Clone FI20172 Report

Search the DGRC for FI20172

Clone and Library Details

Library:FI
Tissue Source:various Drosophila melanogaster
Created by: 
Date Registered:2005-11-08
Comments:Drosophila melanogaster corrected cDNA clones
Original Plate Number:201
Well:72
Vector:pFlc-1
Associated Gene/Transcripttey-RA
Protein status:FI20172.pep: gold
Sequenced Size:2842

Clone Sequence Records

FI20172.complete Sequence

2842 bp assembled on 2012-04-17

GenBank Submission: BT133456.1

> FI20172.complete
AGTTGGATTTCTGCAGCGCGTACGTGCGGGTGTCCTGTGTCGAGCGAGCG
AGAGAGTTTTAGAGAGAAGGAGAGCGAGCGAGCTGTCAGCTGATCCGAGC
TTTACCGTTTTACCGACGCGCAAATACAATTCCCAACCGATTATCACCGA
TACCGAGTGATCTTTGCATGTGCCAACATTGAAACACAAGCGAAAGTGAG
GTTATGAGTGCCCCCTAGTGCCAAATTAGTCTAAACCAAAACCAAAACAA
AACACAAACGAGCTGCAGATGGAAAGCAACGCATTTGGCAGCAGCCATTT
GCCGTCCCAAGCGCTCGTTGTCCTCTCGGAAGCCGCTTCCGGTCTGCACG
AGGCTCTACGTGGACAACGTCCGTTTCCATCGAGAGTAATCCCTTTCGAT
TTCCCCCTCTTGCAGCTACCCGACGCCAAAGATCTGCACAATATGTCACT
AGTCGGCAACTACTTCAATCCGCACCTGCTGCTCAACCACGGGATGCTGG
TGGCCAACGGAGCGGCGGCGGCAGCGGCGGCGGCGGGAGCGGGACCGGCC
AGCTACTTTGCCAGTGACCGCTCGCCGCTGGGCAAGCCGTCGGTGCTGTC
CAACTTCTCGCTGCCGTCGGCCTTCTCGCCGCCCAAGTACATCGGCATAT
CCCTGGATCAGAATCTGTTCAATGGCAGCGAATCATTTCGCACGGATTCG
GCCAGCCCCACATGCACATCGCACGAATCCATGGAGGGATCACAGGATTA
CGATGCCGTTGAAAAGGGTGAAAGTCCGCGCAGCAATAATTCTCAGGATC
CCAGGGATTTGAGACATCTGCATAATGCGGGGAAGAGCCACCAGGCACTG
GCCACTTCCTCGTCGGCGTCCAGCTCCTCGTCCTCCTCCTGCTCCACCAG
CAATCCCGCCATCAGCACGGCGACCAGCAGCGTTGCCGTTTCGATGGCCA
GCCACCTGGCCGCCTCCTCGCCGCACCATCACCCCCACACGCACGCCCAC
TCGCACCCGCATCCGTTGGCCCATCCGCACGCCCATAGCCATCACCACGT
GGGCCACCATGTGGGGGCACCTCCGGTGTCCACGGCGGTGACGACGCACC
ACCACATGGCCCATCCCCATCCGCTGTCGCACCACCACGCCGCCCACCAC
GCGGCCTTGGCCAGCTTGAGCATGGCGGGTCTGCGAGCAGTTCCCGGCGG
ATTGAGCCTGGTCAGTGGGCTGCAGGCGGCAGCTGCCGGCGGTGCTATTC
CCGAGATGTGCCCCGTTTGCGGACTGAAGCTGAGCGCCGAGGAGTGGCAC
ACCCACTTCCTCACCGAACTGGACAGGCTCTACAAGCTGAGTGCGGGATT
CGAGAGGGCCAGTCTGCAGGCCACCTACATGTTTGCTCCTCCATGTCCCG
CGCAGGAGAACGCCATTCGCACCAGTCACAACCGCTGGGAGACCTTTCAG
AGAATACGTAACAATCGCCAGAACCGACTGAGACTCAAAGTGCGAAAGCG
CAAGTACGGCGAAATGTACATGATGGAGAGTCTTTACTGTAGCAGTTGTC
CGATCTGCAAGAGGAAGTACGCCTTGGAGACGGGGAAGATTCCTCCGGAG
GACGATGCCAAATCGCAGGAGGAGATCGAAACGGTGGATGTGGAGAGCTG
CAACGACGAAGTGCCCGACTCAGGTTCGGAGCTGGCTACTCCGAGTTCTG
GGTCATCCGGCACGGTTATGCCGCCCTCCAGTATGCACAACAGCAACTCG
CAGCCGGGCAAGCTGGACGGGATTCTCTACCGGACGGGCTGTGTGCTCAG
CCAGAAGGATCCGCATCCCGACGAGCATGACGTGAGCACCAATGTGACAA
CGGCGAGCAGCAGTAGTTGGCCGGGAGAAGCACCAACCCAGGCACATATC
AGTGTTAAAACCGTCAGCGAGCTGTCATCCACCACACACCACTACTACAA
CGCGGACTCATGCGGCGTGGGCGTAAACGACACCAACAGCAGCAGCAGCA
CCCACAACAACGGCGGTGGCAGCAACAAGGATCTGATGATGGACACGGCC
TCCTGCCAAAACGACAGCGACGAGGACGTGATTGTGGACGACGACGACAC
CGTGAAGCTGACGAGCAAAATCAACTATGGCAAGATTCAGCGCCGCCAGG
ACGAGCAGAACGTCGGCAGCAGCAGATCCCTCGAGAACATCTCACCGGTG
GAGGAGCGACCGCGGTCGGAGCCGCAGGTGAGCAGCACCGAACCCGGACC
CATGGACATATCTCACAACAACAACAATAACAACAACAACAACAACAACA
ATAGCAACAGTAATAACAACAACAATCCGTCGACGAAATACGCAGAGAGC
ATGGAGAACAGCCTGTCGCAGTTGTCGTCGATGGGCGTGCCGGGATTAAC
GCAATTGGACACAAAGGCTTAAAGGATCTCAACACGGATGCGTTTCTGCG
CGGCCGTAACTTTTACTTCCACCAGAGTTGTGCCAACGTCATGAGCAGCT
ACCGGCTGAACAAGGAGCTTCTCAGCCAGGCCCAGAATCAACAGAGGGCG
GCCGCCAACGGAAACGGAAATGGCAGTGGGATCGGAATTGGGGATTCGGG
CGGCGCCACGGCAACGCCCCGCAGCCTTTCACCTGCCCAATCTCCGCAGC
AGAGCGTTTCGCAGGCCATGGAGTGAGCGGAGGAGTGGATGGGATAATAG
CAATCAAAGCGTGAATTTATTAAAATTAATTAAGAACATATTGTATTCGA
GGTAGCTAATGAAACGCGACGAGCCGAAGTGGTTAATGGGGCATCCCCAA
AGTAGGCAACACCAGCCACGCCCACCAAAAAAAAAAAAAAAA

FI20172.complete Blast Records

Blast to d_melanogaster_OreR.fa performed 2019-03-15 13:44:38
Subject Length Description Subject Range Query Range Score Percent Strand
chr3L 24539361 chr3L 19642635..19643260 1441..816 3115 99.8 Minus
chr3L 24539361 chr3L 19640163..19640574 2826..2415 2000 99 Minus
chr3L 24539361 chr3L 19652613..19653001 389..1 1915 99.5 Minus
chr3L 24539361 chr3L 19641992..19642297 1904..1599 1500 99.3 Minus
chr3L 24539361 chr3L 19646443..19646718 661..386 1380 100 Minus
chr3L 24539361 chr3L 19641493..19641764 2175..1904 1360 100 Minus
chr3L 24539361 chr3L 19640815..19641059 2417..2173 1210 99.6 Minus
chr3L 24539361 chr3L 19642412..19642573 1601..1440 810 100 Minus
chr3L 24539361 chr3L 19646157..19646313 815..659 785 100 Minus
Blast to na_all.dmel.RELEASE6 performed 2019-03-15 13:44:35
Subject Length Description Subject Range Query Range Score Percent Strand
3L 28110227 3L 19653290..19653915 1441..816 3130 100 Minus
3L 28110227 3L 19650802..19651229 2842..2415 2140 100 Minus
3L 28110227 3L 19663275..19663663 389..1 1945 100 Minus
3L 28110227 3L 19652647..19652952 1904..1599 1515 99.7 Minus
3L 28110227 3L 19657096..19657371 661..386 1380 100 Minus
3L 28110227 3L 19652148..19652419 2175..1904 1360 100 Minus
3L 28110227 3L 19651470..19651714 2417..2173 1225 100 Minus
3L 28110227 3L 19653067..19653228 1601..1440 810 100 Minus
3L 28110227 3L 19656810..19656966 815..659 785 100 Minus
Blast to na_te.dros performed 2019-03-15 13:44:37
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2319..2659 1981..2324 244 56.4 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6867..7198 1982..2325 212 56.7 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2368..2838 1856..2323 205 53.3 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2750..2987 2097..2325 203 59.9 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6533..6876 1978..2324 197 55.7 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6744..7091 1976..2323 173 54.1 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2335..2716 1976..2362 168 53.2 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2436..2492 1972..2028 159 75.4 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2444..2494 1977..2027 156 78.4 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6728..7092 1981..2364 153 54.4 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2398..2450 1976..2028 148 75.5 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2787..3026 1981..2227 148 56.7 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6765..6816 1976..2027 143 75 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2830..3011 1976..2148 140 57.9 Plus
roo 9092 roo DM_ROO 9092bp 1125..1188 1976..2040 133 69.2 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2361..2666 1981..2295 128 54.8 Plus
roo 9092 roo DM_ROO 9092bp 1066..1123 1971..2028 128 69 Plus
roo 9092 roo DM_ROO 9092bp 1119..1157 1976..2014 123 79.5 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 1530..1599 1963..2031 122 65.7 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6803..6849 1981..2027 118 72.3 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 1488..1586 1918..2016 117 57.6 Plus
roo 9092 roo DM_ROO 9092bp 1121..1165 1981..2025 117 73.3 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6786..6837 1976..2027 116 69.2 Plus

FI20172.complete Sim4 Records

Sim4 to d_melanogaster_OreR.fa performed 2019-03-15 13:45:32 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
chr3L 19640163..19640572 2417..2826 99 <- Minus
chr3L 19640816..19641056 2176..2416 99 <- Minus
chr3L 19641493..19641763 1905..2175 100 <- Minus
chr3L 19641992..19642295 1601..1904 99 <- Minus
chr3L 19642413..19642571 1442..1600 100 <- Minus
chr3L 19642635..19643260 816..1441 99 <- Minus
chr3L 19646157..19646310 662..815 100 <- Minus
chr3L 19646443..19646718 386..661 100 <- Minus
chr3L 19652617..19653001 1..385 99   Minus
Sim4 to dmel-all-CDS-r5.32.fasta performed 2012-04-17 17:25:30 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
tey-RA 1..2154 269..2422 99   Plus
Sim4 to dmel-all-CDS-r5.52.fasta performed 2013-08-03 22:11:28 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
tey-RC 1..117 269..385 100 == Plus
tey-RC 118..2382 416..2676 99   Plus
Sim4 to dmel-all-CDS-r6.02.fasta performed 2014-11-26 20:40:09 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
tey-RC 1..117 269..385 100 == Plus
tey-RC 118..2382 416..2676 99   Plus
Sim4 to dmel-all-transcript-r5.32.fasta performed 2012-04-17 17:25:29 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
tey-RA 1..2826 1..2826 99   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-03 22:11:28 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
tey-RA 2..2827 1..2826 99   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-26 20:40:09 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
tey-RA 2..2827 1..2826 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-15 13:45:32 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
3L 19650818..19651227 2417..2826 100 <- Minus
3L 19651471..19651711 2176..2416 100 <- Minus
3L 19652148..19652418 1905..2175 100 <- Minus
3L 19652647..19652950 1601..1904 99 <- Minus
3L 19653068..19653226 1442..1600 100 <- Minus
3L 19653290..19653915 816..1441 100 <- Minus
3L 19656810..19656963 662..815 100 <- Minus
3L 19657096..19657371 386..661 100 <- Minus
3L 19663279..19663663 1..385 100   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-15 13:45:32 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
3L 19650818..19651227 2417..2826 100 <- Minus
3L 19651471..19651711 2176..2416 100 <- Minus
3L 19652148..19652418 1905..2175 100 <- Minus
3L 19652647..19652950 1601..1904 99 <- Minus
3L 19653068..19653226 1442..1600 100 <- Minus
3L 19653290..19653915 816..1441 100 <- Minus
3L 19656810..19656963 662..815 100 <- Minus
3L 19657096..19657371 386..661 100 <- Minus
3L 19663279..19663663 1..385 100   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-15 13:45:32 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
3L 19650818..19651227 2417..2826 100 <- Minus
3L 19651471..19651711 2176..2416 100 <- Minus
3L 19652148..19652418 1905..2175 100 <- Minus
3L 19652647..19652950 1601..1904 99 <- Minus
3L 19653068..19653226 1442..1600 100 <- Minus
3L 19653290..19653915 816..1441 100 <- Minus
3L 19656810..19656963 662..815 100 <- Minus
3L 19657096..19657371 386..661 100 <- Minus
3L 19663279..19663663 1..385 100   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-03 22:11:28 Download gff for FI20172.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3L 19643918..19644327 2417..2826 100 <- Minus
arm_3L 19644571..19644811 2176..2416 100 <- Minus
arm_3L 19645248..19645518 1905..2175 100 <- Minus
arm_3L 19645747..19646050 1601..1904 99 <- Minus
arm_3L 19646168..19646326 1442..1600 100 <- Minus
arm_3L 19646390..19647015 816..1441 100 <- Minus
arm_3L 19649910..19650063 662..815 100 <- Minus
arm_3L 19650196..19650471 386..661 100 <- Minus
arm_3L 19656379..19656763 1..385 100   Minus

FI20172.hyp Sequence

Translation from 268 to 2421

> FI20172.hyp
MESNAFGSSHLPSQALVVLSEAASGLHEALRGQRPFPSRVIPFDFPLLQL
PDAKDLHNMSLVGNYFNPHLLLNHGMLVANGAAAAAAAAGAGPASYFASD
RSPLGKPSVLSNFSLPSAFSPPKYIGISLDQNLFNGSESFRTDSASPTCT
SHESMEGSQDYDAVEKGESPRSNNSQDPRDLRHLHNAGKSHQALATSSSA
SSSSSSSCSTSNPAISTATSSVAVSMASHLAASSPHHHPHTHAHSHPHPL
AHPHAHSHHHVGHHVGAPPVSTAVTTHHHMAHPHPLSHHHAAHHAALASL
SMAGLRAVPGGLSLVSGLQAAAAGGAIPEMCPVCGLKLSAEEWHTHFLTE
LDRLYKLSAGFERASLQATYMFAPPCPAQENAIRTSHNRWETFQRIRNNR
QNRLRLKVRKRKYGEMYMMESLYCSSCPICKRKYALETGKIPPEDDAKSQ
EEIETVDVESCNDEVPDSGSELATPSSGSSGTVMPPSSMHNSNSQPGKLD
GILYRTGCVLSQKDPHPDEHDVSTNVTTASSSSWPGEAPTQAHISVKTVS
ELSSTTHHYYNADSCGVGVNDTNSSSSTHNNGGGSNKDLMMDTASCQNDS
DEDVIVDDDDTVKLTSKINYGKIQRRQDEQNVGSSRSLENISPVEERPRS
EPQVSSTEPGPMDISHNNNNNNNNNNNNSNSNNNNNPSTKYAESMENSLS
QLSSMGVPGLTQLDTKA*

FI20172.hyp Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 17:28:41
Subject Length Description Subject Range Query Range Score Percent Strand
tey-PA 717 CG8780-PA 1..717 1..717 3785 100 Plus
tey-PB 822 CG8780-PB 1..716 1..716 3781 100 Plus
tey-PC 793 CG8780-PC 1..706 1..716 3707 98.6 Plus
tey-PD 745 CG8780-PD 1..658 59..716 3484 100 Plus
Vsx2-PB 645 CG33980-PB 49..439 135..579 166 25.1 Plus

FI20172.pep Sequence

Translation from 268 to 2421

> FI20172.pep
MESNAFGSSHLPSQALVVLSEAASGLHEALRGQRPFPSRVIPFDFPLLQL
PDAKDLHNMSLVGNYFNPHLLLNHGMLVANGAAAAAAAAGAGPASYFASD
RSPLGKPSVLSNFSLPSAFSPPKYIGISLDQNLFNGSESFRTDSASPTCT
SHESMEGSQDYDAVEKGESPRSNNSQDPRDLRHLHNAGKSHQALATSSSA
SSSSSSSCSTSNPAISTATSSVAVSMASHLAASSPHHHPHTHAHSHPHPL
AHPHAHSHHHVGHHVGAPPVSTAVTTHHHMAHPHPLSHHHAAHHAALASL
SMAGLRAVPGGLSLVSGLQAAAAGGAIPEMCPVCGLKLSAEEWHTHFLTE
LDRLYKLSAGFERASLQATYMFAPPCPAQENAIRTSHNRWETFQRIRNNR
QNRLRLKVRKRKYGEMYMMESLYCSSCPICKRKYALETGKIPPEDDAKSQ
EEIETVDVESCNDEVPDSGSELATPSSGSSGTVMPPSSMHNSNSQPGKLD
GILYRTGCVLSQKDPHPDEHDVSTNVTTASSSSWPGEAPTQAHISVKTVS
ELSSTTHHYYNADSCGVGVNDTNSSSSTHNNGGGSNKDLMMDTASCQNDS
DEDVIVDDDDTVKLTSKINYGKIQRRQDEQNVGSSRSLENISPVEERPRS
EPQVSSTEPGPMDISHNNNNNNNNNNNNSNSNNNNNPSTKYAESMENSLS
QLSSMGVPGLTQLDTKA*

FI20172.pep Blast Records

Blast to dana-all-translation-r1.3.fasta performed 2019-03-17 00:33:56
Subject Length Description Subject Range Query Range Score Percent Strand
Dana\Om(2D)-PA 813 GF10000-PA 1..715 1..717 2695 87.4 Plus
Blast to dere-all-translation-r1.3.fasta performed 2019-03-17 00:33:57
Subject Length Description Subject Range Query Range Score Percent Strand
Dere\GG13384-PA 797 GG13384-PA 1..706 1..716 2910 93.6 Plus
Blast to dgri-all-translation-r1.3.fasta performed 2019-03-17 00:34:00
Subject Length Description Subject Range Query Range Score Percent Strand
Dgri\GH14618-PA 784 GH14618-PA 286..694 304..717 1485 77.6 Plus
Dgri\GH12962-PA 86 GH12962-PA 1..40 1..40 192 92.5 Plus
Blast to dmel-all-translation-r6.23.fasta performed 2019-03-25 10:29:44
Subject Length Description Subject Range Query Range Score Percent Strand
tey-PA 717 CG8780-PA 1..717 1..717 3785 100 Plus
tey-PB 822 CG8780-PB 1..716 1..716 3781 100 Plus
tey-PC 793 CG8780-PC 1..706 1..716 3707 98.6 Plus
tey-PD 745 CG8780-PD 1..658 59..716 3484 100 Plus
Vsx2-PB 645 CG33980-PB 49..439 135..579 166 25.1 Plus
rdx-PE 829 CG12537-PE 154..377 80..301 165 26.2 Plus
stx-PB 1122 CG32676-PB 260..384 186..322 162 33.3 Plus
stx-PA 1122 CG32676-PA 260..384 186..322 162 33.3 Plus
Vsx2-PA 640 CG33980-PA 49..258 135..370 159 26.5 Plus
Blast to dmoj-all-translation-r1.3.fasta performed 2019-03-17 00:34:02
Subject Length Description Subject Range Query Range Score Percent Strand
Dmoj\GI13505-PA 775 GI13505-PA 289..721 285..717 1570 80.1 Plus
Blast to dper-all-translation-r1.3.fasta performed 2019-03-17 00:34:04
Subject Length Description Subject Range Query Range Score Percent Strand
Dper\GL20875-PA 826 GL20875-PA 1..680 1..666 2284 79.6 Plus
Blast to dpse-all-translation-r3.2.fasta performed 2019-03-17 00:34:06
Subject Length Description Subject Range Query Range Score Percent Strand
Dpse\GA21316-PA 823 GA21316-PA 1..726 1..716 2402 79.7 Plus
Blast to dsec-all-translation-r1.3.fasta performed 2019-03-17 00:34:07
Subject Length Description Subject Range Query Range Score Percent Strand
Dsec\GM16921-PA 748 GM16921-PA 1..661 1..716 3353 90.4 Plus
Blast to dsim-all-translation-r1.4.fasta performed 2019-03-17 00:34:09
Subject Length Description Subject Range Query Range Score Percent Strand
Dsim\GD12258-PA 636 GD12258-PA 1..636 1..636 3349 98.7 Plus
Dsim\GD15560-PA 56 GD15560-PA 1..40 1..40 201 100 Plus
Blast to dvir-all-translation-r1.2.fasta performed 2019-03-17 00:34:11
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\GJ11866-PA 771 GJ11866-PA 288..697 304..716 1483 79.4 Plus
Blast to dwil-all-translation-r1.3.fasta performed 2019-03-17 00:34:13
Subject Length Description Subject Range Query Range Score Percent Strand
Dwil\GK25781-PA 828 GK25781-PA 1..736 1..716 2080 71.8 Plus
Blast to dyak-all-translation-r1.3.fasta performed 2019-03-17 00:34:15
Subject Length Description Subject Range Query Range Score Percent Strand
Dyak\GE22476-PA 814 GE22476-PA 1..717 1..716 3686 97.1 Plus
Dyak\GE22765-PA 833 GE22765-PA 1..736 1..716 3661 94.4 Plus