Clone BS30163 Report

Search the DGRC for BS30163

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:301
Well:63
Vector:pDNR-Dual
Associated Gene/Transcript14-3-3epsilon-RA
Protein status:BS30163.pep: full length peptide match
Sequenced Size:821

Clone Sequence Records

BS30163.complete Sequence

821 bp assembled on 2012-08-22

GenBank Submission: KX806188

> BS30163.complete
GAAGTTATCAGTCGACATGACTGAGCGCGAGAACAATGTGTACAAGGCAA
AGCTGGCCGAACAGGCCGAGCGCTACGACGAAATGGTGGAGGCCATGAAG
AAGGTCGCCTCCATGGACGTAGAGCTGACCGTCGAGGAGCGAAATCTGCT
GTCGGTGGCGTACAAGAATGTGATTGGAGCACGCCGTGCCTCGTGGCGCA
TCATCACCTCGATCGAACAGAAGGAGGAGAACAAGGGGGCCGAGGAGAAA
TTGGAGATGATCAAAACCTACCGCGGACAGGTGGAGAAGGAGCTGCGCGA
CATCTGCTCGGATATACTGAACGTGCTCGAGAAGCATCTCATTCCATGCG
CCACATCCGGCGAAAGCAAAGTATTCTACTATAAGATGAAGGGCGACTAC
CATCGCTACCTGGCCGAATTCGCCACCGGCTCCGACCGCAAGGATGCGGC
AGAGAACTCGCTGATTGCCTACAAGGCGGCCAGCGATATTGCCATGAACG
ATCTGCCACCAACACACCCCATCCGTTTGGGCTTGGCATTGAACTTCTCG
GTGTTCTACTATGAGATTCTCAACTCGCCGGACCGCGCTTGCCGCTTGGC
GAAAGCCGCTTTCGATGATGCCATTGCCGAGTTGGATACACTGAGCGAAG
AGAGCTACAAAGACTCGACACTCATCATGCAGCTGCTGCGCGACAACCTC
ACATTATGGACGTCCGATATGCAGGCAGAAGAAGTAGACCCCAACGCAGG
TGATGGCGAGCCCAAAGAGCAAATCCAGGATGTTGAGGATCAGGACGTGT
CGTAAAAGCTTTCTAGACCAT

BS30163.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-28 12:27:52
Subject Length Description Subject Range Query Range Score Percent Strand
14-3-3epsilon-RA 789 CG31196-PA 1..789 17..805 3945 100 Plus
14-3-3epsilon-RB 786 CG31196-PB 1..786 17..805 3865 99.6 Plus
14-3-3epsilon-RD 783 CG31196-PD 1..783 17..805 3820 99.2 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-28 12:27:53
Subject Length Description Subject Range Query Range Score Percent Strand
14-3-3epsilon-RA 1239 CG31196-RA 160..948 17..805 3945 100 Plus
14-3-3epsilon-RB 3092 CG31196-RB 316..1101 17..805 3865 99.6 Plus
14-3-3epsilon-RD 3360 CG31196-RD 316..1098 17..805 3820 99.2 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-28 12:27:50
Subject Length Description Subject Range Query Range Score Percent Strand
3R 32079331 3R 18247432..18247905 80..553 2370 100 Plus
3R 32079331 3R 18247970..18248151 550..731 910 100 Plus
3R 32079331 3R 18248212..18248288 729..805 385 100 Plus
3R 32079331 3R 18242846..18242909 17..80 320 100 Plus
2R 25286936 2R 10107470..10107576 611..717 280 84.1 Plus
2R 25286936 2R 10106006..10106101 509..604 210 81.2 Plus
2R 25286936 2R 10106354..10106449 509..604 210 81.2 Plus
Blast to na_te.dros performed on 2014-11-28 12:27:51 has no hits.

BS30163.complete Sim4 Records

Sim4 to dmel-all-transcript-r5.32.fasta performed 2012-08-22 17:26:28 Download gff for BS30163.complete
Subject Subject Range Query Range Percent Splice Strand
14-3-3epsilon-RA 316..1102 17..803 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 22:43:32 Download gff for BS30163.complete
Subject Subject Range Query Range Percent Splice Strand
14-3-3epsilon-RA 160..946 17..803 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-28 13:04:47 Download gff for BS30163.complete
Subject Subject Range Query Range Percent Splice Strand
14-3-3epsilon-RA 160..946 17..803 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-28 13:04:47 Download gff for BS30163.complete
Subject Subject Range Query Range Percent Splice Strand
3R 18247971..18248151 551..731 100 -> Plus
3R 18248215..18248286 732..803 100   Plus
3R 18242846..18242909 17..80 100 -> Plus
3R 18247433..18247902 81..550 100 -> Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 22:43:32 Download gff for BS30163.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3R 14068568..14068631 17..80 100 -> Plus
arm_3R 14073155..14073624 81..550 100 -> Plus
arm_3R 14073693..14073873 551..731 100 -> Plus
arm_3R 14073937..14074008 732..803 100   Plus

BS30163.pep Sequence

Translation from 16 to 804

> BS30163.pep
MTERENNVYKAKLAEQAERYDEMVEAMKKVASMDVELTVEERNLLSVAYK
NVIGARRASWRIITSIEQKEENKGAEEKLEMIKTYRGQVEKELRDICSDI
LNVLEKHLIPCATSGESKVFYYKMKGDYHRYLAEFATGSDRKDAAENSLI
AYKAASDIAMNDLPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFD
DAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQAEEVDPNAGDGEPK
EQIQDVEDQDVS*

BS30163.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-29 09:24:21
Subject Length Description Subject Range Query Range Score Percent Strand
14-3-3epsilon-PA 262 CG31196-PA 1..262 1..262 1331 100 Plus
14-3-3epsilon-PB 261 CG31196-PB 1..261 1..262 1314 99.6 Plus
14-3-3epsilon-PD 260 CG31196-PD 1..260 1..262 1309 99.2 Plus
14-3-3epsilon-PC 256 CG31196-PC 1..256 1..262 1282 97.7 Plus
14-3-3zeta-PI 248 CG17870-PI 5..245 3..245 829 67.1 Plus