Clone GH13849 Report

Search the DGRC for GH13849

Clone and Library Details

Library:GH
Tissue Source:Drosophila melanogaster head
Created by:Ling Hong
Date Registered:1998-06-02
Comments:Sized fractionated cDNAs were directly ligated into pOT2. Plasmid cDNA library.
Original Plate Number:138
Well:49
Vector:pOT2
Associated Gene/TranscriptS-Lap2-RA
Protein status:GH13849.pep: gold
Sequenced Size:2017

Associated Genes

Associations are from manual ordering of a clone or by a periodic analysis.
Gene Date Evidence
CG32351 2008-04-29 Release 5.5 accounting
CG32351 2008-04-29 Picked prior to 5.5
CG32351 2008-08-15 Release 5.9 accounting
CG32351 2008-12-18 5.12 accounting

Clone Sequence Records

GH13849.complete Sequence

2017 bp assembled on 2008-06-05

GenBank Submission: BT033046

> GH13849.complete
TTGAACACGTACACACTTTTAGCTAGTCTGGGTGAAAAAATTTCATGAAT
TATTTTGAAGCGGCACAGAAAATCGAAGAGAAAGTTTTCAATACTAGTAG
TAATATTATTCAGCATTCATCAAAGTTTTAAGTTAAACTTCAAGCAAAAT
GTTTCGGTTTTCTCGCAATGCCATAACCCGGGCGTGTAGTCTCTCCATTC
GTCGGCCGGAGGTGATCCGTCATCGCAGGTACGCCTCGCAGGCGATCAAC
CAGATGCTGCAGCTGCAGCAGATGGAGATCTGCGCGGATCCTCCGTCGCG
TGGCTTGGTCGTTGGAGTCTACGCCGACGAGGAGGACAAGAACGACGCTG
GCATCCTGACGCCAACCGGCTGGAAGTACAATGTGCAGAAGACGCACGGT
CGGTTGCTAGAAGTGCTGCGAATGTCCGGACCCATGCCCAAAAGGGGAGA
GACCCGCCTCCTGTTCGCCGTGGAGCCGGAGCGCGTGCCCTACTATTCGG
CGGTGGCAGTTATCGGTTTGGGTAAGGAATGCCTGGGCTATAACCCATAC
GAGGTGCTCGACGAGCAGAAGGAGGCCATCCGACGGTCGGTAGCGGATGC
CTGCCGAATACTCGCTGAACTGGACACCGACCGCATCGAAGTAGAGAACT
GTGGACACGCCGAATCTGCCGCGGAGGGAGCAGCTCTCGGGATCTGGATC
TACCAGGAGCTTCGCGATCCCAAGCGGCGAATCTCAGTGCCTTCCATCGA
TCTGTATGCCACTAAGGATGAGATTTGCGACATAGAAGGATGGCGTATCG
GCCTGCAAAAGGCTGCCGCGCAGAACCTGACCCGCCAGTTGCAGGAGATG
CCCTCCAACCTTTTGACCCCCACTGCCTTTGCCCAGAATGTCGTCGAAGT
GCTTTGTAAGTCCGGCGTCAATGTAGAGGTCAAAGTCGAGGGTTGGGCTG
AGAGCCAGTCGATGCACGCCTTCTTGGCGGTGGGAAAGGCCTCTTGCGAA
CCACCAATCTTTCTGGAGCTGAGCTACTACGGCACCTGTGCCGAGGAGCG
ACCCATCGTCTTGGTTGGGCAGGGAGTGACTTACGATGCAGGTGGTTTGT
GCTTAAAGGAGAAGCAGGAACTTTTCCACATGCGCGGAGATATGACTGGT
GCGGCCGTAGTGGTGGCCACTTGTCGTGCTGTCGCTGGCCTGAGGTTACC
GGTCAATATTCGTGGACTAATACCCCTGTGCGAGAACGTTATTGGATGCA
ATTCCTTCCGTCCTGGAGACATGGTGAAATCCATGAATGGCAAGACCATT
GAGGTGCAGTGCACCGACCACGAAGATGTTCTAGTGCTGGCGGATGCCCT
TCTGTACGGGCAGAACTTCTGTCCGAAGTGCATCATTGACATTGGCACCT
GCTCGGGATATATGCGCCAAGCGTTGGATGAGGCTGCCTGTGGGGTGTTT
ACCAACTCTGAGATCCTTTGGCAGCAGATCAAGCACGCCAGCATGCACAC
TGGAGACCGCGTGTGGCGTTTCCCCCTGTGGAACTACTACAGCAAGGCGG
TGCGTGCCGGTGGACGCAGTGATGTCCAGAACTACGGTATCGGTCGTGGT
GGACGTCCTTGCAAAGCTGCCGCTTTCCTTCGTGAATTTGTGCCTTGCGG
CCAATGGATGCATATTGATGCCACCAACGTGATGATCACACGTGGTGATG
AGTTCGAGTATCTGCGAGAGGGCATGGCCGGACGTCCAACAAGGACACTC
ATCGAGTTTATTGCACAGTCCATATGCAAGGACACAGCTCCAAAGCTTAA
CAAGTGAACCTTCGAACGGTGTTTAAGTTTAATGAGACGATGCCACACGT
TCATCGGTATTCTTTTAACGACTGACCAACCTATGACTTCTTTAACGTTC
TGACGTTTATCTAATTGGGACCACGAAATGTTAAAATTAATTCGTTTATT
AATGACATACAAATTATAAGTCATGTAAAAAAGTGAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAA

GH13849.complete Blast Records

Blast to MB8.fasta performed 2010-07-15 21:03:27
Subject Length Description Subject Range Query Range Score Percent Strand
CG32351-RA 2093 CG32351-RA 109..2093 1..1985 9925 100 Plus
CG6372-RA 2228 CG6372-RA 400..1965 230..1795 4230 84.6 Plus
CG5978-RA 1562 CG5978-RA 1506..1562 1989..1933 285 100 Minus
Blast to d_melanogaster_OreR.fa performed 2019-03-16 20:34:09
Subject Length Description Subject Range Query Range Score Percent Strand
chr3L 24539361 chr3L 8612021..8613223 1..1203 6015 100 Plus
chr3L 24539361 chr3L 8609552..8610510 230..1188 2590 84.7 Plus
chr3L 24539361 chr3L 8613276..8613742 1201..1667 2335 100 Plus
chr3L 24539361 chr3L 8613798..8614116 1667..1985 1595 100 Plus
chr3L 24539361 chr3L 8610580..8611046 1201..1667 1480 87.8 Plus
Blast to dmel-all-all_noncoding-r5.12.fasta performed on 2010-04-22 16:50:02 has no hits.
Blast to na_all.dmel.RELEASE6 performed 2019-03-16 20:34:06
Subject Length Description Subject Range Query Range Score Percent Strand
3L 28110227 3L 8619996..8621198 1..1203 6015 100 Plus
3L 28110227 3L 8617527..8618485 230..1188 2590 84.7 Plus
3L 28110227 3L 8621251..8621717 1201..1667 2335 100 Plus
3L 28110227 3L 8621773..8622095 1667..1989 1615 100 Plus
3L 28110227 3L 8618555..8619021 1201..1667 1480 87.8 Plus
Blast to na_arms.dmel.RELEASE6 performed 2011-12-12 22:21:55
Subject Length Description Subject Range Query Range Score Percent Strand
3L 28103327 3L 8613096..8614298 1..1203 6015 100 Plus
3L 28103327 3L 8610627..8611585 230..1188 2590 84.6 Plus
3L 28103327 3L 8614351..8614817 1201..1667 2335 100 Plus
3L 28103327 3L 8614873..8615195 1667..1989 1615 100 Plus
3L 28103327 3L 8611655..8612121 1201..1667 1480 87.7 Plus
3L 28103327 3L 8612183..8612311 1667..1795 180 75.9 Plus
Blast to na_te.dros performed 2019-03-16 20:34:07
Subject Length Description Subject Range Query Range Score Percent Strand
HeT-A 6083 HeT-A DM06920 6083bp Derived from U06920.2 (Rel. 67, Last updated, Version 14). 340..389 1979..1931 130 76 Minus

GH13849.complete Sim4 Records

Sim4 to d_melanogaster_OreR.fa performed 2019-03-16 20:35:05 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
chr3L 8613798..8614116 1667..1985 100   Plus
chr3L 8612021..8613221 1..1201 100 -> Plus
chr3L 8613277..8613741 1202..1666 100 -> Plus
Sim4 to dmel-all-CDS-r5.12.fasta performed 2008-12-08 16:40:58 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
CG32351-RA 1..1659 149..1807 100   Plus
Sim4 to dmel-all-CDS-r5.32.fasta performed 2011-03-16 21:04:58 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
CG32351-RA 1..1659 149..1807 100   Plus
Sim4 to dmel-all-CDS-r5.52.fasta performed 2013-08-04 17:10:58 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
S-Lap2-RA 1..1659 149..1807 100   Plus
Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-22 00:52:15 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
CG32351-RA 1..1659 149..1807 100   Plus
Sim4 to dmel-all-CDS-r6.02.fasta performed 2014-11-27 16:07:09 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
S-Lap2-RA 1..1659 149..1807 100   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2008-11-11 01:27:42 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
CG32351-RA 109..2093 1..1985 100   Plus
Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-03-16 21:04:58 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
CG32351-RA 109..2093 1..1985 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 17:10:58 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
S-Lap2-RA 109..2093 1..1985 100   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-22 00:52:15 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
CG32351-RA 109..2093 1..1985 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-27 16:07:09 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
S-Lap2-RA 109..2093 1..1985 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 20:35:05 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
3L 8619996..8621196 1..1201 100 -> Plus
3L 8621252..8621716 1202..1666 100 -> Plus
3L 8621773..8622091 1667..1985 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 20:35:05 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
3L 8619996..8621196 1..1201 100 -> Plus
3L 8621252..8621716 1202..1666 100 -> Plus
3L 8621773..8622091 1667..1985 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 20:35:05 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
3L 8619996..8621196 1..1201 100 -> Plus
3L 8621252..8621716 1202..1666 100 -> Plus
3L 8621773..8622091 1667..1985 100   Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 17:10:58 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3L 8613096..8614296 1..1201 100 -> Plus
arm_3L 8614352..8614816 1202..1666 100 -> Plus
arm_3L 8614873..8615191 1667..1985 100   Plus
Sim4 to na_arms.dmel.RELEASE6 performed 2011-12-09 18:33:27 Download gff for GH13849.complete
Subject Subject Range Query Range Percent Splice Strand
3L 8613096..8614296 1..1201 100 -> Plus
3L 8614352..8614816 1202..1666 100 -> Plus
3L 8614873..8615191 1667..1985 100   Plus

GH13849.pep Sequence

Translation from 148 to 1806

> GH13849.pep
MFRFSRNAITRACSLSIRRPEVIRHRRYASQAINQMLQLQQMEICADPPS
RGLVVGVYADEEDKNDAGILTPTGWKYNVQKTHGRLLEVLRMSGPMPKRG
ETRLLFAVEPERVPYYSAVAVIGLGKECLGYNPYEVLDEQKEAIRRSVAD
ACRILAELDTDRIEVENCGHAESAAEGAALGIWIYQELRDPKRRISVPSI
DLYATKDEICDIEGWRIGLQKAAAQNLTRQLQEMPSNLLTPTAFAQNVVE
VLCKSGVNVEVKVEGWAESQSMHAFLAVGKASCEPPIFLELSYYGTCAEE
RPIVLVGQGVTYDAGGLCLKEKQELFHMRGDMTGAAVVVATCRAVAGLRL
PVNIRGLIPLCENVIGCNSFRPGDMVKSMNGKTIEVQCTDHEDVLVLADA
LLYGQNFCPKCIIDIGTCSGYMRQALDEAACGVFTNSEILWQQIKHASMH
TGDRVWRFPLWNYYSKAVRAGGRSDVQNYGIGRGGRPCKAAAFLREFVPC
GQWMHIDATNVMITRGDEFEYLREGMAGRPTRTLIEFIAQSICKDTAPKL
NK*

GH13849.pep Blast Records

Blast to dana-all-translation-r1.3.fasta performed 2019-03-15 17:51:59
Subject Length Description Subject Range Query Range Score Percent Strand
Dana\GF24339-PA 553 GF24339-PA 1..553 1..552 2868 96 Plus
Dana\GF24338-PA 555 GF24338-PA 1..555 1..552 2698 90.5 Plus
Dana\GF11165-PA 529 GF11165-PA 41..527 50..545 983 40.2 Plus
Dana\GF10596-PA 522 GF10596-PA 34..517 51..543 959 39.4 Plus
Dana\GF10595-PA 535 GF10595-PA 5..532 15..545 952 38.8 Plus
Blast to dere-all-translation-r1.3.fasta performed 2019-03-15 17:52:00
Subject Length Description Subject Range Query Range Score Percent Strand
Dere\GG14332-PA 552 GG14332-PA 1..552 1..552 2944 98.9 Plus
Dere\GG14331-PA 555 GG14331-PA 1..555 1..552 2694 89.7 Plus
Dere\GG20395-PA 552 GG20395-PA 2..551 8..546 975 40.3 Plus
Dere\GG20609-PA 534 GG20609-PA 9..534 16..544 966 39.8 Plus
Dere\GG15437-PA 523 GG15437-PA 34..518 50..543 948 39.4 Plus
Blast to dgri-all-translation-r1.3.fasta performed 2019-03-15 17:52:00
Subject Length Description Subject Range Query Range Score Percent Strand
Dgri\GH22523-PA 547 GH22523-PA 1..547 1..552 2740 91.8 Plus
Dgri\GH22522-PA 546 GH22522-PA 4..546 9..552 2656 89.7 Plus
Dgri\GH20870-PA 532 GH20870-PA 47..530 53..545 990 40.7 Plus
Dgri\GH14946-PA 528 GH14946-PA 42..525 53..545 955 39.2 Plus
Dgri\GH21028-PA 554 GH21028-PA 67..553 51..546 948 41.5 Plus
Blast to dmel-all-translation-r6.23.fasta performed 2019-03-25 10:24:39
Subject Length Description Subject Range Query Range Score Percent Strand
S-Lap2-PA 552 CG32351-PA 1..552 1..552 2913 100 Plus
S-Lap1-PA 555 CG6372-PA 1..555 1..552 2637 89.1 Plus
S-Lap8-PA 534 CG4439-PA 50..534 51..544 985 41.2 Plus
S-Lap5-PA 549 CG18369-PA 61..548 51..546 985 42.1 Plus
S-Lap3-PD 535 CG32063-PD 47..532 51..545 950 39.7 Plus
S-Lap3-PC 535 CG32063-PC 47..532 51..545 950 39.7 Plus
S-Lap3-PB 535 CG32063-PB 47..532 51..545 950 39.7 Plus
S-Lap4-PA 524 CG32064-PA 35..519 50..543 949 39.8 Plus
S-Lap7-PB 527 CG13340-PB 39..525 50..545 941 38.9 Plus
S-Lap7-PA 527 CG13340-PA 39..525 50..545 941 38.9 Plus
loopin-1-PC 526 CG4750-PC 37..524 45..543 913 40.4 Plus
loopin-1-PA 526 CG4750-PA 37..524 45..543 913 40.4 Plus
grsm-PB 558 CG7340-PB 221..508 224..507 261 29.1 Plus
grsm-PF 655 CG7340-PF 318..605 224..507 261 29.1 Plus
Blast to dmoj-all-translation-r1.3.fasta performed 2019-03-15 17:52:01
Subject Length Description Subject Range Query Range Score Percent Strand
Dmoj\GI12915-PA 549 GI12915-PA 1..549 1..552 2740 91.5 Plus
Dmoj\GI12914-PA 546 GI12914-PA 1..546 1..552 2672 89.7 Plus
Dmoj\GI18629-PA 551 GI18629-PA 64..550 51..546 998 41.3 Plus
Dmoj\GI20526-PA 525 GI20526-PA 20..525 30..544 974 38.7 Plus
Dmoj\GI11471-PA 526 GI11471-PA 17..525 18..547 959 38.2 Plus
Blast to dper-all-translation-r1.3.fasta performed 2019-03-15 17:52:01
Subject Length Description Subject Range Query Range Score Percent Strand
Dper\GL10324-PA 555 GL10324-PA 1..555 1..552 2649 87.3 Plus
Dper\GL24718-PA 552 GL24718-PA 1..552 1..552 2634 87.5 Plus
Dper\GL15275-PA 552 GL15275-PA 1..552 1..552 2631 87.3 Plus
Dper\GL13211-PA 552 GL13211-PA 1..552 1..552 2625 87.3 Plus
Dper\GL15615-PA 536 GL15615-PA 1..536 1..552 2493 84.4 Plus
Blast to dpse-all-translation-r3.2.fasta performed 2019-03-15 17:52:02
Subject Length Description Subject Range Query Range Score Percent Strand
Dpse\GA19547-PA 563 GA19547-PA 9..563 1..552 2659 87.9 Plus
Dpse\GA27986-PA 553 GA27986-PA 2..553 1..552 2630 87.3 Plus
Dpse\GA27985-PA 517 GA27985-PA 1..517 36..552 2564 90.3 Plus
Dpse\GA27987-PA 943 GA27987-PA 2..499 1..498 2356 87.8 Plus
Dpse\GA27987-PA 943 GA27987-PA 504..943 101..552 2103 87.6 Plus
Dpse\GA23253-PA 717 GA23253-PA 328..717 141..552 1769 81.6 Plus
Blast to dsec-all-translation-r1.3.fasta performed 2019-03-15 17:52:02
Subject Length Description Subject Range Query Range Score Percent Strand
Dsec\GM25074-PA 555 GM25074-PA 1..555 1..552 2683 89.7 Plus
Dsec\GM25075-PA 397 GM25075-PA 1..351 1..351 1860 99.7 Plus
Dsec\GM25212-PA 524 GM25212-PA 34..519 49..543 961 39.4 Plus
Dsec\GM21704-PA 534 GM21704-PA 9..534 16..544 958 39.4 Plus
Dsec\GM21706-PA 526 GM21706-PA 30..524 25..543 931 39.8 Plus
Dsec\GM25075-PA 397 GM25075-PA 342..397 488..552 246 80 Plus
Blast to dsim-all-translation-r1.4.fasta performed 2019-03-15 17:52:03
Subject Length Description Subject Range Query Range Score Percent Strand
Dsim\GD14113-PA 552 GD14113-PA 1..552 1..552 2962 99.8 Plus
Dsim\GD14112-PA 547 GD14112-PA 1..547 1..552 2414 83 Plus
Dsim\GD10979-PA 549 GD10979-PA 2..548 8..546 965 39.8 Plus
Dsim\GD25742-PA 527 GD25742-PA 39..525 50..545 954 39.1 Plus
Dsim\GD11203-PA 526 GD11203-PA 30..524 25..543 931 39.8 Plus
Blast to dvir-all-translation-r1.2.fasta performed 2019-03-15 17:52:04
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\GJ13059-PA 547 GJ13059-PA 1..546 1..551 2726 91.8 Plus
Dvir\GJ13057-PA 545 GJ13057-PA 1..545 1..552 2673 89.5 Plus
Dvir\GJ21638-PA 553 GJ21638-PA 73..552 58..546 974 41.3 Plus
Dvir\GJ20697-PA 523 GJ20697-PA 42..521 57..545 959 38.8 Plus
Dvir\GJ13666-PA 521 GJ13666-PA 33..520 50..546 946 37.8 Plus
Blast to dwil-all-translation-r1.3.fasta performed 2019-03-15 17:52:04
Subject Length Description Subject Range Query Range Score Percent Strand
Dwil\GK13104-PA 548 GK13104-PA 1..548 1..552 2784 93.5 Plus
Dwil\GK13093-PA 546 GK13093-PA 1..546 1..552 2692 89.7 Plus
Dwil\GK21700-PA 539 GK21700-PA 19..537 3..545 993 39 Plus
Dwil\GK17945-PA 527 GK17945-PA 4..526 17..546 971 38.1 Plus
Dwil\GK15975-PA 551 GK15975-PA 64..550 51..546 963 42.1 Plus
Blast to dyak-all-translation-r1.3.fasta performed 2019-03-15 17:52:05
Subject Length Description Subject Range Query Range Score Percent Strand
Dyak\GE20760-PA 552 GE20760-PA 1..552 1..552 2955 99.5 Plus
Dyak\GE20759-PA 555 GE20759-PA 1..555 1..552 2691 89.6 Plus
Dyak\GE21747-PA 523 GE21747-PA 34..518 50..543 959 39.8 Plus
Dyak\GE13342-PA 527 GE13342-PA 39..525 50..545 959 39.3 Plus
Dyak\GE12557-PA 552 GE12557-PA 2..551 8..546 959 39.6 Plus

GH13849.hyp Sequence

Translation from 148 to 1806

> GH13849.hyp
MFRFSRNAITRACSLSIRRPEVIRHRRYASQAINQMLQLQQMEICADPPS
RGLVVGVYADEEDKNDAGILTPTGWKYNVQKTHGRLLEVLRMSGPMPKRG
ETRLLFAVEPERVPYYSAVAVIGLGKECLGYNPYEVLDEQKEAIRRSVAD
ACRILAELDTDRIEVENCGHAESAAEGAALGIWIYQELRDPKRRISVPSI
DLYATKDEICDIEGWRIGLQKAAAQNLTRQLQEMPSNLLTPTAFAQNVVE
VLCKSGVNVEVKVEGWAESQSMHAFLAVGKASCEPPIFLELSYYGTCAEE
RPIVLVGQGVTYDAGGLCLKEKQELFHMRGDMTGAAVVVATCRAVAGLRL
PVNIRGLIPLCENVIGCNSFRPGDMVKSMNGKTIEVQCTDHEDVLVLADA
LLYGQNFCPKCIIDIGTCSGYMRQALDEAACGVFTNSEILWQQIKHASMH
TGDRVWRFPLWNYYSKAVRAGGRSDVQNYGIGRGGRPCKAAAFLREFVPC
GQWMHIDATNVMITRGDEFEYLREGMAGRPTRTLIEFIAQSICKDTAPKL
NK*

GH13849.hyp Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 07:45:25
Subject Length Description Subject Range Query Range Score Percent Strand
S-Lap2-PA 552 CG32351-PA 1..552 1..552 2913 100 Plus
S-Lap1-PA 555 CG6372-PA 1..555 1..552 2637 89.1 Plus
S-Lap8-PA 534 CG4439-PA 50..534 51..544 985 41.2 Plus
S-Lap5-PA 549 CG18369-PA 61..548 51..546 985 42.1 Plus
S-Lap3-PD 535 CG32063-PD 47..532 51..545 950 39.7 Plus