Clone BS28316 Report

Search the DGRC for BS28316

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:283
Well:16
Vector:pDNR-Dual
Associated Gene/TranscriptCG10195-RB
Protein status:BS28316.pep: full length peptide match
Sequenced Size:1118

Clone Sequence Records

BS28316.complete Sequence

1118 bp assembled on 2012-03-08

GenBank Submission: KX800935

> BS28316.complete
GAAGTTATCAGTCGACATGAGCCAACCTGCATCTCCTACCAAGTGGCGCA
CCTCGGCCAGTGTAATCCTGGTGTCCAGAGATGATGGCAATAACAAAGAT
TACAAGGTGCTTATGCTAAAACGCAGTGATGCCACGGCGATTGCGCTCAA
TCAGACTGTATTTCCCGGTGGCCTCTTGGACTCTGGGGCCGATGAAAGCG
TCGCCTGGCTGCACTACCTCGAGGAGTTTGGTGTTCCACAAGAGGCACTA
CGCCGCCTGGTTCTCATCCGCGAAGATCGTCCTGCCATTCTGGCGCCCCA
GGGCACTGGCTGCTACGATCGGTTCTTTAAGCGTTCCCGCATTTGGGCTC
GGGAGATTACGCTGCGACTAACCGCCGTGAGGGAATGCTTTGAAGAGGTG
GGTCTGTTGCTTTGCCGGAGTCGGAGTCAACTGGATTTTGGTGCGGTAAC
TTGCGCTCAGGCTGTTCCAGACCTGGAGTCGTGGCAGCGAAGGGTGCACA
ACAAGCCGGCTGAGTTTCTAACTCTCTGCCGCGAGCTAAATGTAGTTCCA
GATTTGTGGGCTCTGCATGAGTGGTCTGCCTGGGCGAGTCCAGGATTCAT
ACGCAAAGGGCATGAAACCGTTTTCTTCATGGCTTTTGTGGACAAGCAGC
CCGAATTGCTGGAAGAACCTTCCGAGGTCAAGGAAACGCTGTGGCTGACA
CCCGTTGAACTGCTGCGACTGGCTGATTTGGGCAATGTTTGGTTCATGCC
ACCACAGGTCTACGAATTGTCCCGGTTGATGGGAATTAAGGCGTATCAAT
CCCTGTTGGAATTTGCCATCAAGCGCAGCGGATTGGGAACGACCATGTTC
TTGCCGATTGGTTATAACTGCCAGGGCTCTATGGTTTTCGTCTTACCTGG
CGATGACTTCTATGTTCCCGAACCTCACTTGGTTCACGAAATAATCAGTT
TTCCGGGATCAGAAGAAGAGTTTAGGGCTCGTTCAAAGCATCTGCATCGC
TATACCTATGGTCCTGGTGTTCGAAACTTGGAGTTAAATATTGCGCCGCC
AAATGGACACTTGAAACCGCTGAAATTTCAGGCGGAGCGCCAAAAGCTGT
AAAAGTTTTCTAGACCAT

BS28316.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-28 05:47:23
Subject Length Description Subject Range Query Range Score Percent Strand
CG10195-RB 1086 CG10195-PB 1..1086 17..1102 5430 100 Plus
CG10195-RC 1086 CG10195-PC 1..1086 17..1102 5430 100 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-28 05:47:24
Subject Length Description Subject Range Query Range Score Percent Strand
CG10195-RB 1473 CG10195-RB 105..1190 17..1102 5430 100 Plus
CG10195-RC 2118 CG10195-RC 105..1190 17..1102 5430 100 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-28 05:47:21
Subject Length Description Subject Range Query Range Score Percent Strand
2L 23513712 2L 19483888..19484147 610..351 1300 100 Minus
2L 23513712 2L 19484213..19484460 352..105 1240 100 Minus
2L 23513712 2L 19483426..19483635 900..691 1050 100 Minus
2L 23513712 2L 19483160..19483363 1102..899 1020 100 Minus
2L 23513712 2L 19484511..19484601 107..17 455 100 Minus
2L 23513712 2L 19483692..19483774 691..609 415 100 Minus
Blast to na_te.dros performed on 2014-11-28 05:47:22 has no hits.

BS28316.complete Sim4 Records

Sim4 to dmel-all-transcript-r5.32.fasta performed 2012-03-08 17:47:24 Download gff for BS28316.complete
Subject Subject Range Query Range Percent Splice Strand
CG10195-RB 102..1185 17..1100 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 22:32:25 Download gff for BS28316.complete
Subject Subject Range Query Range Percent Splice Strand
CG10195-RC 105..1188 17..1100 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-28 06:18:21 Download gff for BS28316.complete
Subject Subject Range Query Range Percent Splice Strand
CG10195-RC 105..1188 17..1100 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-28 06:18:21 Download gff for BS28316.complete
Subject Subject Range Query Range Percent Splice Strand
2L 19483162..19483362 900..1100 100 <- Minus
2L 19483427..19483634 692..899 100 <- Minus
2L 19483692..19483773 610..691 100 <- Minus
2L 19483889..19484146 352..609 100 <- Minus
2L 19484214..19484458 107..351 100 <- Minus
2L 19484508..19484601 17..106 95   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 22:32:25 Download gff for BS28316.complete
Subject Subject Range Query Range Percent Splice Strand
arm_2L 19483162..19483362 900..1100 100 <- Minus
arm_2L 19483427..19483634 692..899 100 <- Minus
arm_2L 19483692..19483773 610..691 100 <- Minus
arm_2L 19483889..19484146 352..609 100 <- Minus
arm_2L 19484214..19484458 107..351 100 <- Minus
arm_2L 19484508..19484601 17..106 95   Minus

BS28316.pep Sequence

Translation from 16 to 1101

> BS28316.pep
MSQPASPTKWRTSASVILVSRDDGNNKDYKVLMLKRSDATAIALNQTVFP
GGLLDSGADESVAWLHYLEEFGVPQEALRRLVLIREDRPAILAPQGTGCY
DRFFKRSRIWAREITLRLTAVRECFEEVGLLLCRSRSQLDFGAVTCAQAV
PDLESWQRRVHNKPAEFLTLCRELNVVPDLWALHEWSAWASPGFIRKGHE
TVFFMAFVDKQPELLEEPSEVKETLWLTPVELLRLADLGNVWFMPPQVYE
LSRLMGIKAYQSLLEFAIKRSGLGTTMFLPIGYNCQGSMVFVLPGDDFYV
PEPHLVHEIISFPGSEEEFRARSKHLHRYTYGPGVRNLELNIAPPNGHLK
PLKFQAERQKL*

BS28316.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-29 07:50:25
Subject Length Description Subject Range Query Range Score Percent Strand
CG10195-PB 361 CG10195-PB 1..361 1..361 1910 100 Plus
CG10195-PC 361 CG10195-PC 1..361 1..361 1910 100 Plus
CG18094-PB 360 CG18094-PB 8..347 10..349 540 35.3 Plus
CG18094-PA 360 CG18094-PA 8..347 10..349 540 35.3 Plus
CG10194-PA 351 CG10194-PA 7..321 9..328 535 36.3 Plus