Clone BS31765 Report

Search the DGRC for BS31765

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:317
Well:65
Vector:pDNR-Dual
Associated Gene/TranscriptE23-RC
Protein status:BS31765.pep: full length peptide match
Sequenced Size:3086

Clone Sequence Records

BS31765.complete Sequence

3086 bp assembled on 2012-09-26

GenBank Submission: KX805980

> BS31765.complete
GAAGTTATCAGTCGACATGATTATTTCCAAGGATCTTTTCATATTTGGCG
AACAAATTTTTGCAACAATCATACCAGAAGGTGGAGCCGCGGCGCTGGCA
ACCCAGCAGCAACATCAGCAGCAGCATCAGCAGCAGCATCAGCAGTCGCA
TCAGCAGCCGCATCAGCAGTCGCAGCAGCAGCAGCAGCAACAACAGCCGG
CAATTATAAATTCAACAACGACGACGGCCACTTCGTTGGTAATAAACTCG
ACAGCAGGAGCAGCTGAACAAGTTGTAGAGGAGTCATTAAAAGTAGCAGC
AGCAGCAGCAGCAGCAACAACAACAACAACAGCCGCAGCAGCAACAACAC
TACCAGCTAAACAGGAAACAGTTGCAGTTGCTGCCGCCTGTGTTGCTCCT
CGCAAATTGGCAAAAATTCAGAAAATTCCAAAGGAACCCGAGGAGCTCAA
AGCCAAGAAAAAGGCAAAGTGTGAGGCAGCAGCAGCGGCGACTCATTACG
AAAAAATGCCAATGAATTTGATGATCGTGGAAAGTGTTGAGGCAGTCGAT
ATGGGATCATCGGACAATGACAACGAGACAACGGAGCAGGTACCGTTGGT
GAGTGCCAGCCGTCGCAATCTGGCCAAAATAGTGGCCGACTGCCGCCTCC
AGATCGAAGTGGAGCCGGCAACATCGGCAGTGGCCGCAGCAGCAGCAGCA
GCAGCAAGCGTTGCATCCCCGCCTGCAACATCGGATGCGACAGCAGCGAC
ATCGGTGGCCAGCAGCGACTCCTCTGCCTCATCATCAGCATCTTCGACGT
CGTCGTCATCTTCCAGCTCAACGTCCTCAGAATTTACGCACACATCACAG
ACAACGATACCAAGTACGGATAATCTAACGTCATCCAATGGTCTGGCCAA
TATAGGCAAAAGCATTTTAGTTGATGCCAAAAGTAACTCCCCAGCTTCCT
TGTCTTACCACTTTGAGAAGTCTGCGGCGGCGAACCAACAGAAGCAGCAT
CACAACATAAACATCTGCAACAGCCACCACCCACATCACCACTCCCAGAA
TCAGCCCCAGCACAGTTTGCAGCTGACCTTCCAGAACCTGAACGTTTTGC
ACAACGAACGCCAAATACTTTCCGATGTGAGTGGATTCGTCAGTCCCTGC
GAGGTCCTGGCCATCATGGGTCCTTCGGGCAGCGGGAAGACCACTCTGCT
GGACTGCCTCAGTGGTCAGCGGCACATTGACAGCGGCAGTGTGTTCCTCA
ACCGTGAGCCCCTGACCAAAAAGTGGCGTCGAAGGATCGGCTATGTCCTC
CAGGAGGAGATCTTCTTCCCGCAATTGACGCTCCGCGAAACGGTGGTCTA
TACTGCGCTGCTGCGACTGCCGGAATCAATGCCGCGGGCGGAGAAGATGC
GCCAGGTGGACCACATACTGGAGGCTCTGGAATTGGGTTGCTGTCAGCAG
ACCAAGTTCGGGGACTACCTCAACCGTGGACTCTCCGGTGGCGAGAAGAA
GCGGGCGAATATCGCCTGTGAACTGCTGACCAATCCGCTGCTCATGCTCC
TGGATGAACCCACTTCTGGACTGGACAGCCACTCGGCCATTTCGCTGATG
AAAGTACTCAAGCGGTACGCCCAGCTGGAGCAGAAGACCATCGTGATCAG
CGTGCACCAGCCTTCGTCCCAGATGTTCCACATGTTCGACAAGCTGCTTC
TGCTCCACCAAGGACGCACTGCGTACTTTGGGGACGTGCAAAACATCTAC
CGCCACTTCGAGGACATCGGGGTCACCATCAAGCCGCACTACAATCCCGC
CGATTTTGTGTTGGAGCAACTGAAATCGCATCCGGATATCCGCGAGAAAC
TCTTCATAGCCGCCAAGGAATCGCACGGCAACTATCTGAACCGCAACTGC
ATCACGAGTAGCCACCATAACCAGGTCAGCGTTTCGGGTGCGAAGGGCAA
GAAGCAAGCGGATAGCATCCTGATCGACGACATAATAAATAACTATTACA
ATCAACGCTCCAACCGGCACCATCACCAGTACGAGAACCTGCACCACACG
AGCAACGGCTGCCGGGTGGAGGAGGACGAGGAGGCGGCCCAGCACCTGGT
GTGGTGTGCCGCGGACTCGCAGTCCAACTTCAGTTCCTGCGCCTCCAGCG
ATTGTCATTCCTATAGTGCTGGCAGTGGGCCAGGACATTCGGCGGATGAC
GACTGGCTGTCCTATCCCACCAGCTTCCACACCCAGTTCTGCGTCCTCTC
AAGTCGCAATTTCCGGGAGGCTAAGCCGCGCATGCTGTCCAAACTCAATT
GGTTCCAAACTATCGGACTGGCTCTGATGGCCGGTGCCATTTGGTTCCAG
CTGCCCAGAACCGAGGAGTTCCTGCACGACCTCCAGGGATGGATGTTCTT
CTCGCAGACCTACTGGATGCTCTTCGCCCTCTTCGGGGCCCTTAATTCAT
TCCCCTCGGAGCGCGAGGTGGTGAGCAAGGAACGTCGCTCCGGGGCCTAC
CGACTATCCGCCTATTATTTGGCCAAAATGTGTGCCGAGCTGCCCTTGGT
GATCACTTTGCCCACGGTCTACCTCATGATCAGCTACCCCATGCTGGGCT
GCACCAGCTTGAAGCTATTCTTCCTGATGCTCATCTTTCTGCTGATCAAC
ACGATCGTGGCCCAGAGCGTGGGCTTCTTCATCGGAGCCTGCTGCATGGA
CATGAACGTGAGCATCACCCTGAGTGCCCTGTACACGCTGGCCACCCAGC
TGTTCGGCGGATATCTGTCTTCGCGCATTCCGGAGGGGCTCAGCTGGATC
CGGTACACCTCCATGATCCACTACGCCTACCAGAACATGCAGATTCTCGA
GTTCCGCGAGGGGGCGGCTATTGGTTGTGGCTCGCCCAGCAGTTACGAGA
TTTGCAAACAGCAATCAACCGAATTCATTCCATACGAGGAAATACTCAAA
GCCCAAAACTCGACATCACCGCTCTGGCTGAACACGCTGATCCTGATGAT
GTTCTTCCTGGTATTCCGGTGCCTGGGATACGCGGTGTTGCGCTTCCTGC
GATGCCCCAAAAAGACGTGAAAGCTTTCTAGACCAT

BS31765.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-28 12:14:58
Subject Length Description Subject Range Query Range Score Percent Strand
E23-RE 3054 CG3327-PE 1..3054 17..3070 15270 100 Plus
E23-RD 3054 CG3327-PD 1..3054 17..3070 15270 100 Plus
E23-RC 3054 CG3327-PC 1..3054 17..3070 15270 100 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-28 12:14:59
Subject Length Description Subject Range Query Range Score Percent Strand
E23-RE 5149 CG3327-RE 1314..4367 17..3070 15270 100 Plus
E23-RD 4397 CG3327-RD 562..3615 17..3070 15270 100 Plus
E23-RC 4514 CG3327-RC 679..3732 17..3070 15270 100 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-28 12:14:56
Subject Length Description Subject Range Query Range Score Percent Strand
2L 23513712 2L 3352476..3353392 933..17 4585 100 Minus
2L 23513712 2L 3336461..3337099 2450..1812 3195 100 Minus
2L 23513712 2L 3337482..3337896 1556..1142 2075 100 Minus
2L 23513712 2L 3335914..3336183 2874..2605 1350 100 Minus
2L 23513712 2L 3337163..3337418 1811..1556 1280 100 Minus
2L 23513712 2L 3338227..3338438 1143..932 1060 100 Minus
2L 23513712 2L 3335657..3335853 3070..2874 985 100 Minus
2L 23513712 2L 3336242..3336398 2607..2451 785 100 Minus
2L 23513712 2L 1135003..1135076 128..201 190 83.8 Plus
Blast to na_te.dros performed 2014-11-28 12:14:57
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2472..2535 669..732 122 65.6 Plus

BS31765.complete Sim4 Records

Sim4 to dmel-all-transcript-r5.32.fasta performed 2012-09-26 11:03:36 Download gff for BS31765.complete
Subject Subject Range Query Range Percent Splice Strand
E23-RC 850..3902 17..3069 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 22:51:45 Download gff for BS31765.complete
Subject Subject Range Query Range Percent Splice Strand
E23-RC 679..3731 17..3069 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-28 13:00:26 Download gff for BS31765.complete
Subject Subject Range Query Range Percent Splice Strand
E23-RC 679..3731 17..3069 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-28 13:00:26 Download gff for BS31765.complete
Subject Subject Range Query Range Percent Splice Strand
2L 3335658..3335852 2875..3069 100 <- Minus
2L 3335914..3336180 2608..2874 100 <- Minus
2L 3336242..3336398 2451..2607 100 <- Minus
2L 3336461..3337099 1812..2450 100 <- Minus
2L 3337163..3337418 1556..1811 100 <- Minus
2L 3337483..3337894 1144..1555 100 <- Minus
2L 3338227..3338436 934..1143 100 <- Minus
2L 3352476..3353392 17..933 100   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 22:51:45 Download gff for BS31765.complete
Subject Subject Range Query Range Percent Splice Strand
arm_2L 3335658..3335852 2875..3069 100 <- Minus
arm_2L 3335914..3336180 2608..2874 100 <- Minus
arm_2L 3336242..3336398 2451..2607 100 <- Minus
arm_2L 3336461..3337099 1812..2450 100 <- Minus
arm_2L 3337163..3337418 1556..1811 100 <- Minus
arm_2L 3337483..3337894 1144..1555 100 <- Minus
arm_2L 3338227..3338436 934..1143 100 <- Minus
arm_2L 3352476..3353392 17..933 100   Minus

BS31765.pep Sequence

Translation from 16 to 3069

> BS31765.pep
MIISKDLFIFGEQIFATIIPEGGAAALATQQQHQQQHQQQHQQSHQQPHQ
QSQQQQQQQQPAIINSTTTTATSLVINSTAGAAEQVVEESLKVAAAAAAA
TTTTTAAAATTLPAKQETVAVAAACVAPRKLAKIQKIPKEPEELKAKKKA
KCEAAAAATHYEKMPMNLMIVESVEAVDMGSSDNDNETTEQVPLVSASRR
NLAKIVADCRLQIEVEPATSAVAAAAAAAASVASPPATSDATAATSVASS
DSSASSSASSTSSSSSSSTSSEFTHTSQTTIPSTDNLTSSNGLANIGKSI
LVDAKSNSPASLSYHFEKSAAANQQKQHHNINICNSHHPHHHSQNQPQHS
LQLTFQNLNVLHNERQILSDVSGFVSPCEVLAIMGPSGSGKTTLLDCLSG
QRHIDSGSVFLNREPLTKKWRRRIGYVLQEEIFFPQLTLRETVVYTALLR
LPESMPRAEKMRQVDHILEALELGCCQQTKFGDYLNRGLSGGEKKRANIA
CELLTNPLLMLLDEPTSGLDSHSAISLMKVLKRYAQLEQKTIVISVHQPS
SQMFHMFDKLLLLHQGRTAYFGDVQNIYRHFEDIGVTIKPHYNPADFVLE
QLKSHPDIREKLFIAAKESHGNYLNRNCITSSHHNQVSVSGAKGKKQADS
ILIDDIINNYYNQRSNRHHHQYENLHHTSNGCRVEEDEEAAQHLVWCAAD
SQSNFSSCASSDCHSYSAGSGPGHSADDDWLSYPTSFHTQFCVLSSRNFR
EAKPRMLSKLNWFQTIGLALMAGAIWFQLPRTEEFLHDLQGWMFFSQTYW
MLFALFGALNSFPSEREVVSKERRSGAYRLSAYYLAKMCAELPLVITLPT
VYLMISYPMLGCTSLKLFFLMLIFLLINTIVAQSVGFFIGACCMDMNVSI
TLSALYTLATQLFGGYLSSRIPEGLSWIRYTSMIHYAYQNMQILEFREGA
AIGCGSPSSYEICKQQSTEFIPYEEILKAQNSTSPLWLNTLILMMFFLVF
RCLGYAVLRFLRCPKKT*

BS31765.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-29 08:49:21
Subject Length Description Subject Range Query Range Score Percent Strand
E23-PE 1017 CG3327-PE 1..1017 1..1017 5236 100 Plus
E23-PD 1017 CG3327-PD 1..1017 1..1017 5236 100 Plus
E23-PC 1017 CG3327-PC 1..1017 1..1017 5236 100 Plus
Atet-PD 832 CG2969-PD 4..826 211..1010 676 26 Plus
Atet-PB 832 CG2969-PB 4..826 211..1010 676 26 Plus