 Plus
CG3625-PC 247 CG3625-PC 15..247 25..245 967 82.1 Plus
CG11601-PC 275 CG11601-PC 26..270 8..243 572 42.9 Plus