Clone BS25728 Report

Search the DGRC for BS25728

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:257
Well:28
Vector:pDNR-Dual
Associated Gene/Transcriptpnr-RB
Protein status:BS25728.pep: full length peptide match
Sequenced Size:1499

Clone Sequence Records

BS25728.complete Sequence

1499 bp assembled on 2011-08-02

GenBank Submission: KX806541

> BS25728.complete
GAAGTTATCAGTCGACATGTACCACAGTAGCGCCGTTGCGGCATACACGG
ACCTGGCTGCCGCCGGTAGTGCCGCAAGCGCCGGAGTCGGAGTCGGGGTG
TCCGGCTATCACCAGCAGGCGGTAAACGCTCCCGTTTACGTGCCATCCAA
CCGACAGTACAACCACGTGGCCGCTCATTTTGGAAGCGCTGCGGCGCAGA
ATGCCTGGACCACGGAAGGATTCGGATCGGCGCATGCACAGTTCTACTCG
CCAAACGCCGCTGTGATGATGGGCTCGTGGCGCAGTGCCTACGATCCGTC
GGGCTTCCAAAGATCCTCGCCCTACGAGAGCGCTATGGACTTCCAGTTTG
GCGAGGGACGCGAGTGCGTCAATTGCGGTGCCATATCGACTCCGTTGTGG
CGACGGGATGGAACCGGACACTATCTGTGCAACGCCTGCGGACTGTACCA
TAAGATGAACGGAATGAACCGGCCGCTGATCAAGCCTAGCAAGCGCCTGG
TGAGTGCAACTGCCACTCGGAGGATGGGCTTGTGCTGCACCAACTGCGGC
ACTCGGACCACCACACTTTGGAGACGCAACAACGATGGCGAACCCGTGTG
CAATGCCTGCGGACTGTACTACAAGTTGCACGGTGTTAACAGACCACTGG
CCATGAGGAAGGATGGCATTCAGACCAGGAAACGAAAGCCCAAGAAAACG
GGAAGTGGTTCGGCGGTGGGAGCGGGCACTGGATCCGGAACAGGCTCCAC
TTTGGAGGCCATCAAGGAGTGCAAGGAGGAGCATGATCTCAAACCCTCGC
TCAGCCTGGAGCGCCATAGCCTCAGTAAGCTGCACACGGATATGAAGAGC
GGCACCAGCAGTAGTAGCACCTTGATGGGACATCACAGCGCGCAGCAGCA
GCAGCAGCAGCAACAGCAACAACAACAGCAACAGCAGCAGCAGCAGCAGC
AATCGGCACACCAACAATGCTTTCCCCTTTACGGACAGACGACGACGCAA
CAGCAGCACCAGCAACATGGCCACAGCATGACATCATCCTCTGGACAGGC
ACATCTGAGTGCCCGCCACCTTCACGGAGCGGCAGGAACGCAGCTCTACA
CGCCCGGCAGCAGTTCCGGCGGAGGCAGTGCGTCTGCCTACACATCGCAC
AGTGCGGAGACGCCGGCCCTGAGCAACGGAACTCCCTCGCCCCATTACCA
GCACCACCACCACCTCGGCGGAACCCATGGACACCATGTGACGGCAGCGG
CGGCACATCATCATTTCCATGCCGCTGCCGCTGTGGCCGCCTACGGCGTA
AAGACAGAGGCGAGTGCCACCAACTACGACTATGTGAACAACTGCTACTT
TGGGGGCACCTTCGGTGCGCTGGGAGGAGCTGCAACCACAACCGCCATGG
CTGGCGGAGCGGCCAGTGAACTGGCTGGCTACCATCACCAGCACAACGTC
ATCCAGGCAGCCAAACTGATGGCCACCTCGTAAAAGCTTTCTAGACCAT

BS25728.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-26 22:33:26
Subject Length Description Subject Range Query Range Score Percent Strand
pnr-RD 1467 CG3978-PD 1..1467 17..1483 7320 99.9 Plus
pnr-RA 1623 CG3978-PA 157..1623 17..1483 7320 99.9 Plus
pnr-RB 1467 CG3978-PB 1..1467 17..1483 7320 99.9 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-26 22:33:28
Subject Length Description Subject Range Query Range Score Percent Strand
pnr-RD 3840 CG3978-RD 890..2357 17..1484 7325 99.9 Plus
pnr-RA 2720 CG3978-RA 651..2118 17..1484 7325 99.9 Plus
pnr-RB 2786 CG3978-RB 717..2184 17..1484 7325 99.9 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-26 22:33:24
Subject Length Description Subject Range Query Range Score Percent Strand
3R 32079331 3R 16039614..16040313 785..1484 3500 100 Plus
3R 32079331 3R 16038716..16039207 17..508 2460 100 Plus
3R 32079331 3R 16039270..16039546 509..785 1370 99.6 Plus
3R 32079331 3R 8160976..8161109 486..353 370 85.1 Minus
3R 32079331 3R 16018047..16018168 575..696 265 81.1 Plus
Blast to na_te.dros performed 2014-11-26 22:33:25
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6716..6903 840..1034 334 68.4 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2312..2534 892..1113 300 64 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6724..6868 892..1032 273 69.2 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6720..6825 918..1028 173 65.8 Plus
roo 9092 roo DM_ROO 9092bp 1049..1165 900..1015 159 60.7 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2306..2469 928..1089 148 58.7 Plus
BS 5142 BS BS 5142bp 2012..2125 911..1035 140 62.4 Plus

BS25728.complete Sim4 Records

Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-08-02 12:42:44 Download gff for BS25728.complete
Subject Subject Range Query Range Percent Splice Strand
pnr-RA 847..2311 17..1481 99   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 04:01:56 Download gff for BS25728.complete
Subject Subject Range Query Range Percent Splice Strand
pnr-RB 717..2181 17..1481 99   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-26 23:04:17 Download gff for BS25728.complete
Subject Subject Range Query Range Percent Splice Strand
pnr-RB 717..2181 17..1481 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-26 23:04:17 Download gff for BS25728.complete
Subject Subject Range Query Range Percent Splice Strand
3R 16038716..16039207 17..508 100 -> Plus
3R 16039270..16039546 509..785 99 -> Plus
3R 16039615..16040310 786..1481 100   Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 04:01:56 Download gff for BS25728.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3R 11864438..11864929 17..508 100 -> Plus
arm_3R 11864992..11865268 509..785 99 -> Plus
arm_3R 11865337..11866032 786..1481 100   Plus

BS25728.pep Sequence

Translation from 16 to 1482

> BS25728.pep
MYHSSAVAAYTDLAAAGSAASAGVGVGVSGYHQQAVNAPVYVPSNRQYNH
VAAHFGSAAAQNAWTTEGFGSAHAQFYSPNAAVMMGSWRSAYDPSGFQRS
SPYESAMDFQFGEGRECVNCGAISTPLWRRDGTGHYLCNACGLYHKMNGM
NRPLIKPSKRLVSATATRRMGLCCTNCGTRTTTLWRRNNDGEPVCNACGL
YYKLHGVNRPLAMRKDGIQTRKRKPKKTGSGSAVGAGTGSGTGSTLEAIK
ECKEEHDLKPSLSLERHSLSKLHTDMKSGTSSSSTLMGHHSAQQQQQQQQ
QQQQQQQQQQQQSAHQQCFPLYGQTTTQQQHQQHGHSMTSSSGQAHLSAR
HLHGAAGTQLYTPGSSSGGGSASAYTSHSAETPALSNGTPSPHYQHHHHL
GGTHGHHVTAAAAHHHFHAAAAVAAYGVKTEASATNYDYVNNCYFGGTFG
ALGGAATTTAMAGGAASELAGYHHQHNVIQAAKLMATS*

BS25728.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-29 07:10:07
Subject Length Description Subject Range Query Range Score Percent Strand
pnr-PD 488 CG3978-PD 1..488 1..488 2622 100 Plus
pnr-PA 540 CG3978-PA 53..540 1..488 2622 100 Plus
pnr-PB 488 CG3978-PB 1..488 1..488 2622 100 Plus
pnr-PC 537 CG3978-PC 53..537 1..488 2596 99.4 Plus
srp-PE 731 CG3992-PE 189..571 93..432 582 40.7 Plus