Clone BS28411 Report

Search the DGRC for BS28411

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:284
Well:11
Vector:pDNR-Dual
Associated Gene/TranscriptRgk1-RA
Protein status:BS28411.pep: full length peptide match
Sequenced Size:1529

Clone Sequence Records

BS28411.complete Sequence

1529 bp assembled on 2012-04-25

GenBank Submission: KX801389

> BS28411.complete
GAAGTTATCAGTCGACATGGCGCCCTTCTACAAGCGCATCTGCCGCAGTC
TTTCCAAGCGCTACAAGGAGCCCTCGTTAAGTCTGGACAATACAGTCGAG
ACGCTGCTAATCAAGAATGAGGGGGACTTCATCAGCGTGGAGCAGGGCAA
GGAGCTGGTGGCTCGCAAGCGCAACTCCCAGCAGCTGGCACATCCGCCGG
CCCACTTGCATCACCACACCCAGAACCACGCCCACAATCATCACACTCTC
CAGCAGCAGCAGCATCAGCAGCAGCAGCAACAACAACACAAGCGGGACGA
GAATATGAGGCAGCTCCTGGATGTGACCAACACGCTCACCATTGAGGAGC
TGCGCGATTTTCAGATGCGATATGGCTCACCACATCACAGCCGCTCGCAA
TCGGTGAAGATGCCCGGCAGCAGAGCATCCGGAAGGCCAAATCAGCTGTG
TCTGCCCCAGCAGAGATCCAGGGTGGCTTCCATGCCGAATACAGGAGTAG
AGGAGGAGTACTACAGACTGCGTCACTTCTCGATCACCGGCAAGGGTGTG
GTAAATCGCGGTGATTCCCTCAAGAGCCGACGCAGTCGCTCCAACAACAG
CGTGGCCAGTTCCAATTCCAGCACGGAGCACCTGACCACCCAGCAGCAGC
TTTCCGCCCCCGCATCCGTATCCGCTCGAACTTCGCTGGCTTCCAGCAGG
GAGAGCAGTACATCCAATCCGGGCAACGGTCCCTACAGGGTCCTGATGCT
GGGAGGTCCTGCGGTGGGCAAGAGTTCGCTCGTCTCGCAGTTCATGACGT
CGGAGTACCTGCACGCCTACGACACGAGCATCGATGATGAGTCCGGCGAG
AAGGCCGTTTCAGTATTACTCAGTGGCGAAGAGTCCGAGCTGATTTTCAT
CGACCATGGATACACCGAAATGACTCCGGATGAGTGCCTGACCAACTACG
ATCCGCACGGTTACTGCGTCATCTATTCGGCCGCGGACAGGAGCAGCTTC
AGTGTGGCGGAGCAGGTGCTGCAGGTGCTCTGGACCAATCAGAACATCGC
CCAGAAGGCAGTCATTCTAGTATCGAACAAGGCCGACCTGGCCAGGAGTC
GACTGGTCACCTCTGAAGAGGGCAAGGCCATGGCCACCGCCTACGATTGT
AAATTCATCGAGACGTCGGTGGGCATTAATCACAATGTGGACGAGCTGCT
GGTGGGTCTGTTATCACAGATACGACTCAAGCTGGAGAATCCCGAAAAGT
CCAGGGATCTGTTCCGAAAGCGTTCGATTCGAAAGTCAAAGAGACGCGCC
TGCTCGCCACTGAATGCCGGATGCCTGAATGCCAATACCCCTCTGGGTCC
GCTGGGGGAGGCGGTGGCCACGCCCCCGGGCAGTGCCCAAAGCAGTCCCC
GCAAATACCGTGGATCAAGGACCTCCACCAGCCTGAAAGTCAAGGGACTT
TTGGGCCGCGTGTGGACTCGGGACTCCAAGTCGAAGAGCTGCGAGAATCT
GCATGTACTCTAAAAGCTTTCTAGACCAT

BS28411.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-28 12:20:10
Subject Length Description Subject Range Query Range Score Percent Strand
Rgk1-RA 1497 CG44011-PA 1..1497 17..1513 7485 100 Plus
Rgk1-RB 4140 CG44011-PB 2696..4140 69..1513 7225 100 Plus
Rgk1-RC 1545 CG44011-PC 1..819 17..835 4095 100 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-28 12:20:11
Subject Length Description Subject Range Query Range Score Percent Strand
Rgk1-RA 1946 CG44011-RA 246..1742 17..1513 7485 100 Plus
Rgk1-RB 5443 CG44011-RB 3795..5239 69..1513 7225 100 Plus
Rgk1-RC 3239 CG44011-RC 246..1064 17..835 4095 100 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-28 12:20:06
Subject Length Description Subject Range Query Range Score Percent Strand
2R 25286936 2R 19319383..19319688 374..69 1515 99.7 Minus
2R 25286936 2R 19318072..19318341 739..470 1350 100 Minus
2R 25286936 2R 19314561..19314753 1118..926 965 100 Minus
2R 25286936 2R 19312785..19312928 1395..1252 720 100 Minus
2R 25286936 2R 19312994..19313132 1255..1117 695 100 Minus
2R 25286936 2R 19312597..19312718 1513..1392 610 100 Minus
2R 25286936 2R 19318398..19318501 472..369 520 100 Minus
2R 25286936 2R 19317538..19317639 837..736 495 99 Minus
2R 25286936 2R 19315247..19315339 925..833 465 100 Minus
2R 25286936 2R 19325198..19325251 70..17 270 100 Minus
Blast to na_te.dros performed on 2014-11-28 12:20:07 has no hits.

BS28411.complete Sim4 Records

Sim4 to dmel-all-transcript-r5.32.fasta performed 2012-04-25 15:17:53 Download gff for BS28411.complete
Subject Subject Range Query Range Percent Splice Strand
Rgk1-RB 239..1733 17..1511 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 22:41:38 Download gff for BS28411.complete
Subject Subject Range Query Range Percent Splice Strand
Rgk1-RA 246..1740 17..1511 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-28 13:02:07 Download gff for BS28411.complete
Subject Subject Range Query Range Percent Splice Strand
Rgk1-RA 246..1740 17..1511 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-28 13:02:07 Download gff for BS28411.complete
Subject Subject Range Query Range Percent Splice Strand
2R 19319388..19319686 71..369 100 <- Minus
2R 19325198..19325251 17..70 100   Minus
2R 19318073..19318339 472..738 100 <- Minus
2R 19318399..19318500 370..471 100 <- Minus
2R 19312785..19312925 1255..1395 100 <- Minus
2R 19312995..19313130 1119..1254 100 <- Minus
2R 19314561..19314753 926..1118 100 <- Minus
2R 19315247..19315338 834..925 100 <- Minus
2R 19317542..19317636 739..833 100 <- Minus
2R 19312599..19312714 1396..1511 100 <- Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 22:41:38 Download gff for BS28411.complete
Subject Subject Range Query Range Percent Splice Strand
arm_2R 15206893..15207191 71..369 100 <- Minus
arm_2R 15212703..15212756 17..70 100   Minus
arm_2R 15205904..15206005 370..471 100 <- Minus
arm_2R 15200104..15200219 1396..1511 100 <- Minus
arm_2R 15200290..15200430 1255..1395 100 <- Minus
arm_2R 15200500..15200635 1119..1254 100 <- Minus
arm_2R 15202066..15202258 926..1118 100 <- Minus
arm_2R 15202752..15202843 834..925 100 <- Minus
arm_2R 15205047..15205141 739..833 100 <- Minus
arm_2R 15205578..15205844 472..738 100 <- Minus

BS28411.pep Sequence

Translation from 16 to 1512

> BS28411.pep
MAPFYKRICRSLSKRYKEPSLSLDNTVETLLIKNEGDFISVEQGKELVAR
KRNSQQLAHPPAHLHHHTQNHAHNHHTLQQQQHQQQQQQQHKRDENMRQL
LDVTNTLTIEELRDFQMRYGSPHHSRSQSVKMPGSRASGRPNQLCLPQQR
SRVASMPNTGVEEEYYRLRHFSITGKGVVNRGDSLKSRRSRSNNSVASSN
SSTEHLTTQQQLSAPASVSARTSLASSRESSTSNPGNGPYRVLMLGGPAV
GKSSLVSQFMTSEYLHAYDTSIDDESGEKAVSVLLSGEESELIFIDHGYT
EMTPDECLTNYDPHGYCVIYSAADRSSFSVAEQVLQVLWTNQNIAQKAVI
LVSNKADLARSRLVTSEEGKAMATAYDCKFIETSVGINHNVDELLVGLLS
QIRLKLENPEKSRDLFRKRSIRKSKRRACSPLNAGCLNANTPLGPLGEAV
ATPPGSAQSSPRKYRGSRTSTSLKVKGLLGRVWTRDSKSKSCENLHVL*

BS28411.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-29 09:23:42
Subject Length Description Subject Range Query Range Score Percent Strand
Rgk1-PA 498 CG44011-PA 1..498 1..498 2558 100 Plus
Rgk1-PC 514 CG44011-PC 1..514 1..498 2531 96.9 Plus
Rgk1-PB 1379 CG44011-PB 898..1379 17..498 2465 99.6 Plus
Rgk2-PA 740 CG34390-PA 431..740 150..498 692 46.5 Plus
Rgk2-PC 742 CG34390-PC 431..742 150..498 679 46.2 Plus