Clone BS32331 Report

Search the DGRC for BS32331

Clone and Library Details

Library:BS
Tissue Source:D. melanogaster
Created by:Joe Carlson
Date Registered:2004-06-03
Comments:Infusion clones with native stop reading frames
Original Plate Number:323
Well:31
Vector:pDNR-Dual
Associated Gene/Transcriptssh-RC
Protein status:BS32331.pep: full length peptide match
Sequenced Size:3173

Clone Sequence Records

BS32331.complete Sequence

3173 bp assembled on 2013-09-16

> BS32331.complete
GAAGTTATCAGTCGACATGAGCATGCAAGTTTGTTCTTTGCCAGACGACA
TCCTTATATTTAGCCTAACCCCACGGGATGGCGAGTCCGAGGACGACGAG
GGCAATAGCAAAGGAAACGATCGCAGCGAGTGCTTCTTTGCCGGCAAGGG
AACCGCCCTGGTACTGGCTCTTAAGGATATCCCGCCCCTGACGCAAAGCG
AGCGTCGCCTGAGCACCGACTCCACCCGCTCCAGTAACAGTACGCAAAGC
AACAACTCGGACATCCAGCTCCACCTGCAATCAATGTTTTACCTGTTGCA
GCGCGAGGACACGCTAAAGATGGCTGTCAAGTTGGAGTCACAGCGGTCCA
ACCGCACGCGCTATCTGGTGATTGCATCGCGTAGTTGTTGTCGGAGCGGC
ACCAGCGACCGTCGGCGCCACCGGATCATGCGTCACCACTCGGTAAAAGT
TGGTGGATCCGCGGGAACAAAGTCGTCCACCTCCCCGGCGGTCCCCACGC
AGCGTCAGCTGTCCGTTGAGCAGACCGCCACAGAGGCCAGCAGCAAATGT
GATAAAACAGCAGACAAGGAAAACGCAACGGCGGCCGGCGACAACAAGAA
TACCTCGGGCATGGAGGAGTCCTGCCTGCTGGGCATAGACTGCAACGAGC
GGACCACCATTGGCCTGGTGGTGCCCATCCTGGCGGACACCACAATTCAC
CTGGATGGCGATGGCGGATTCAGCGTCAAAGTGTACGAAAAAACGCATAT
ATTTAAGCCGGTCTCAGTGCAGGCCATGTGGTCCGCGCTGCAGACGCTGC
ACAAAGTGTCGAAAAAGGCTCGTGAGAACAACTTCTATGCCAGTGGACCC
TCCCACGACTGGCTATCCAGCTACGAGAGGCGCATCGAGTCGGACCAGTC
TTGTCTGAACGAGTGGAATGCCATGGACGCGCTCGAGAGCCGCCGACCAC
CTTCGCCTGATGCCATACGAAACAAACCCCCCGAAAAGGAGGAAACCGAA
AGTGTCATTAAGATGAAGCTGAAGGCCATCATGATGTCAGTGGACCTGGA
CGAGGTCACCTCCAAGTATATACGCGGACGCCTCGAGGAGATCCTGGACA
TGGATTTGGGCGAGTACAAGTCGTTTATCGACGCCGAAATGCTCGTTATT
CTAGGCCAAATGGATGCCCCCACCAAGATTTTCGAGCACGTCTATCTGGG
ATCAGAGTGGAATGCCAGCAACTTGGAGGAGCTGCAGAAAAACGGCGTTC
GCCACATCCTGAACGTGACGCGGGAGATCGATAACTTCTTTCCGGGCACC
TTCGAGTATTTCAACGTTCGCGTGTACGACGATGAGAAGACGAACCTGCT
CAAGTACTGGGACGATACGTTCCGCTACATCACGCGGGCGAAAGCCGAGG
GATCCAAGGTGCTGGTGCACTGCAAGATGGGCGTCAGCCGATCCGCCTCG
GTTGTCATCGCATATGCCATGAAGGCTTACCAGTGGGAGTTCCAGCAGGC
GCTAGAGCACGTGAAGAAGAGGCGCAGCTGTATAAAGCCCAACAAGAACT
TCCTCAATCAGCTGGAGACGTATAGTGGGATGTTGGACGCCATGAAGAAC
AAGGAGAAGCTGCAGCGCAGCAAGAGCGAGACGAACTTGAAGAGCACAAA
GGACGCGCGACTGTTGCCTGGTAGCGAACCAACGCCCCTCATTCAGGCTC
TAAACCAGGCCAAGTCGAAGTCGACTGGTGAAGCGGGCGTAACACCCGAT
GGCGAAGAGGAGGATGGTTCACGCATGCACCGGCGATCCATCGCCCAGAA
GTCACAGCGTCGCATGGTTAGGCGCTCGAGCAGCACATCACCCAAAACCC
AGACGGCTGTGGTTACCAAGCAGCAAAGCCAGTCTATGGAGAACCTTACG
CCGGAACGTAGCGTGGCCGAGGAGCCAAAAAATATGCGCTTCCCAGGCAG
CAACGGCGAGAACTACAGCGTCACCCAGAACCAGGTGCTGCACATCCAGA
AACACACGCCTCTGTCAGTGCGCACACGAATTCACGATCTCGAGGCGCAC
CGGGCGGACCAGCTGCCACAGCAACCTGTCTGGACCTCGCTGACAAAACT
GATTACACAGACCTCTCACTTGGGAAAAAGTGTCAGTGGCAGCAGTAGTG
GAAATATTGACTCGCGGCGGGATTCTAGCTGTTCGGATGTATTCTCCTCG
CAGGTGGACAGTGTTTTTGCCAAGGATGAAGGGGAGAAGCGCCAGCGTCG
AAAGACGCACTCCTGGACAGAGTCCTTGGGTCCAAGTGGCGGCATCGTCC
TGGACCCCACGCCACAGCAACAGAAGCAGCAGTCAAACGCCATTCTGCGT
CCCCGAGGCACACGTCAGCGGGAGCTGCCTTCGCGGCACGCAAGTTGGGG
ATCAGGAGACAACCGATGCTGTCTGCCGCAGAGGACCAGTTCCGGCTCCT
ACTATGACTCCAATCGCAACACTACGGCCATCTTCGAGGGCGTTATCCAA
GACCTCAAGCGCAGTTCCAATTGCAATGTAATCGAAGGAGTCGCCGTTGC
TGTGGAACCGATAGTTGGAGTTGGCGAGGGCACCGTAAAGCGCACCAAGC
AGAAGCTGGAAGAGAGCACCTCGCTGAAAAAGCGCTGCCAGGAAGAAAGC
CAGGAGCTGCTACTCGAGGCTGTGGATGCTGGGCAGAGGCGGTGCCCAAG
CCTCTATAGGAGCGCCTCCTCGGCGCACAGTCCGCGGCAGCGTCAGCCAC
TACGCTGTAATAGTGAGGAGCTGATGCACACCAGCGACATCGAGAACAAG
AATACCACGCCAGGCGACCATGAGGCGACGGTAGTGCTGCGCGTCCAGGG
ACAAACGCTGGCCGACGATCAGCGCATCTCGGGCAATGTGCAAATACTCA
AGCAAAATTTCGAAGCCAAAGCGGGAGTGGTGGGCACAGGAGGAGGAGGA
GGCGGTGGCACTGCAGCGGGATCGGGCAGTAGTTCAACAATCGCAACGTC
GGTATCGGCGGTGGCCGGAGCCTGCTCAGCGAATCCCGGCAAGAAGACGA
CTAGCCACCAATCACTGCCATCGTCGCCGGTGGCGCAGCATGCCAACCAC
GTGTCCGCCGCCTCCAACTCCAACTCCTCCGCCTCCAACTCGTCCGATTC
CAGCTAGAAGCTTTCTAGACCAT

BS32331.complete Blast Records

Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-28 13:47:40
Subject Length Description Subject Range Query Range Score Percent Strand
ssh-RC 3141 CG6238-PC 1..3141 17..3157 15690 100 Plus
ssh-RB 3582 CG6238-PB 1..3138 17..3154 15675 100 Plus
ssh-RD 3138 CG6238-PD 57..3138 76..3157 15395 100 Plus
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-28 13:47:41
Subject Length Description Subject Range Query Range Score Percent Strand
ssh-RC 4186 CG6238-RC 247..3387 17..3157 15690 100 Plus
ssh-RB 4254 CG6238-RB 247..3384 17..3154 15675 100 Plus
ssh-RD 4419 CG6238-RD 539..3620 76..3157 15395 100 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-28 13:47:37
Subject Length Description Subject Range Query Range Score Percent Strand
3R 32079331 3R 24939873..24941782 3154..1245 9535 99.9 Minus
3R 32079331 3R 24942819..24943211 714..322 1965 100 Minus
3R 32079331 3R 24941867..24942142 1245..970 1365 99.6 Minus
3R 32079331 3R 24943275..24943522 323..76 1240 100 Minus
3R 32079331 3R 24942203..24942398 975..780 980 100 Minus
3R 32079331 3R 24942459..24942527 782..714 345 100 Minus
3R 32079331 3R 24943589..24943649 77..17 305 100 Minus
Blast to na_te.dros performed on 2014-11-28 13:47:38 has no hits.

BS32331.complete Sim4 Records

Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-09-16 11:49:22 Download gff for BS32331.complete
Subject Subject Range Query Range Percent Splice Strand
ssh-RC 247..3387 17..3157 99   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-28 14:35:14 Download gff for BS32331.complete
Subject Subject Range Query Range Percent Splice Strand
ssh-RC 247..3387 17..3157 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-28 14:35:14 Download gff for BS32331.complete
Subject Subject Range Query Range Percent Splice Strand
3R 24939868..24941781 1246..3157 99 <- Minus
3R 24941867..24942136 976..1245 100 <- Minus
3R 24942203..24942397 781..975 100 <- Minus
3R 24942461..24942526 715..780 100 <- Minus
3R 24942819..24943210 323..714 100 <- Minus
3R 24943276..24943521 77..322 100 <- Minus
3R 24943590..24943649 17..76 100   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-09-16 11:49:22 Download gff for BS32331.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3R 20765590..20767503 1246..3157 99 <- Minus
arm_3R 20767589..20767858 976..1245 100 <- Minus
arm_3R 20767925..20768119 781..975 100 <- Minus
arm_3R 20768183..20768248 715..780 100 <- Minus
arm_3R 20768541..20768932 323..714 100 <- Minus
arm_3R 20768998..20769243 77..322 100 <- Minus
arm_3R 20769312..20769371 17..76 100   Minus

BS32331.pep Sequence

Translation from 16 to 3156

> BS32331.pep
MSMQVCSLPDDILIFSLTPRDGESEDDEGNSKGNDRSECFFAGKGTALVL
ALKDIPPLTQSERRLSTDSTRSSNSTQSNNSDIQLHLQSMFYLLQREDTL
KMAVKLESQRSNRTRYLVIASRSCCRSGTSDRRRHRIMRHHSVKVGGSAG
TKSSTSPAVPTQRQLSVEQTATEASSKCDKTADKENATAAGDNKNTSGME
ESCLLGIDCNERTTIGLVVPILADTTIHLDGDGGFSVKVYEKTHIFKPVS
VQAMWSALQTLHKVSKKARENNFYASGPSHDWLSSYERRIESDQSCLNEW
NAMDALESRRPPSPDAIRNKPPEKEETESVIKMKLKAIMMSVDLDEVTSK
YIRGRLEEILDMDLGEYKSFIDAEMLVILGQMDAPTKIFEHVYLGSEWNA
SNLEELQKNGVRHILNVTREIDNFFPGTFEYFNVRVYDDEKTNLLKYWDD
TFRYITRAKAEGSKVLVHCKMGVSRSASVVIAYAMKAYQWEFQQALEHVK
KRRSCIKPNKNFLNQLETYSGMLDAMKNKEKLQRSKSETNLKSTKDARLL
PGSEPTPLIQALNQAKSKSTGEAGVTPDGEEEDGSRMHRRSIAQKSQRRM
VRRSSSTSPKTQTAVVTKQQSQSMENLTPERSVAEEPKNMRFPGSNGENY
SVTQNQVLHIQKHTPLSVRTRIHDLEAHRADQLPQQPVWTSLTKLITQTS
HLGKSVSGSSSGNIDSRRDSSCSDVFSSQVDSVFAKDEGEKRQRRKTHSW
TESLGPSGGIVLDPTPQQQKQQSNAILRPRGTRQRELPSRHASWGSGDNR
CCLPQRTSSGSYYDSNRNTTAIFEGVIQDLKRSSNCNVIEGVAVAVEPIV
GVGEGTVKRTKQKLEESTSLKKRCQEESQELLLEAVDAGQRRCPSLYRSA
SSAHSPRQRQPLRCNSEELMHTSDIENKNTTPGDHEATVVLRVQGQTLAD
DQRISGNVQILKQNFEAKAGVVGTGGGGGGGTAAGSGSSSTIATSVSAVA
GACSANPGKKTTSHQSLPSSPVAQHANHVSAASNSNSSASNSSDSS*

BS32331.pep Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-29 09:40:58
Subject Length Description Subject Range Query Range Score Percent Strand
ssh-PC 1046 CG6238-PC 1..1046 1..1046 5366 100 Plus
ssh-PB 1193 CG6238-PB 1..1046 1..1046 5366 100 Plus
ssh-PD 1045 CG6238-PD 15..1045 16..1046 5265 99.6 Plus
ssh-PA 1192 CG6238-PA 15..1045 16..1046 5265 99.6 Plus
Mkp3-PA 241 CG14080-PA 43..186 383..523 202 31.2 Plus