Clone GM13874 Report

Search the DGRC for GM13874

Clone and Library Details

Library:GM
Tissue Source:Drosophila melanogaster ovary
Created by:Ling Hong
Date Registered:1997-11-24
Comments:Constructed using Stratagene ZAP-cDNA Synthesis kit. Oligo dT-primed and directionally cloned at EcoRI and XhoI in BlueScript SK(+/-)
Original Plate Number:138
Well:74
Vector:pOT2
Associated Gene/TranscriptRac2-RA
Protein status:GM13874.pep: gold
Preliminary Size:1300
Sequenced Size:1263

Associated Genes

Associations are from manual ordering of a clone or by a periodic analysis.
Gene Date Evidence
CG8556 2001-01-01 Release 2 assignment
CG8556 2002-05-16 Blastp of sequenced clone
CG8556 2003-01-01 Sim4 clustering to Release 3
Rac2 2008-04-29 Release 5.5 accounting
Rac2 2008-08-15 Release 5.9 accounting
Rac2 2008-12-18 5.12 accounting

Clone Sequence Records

GM13874.complete Sequence

1263 bp (1263 high quality bases) assembled on 2002-05-16

GenBank Submission: AY118845

> GM13874.complete
CCACAGAAAATCAGCAAATCCCCACATATGCGGGCCCAATTAATTCATAA
ATAATATACCGACCGAACAGAAAGAACGCCGTCACGGTTCTGCTTTCAAT
TAAGAATATTGATTTGACAACTTTCGCGATAAATCAAACTCCACTTCAAA
TTCGCATCTCTCTCTGTACGCGATTGCTTGAATGTGTGTGTGTGTGTGGG
CAGAATTTGTATTAGTGCGTGAGCAAATTATTGTATAATCGCCAAAAAAA
AAAAAAACAATAAAAGCAAAACGCCAACGCGAAAAGCAAAAGCTATACAA
TTAGAAAAACTAGCTGAGCTGCGATACAAGGCAAAACTAAATTGTGTGAC
TAAAATTTGTCTGTTGAACTAAAAGAAAAGTACAAAATAGTACAAAAGTA
CAAATATCGAAACATTCAAAAACTGTCTGACCTACTGAATTGAATGAATT
ACCCCAAAGTAATTATAAGTTAAGCTGCCACAAAGAAAACCAGTTGAGCC
ACTGGAAATCGAGCACGAAACCAGCTGATCCACTGAAAAACCCTCTGCCC
CCAGCGCACAAGCAACCCATCGAGCTGCTGAACCCATCGATTGCCCAGTA
GTACCACCAGTACAGTAGCATTCATCATGCAGGCCATCAAGTGTGTGGTT
GTGGGCGACGGAGCGGTGGGAAAGACCTGCCTGCTGATCAGCTATACGAC
CAACGCCTTCCCCGGCGAGTACATACCCACGGTGTTCGACAACTATTCGG
CGAATGTGATGGTGGATGCCAAGCCCATCAATCTGGGCCTCTGGGATACG
GCTGGACAGGAGGACTACGATCGCCTGAGGCCGCTATCCTATCCGCAAAC
GGATGTCTTTCTCATCTGTTTCTCACTGGTGAATCCGGCATCGTTTGAGA
ATGTGCGAGCCAAATGGTTTCCCGAGGTGCGTCATCATTGCCCGAGTGTG
CCGATAATCCTGGTCGGCACCAAACTGGATCTGCGCGACGATAAGCAGAC
GATCGAGAAGCTGAAGGACAAGAAGCTAACACCGATCACCTATCCCCAGG
GACTGGCGATGGCCAAGGAAATAGCTGCGGTCAAGTATCTGGAGTGCTCG
GCCCTGACCCAAAAGGGTCTGAAGACGGTCTTCGACGAGGCCATACGATC
CGTGCTATGTCCTGTCGTTCGAGGACCCAAGCGGCACAAGTGCGCCCTGC
TCTAAGAACTATGAACCCACCCAAAATAAAAAAGCGAAGAACTTCAAAAA
AAAAAAAAAAAAA

GM13874.complete Blast Records

Blast to MB8.fasta performed 2010-07-15 19:07:48
Subject Length Description Subject Range Query Range Score Percent Strand
Rac2-RA 1288 Rac2-RA 43..1288 1..1245 5980 98.8 Plus
Rac1-RA 1805 Rac1-RA 337..855 626..1144 1575 86.8 Plus
Cdc42-RB 1528 Cdc42-RB 285..420 626..761 290 80.8 Plus
Cdc42-RB 1528 Cdc42-RB 442..518 783..859 250 88.3 Plus
Cdc42-RB 1528 Cdc42-RB 739..803 1080..1144 175 84.6 Plus
Blast to d_melanogaster_OreR.fa performed 2019-03-15 18:01:58
Subject Length Description Subject Range Query Range Score Percent Strand
chr3L 24539361 chr3L 7425992..7427237 1245..1 5885 98.5 Minus
chr3L 24539361 chr3L 1301335..1301853 1144..626 1575 86.9 Minus
chrX 22417052 chrX 19586734..19586967 626..859 465 79.9 Plus
chr3R 27901430 chr3R 23461031..23461095 783..847 220 89.2 Plus
Blast to dmel-all-all_noncoding-r5.12.fasta performed on 2010-04-22 17:22:51 has no hits.
Blast to na_all.dmel.RELEASE6 performed 2019-03-15 18:01:56
Subject Length Description Subject Range Query Range Score Percent Strand
3L 28110227 3L 7433874..7435137 1263..1 6020 98.7 Minus
3L 28110227 3L 1301829..1302347 1144..626 1575 86.9 Minus
X 23542271 X 19697957..19698190 626..859 480 80.3 Plus
3R 32079331 3R 27638122..27638186 783..847 220 89.2 Plus
Blast to na_arms.dmel.RELEASE6 performed 2011-12-12 20:38:50
Subject Length Description Subject Range Query Range Score Percent Strand
3L 28103327 3L 7426974..7428237 1263..1 6040 98.7 Minus
3L 28103327 3L 1301829..1302347 1144..626 1575 86.8 Minus
X 23527363 X 19706055..19706190 626..761 290 80.8 Plus
X 23527363 X 19706212..19706288 783..859 250 88.3 Plus
3R 31820162 3R 27378953..27379017 783..847 220 89.2 Plus
X 23527363 X 19706570..19706634 1080..1144 175 84.6 Plus
3R 31820162 3R 27378735..27378836 634..735 150 76.4 Plus
Blast to na_te.dros performed on 2019-03-15 18:01:56 has no hits.

GM13874.complete Sim4 Records

Sim4 to d_melanogaster_OreR.fa performed 2019-03-15 18:02:41 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
chr3L 7426016..7427237 1..1221 98   Minus
Sim4 to dmel-all-CDS-r5.12.fasta performed 2008-12-08 17:08:01 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
Rac2-RA 1..579 627..1205 99   Plus
Sim4 to dmel-all-CDS-r5.32.fasta performed 2011-03-16 17:51:03 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
Rac2-RA 1..579 627..1205 99   Plus
Sim4 to dmel-all-CDS-r5.52.fasta performed 2013-08-04 02:53:04 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
Rac2-RA 1..579 627..1205 99   Plus
Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-21 18:43:39 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
Rac2-RA 1..579 627..1205 99   Plus
Sim4 to dmel-all-CDS-r6.02.fasta performed 2014-11-26 21:53:03 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
Rac2-RA 1..579 627..1205 99   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2008-11-10 21:28:37 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
Rac2-RA 43..1288 1..1245 98   Plus
Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-03-16 17:51:02 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
Rac2-RA 43..1288 1..1245 98   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 02:53:04 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
Rac2-RA 54..1299 1..1245 98   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-21 18:43:40 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
Rac2-RA 43..1288 1..1245 98   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-26 21:53:03 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
Rac2-RA 54..1299 1..1245 98   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-15 18:02:41 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
3L 7433892..7435137 1..1245 98   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-15 18:02:41 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
3L 7433892..7435137 1..1245 98   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-15 18:02:41 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
3L 7433892..7435137 1..1245 98   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 02:53:04 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3L 7426992..7428237 1..1245 98   Minus
Sim4 to na_arms.dmel.RELEASE6 performed 2011-12-09 15:15:57 Download gff for GM13874.complete
Subject Subject Range Query Range Percent Splice Strand
3L 7426992..7428237 1..1245 98   Minus

GM13874.pep Sequence

Translation from 626 to 1204

> GM13874.pep
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDAKP
INLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVNPASFENVRAKWFPE
VRHHCPSVPIILVGTKLDLRDDKQTIEKLKDKKLTPITYPQGLAMAKEIA
AVKYLECSALTQKGLKTVFDEAIRSVLCPVVRGPKRHKCALL*

GM13874.pep Blast Records

Blast to dana-all-translation-r1.3.fasta performed 2019-03-15 23:31:45
Subject Length Description Subject Range Query Range Score Percent Strand
Dana\GF23788-PA 192 GF23788-PA 1..192 1..192 1006 97.9 Plus
Dana\GF10067-PA 192 GF10067-PA 1..192 1..192 954 92.7 Plus
Dana\GF16294-PA 195 GF16294-PA 7..195 4..192 711 66.1 Plus
Dana\GF20423-PA 191 GF20423-PA 1..191 1..192 710 67.7 Plus
Dana\GF11290-PA 192 GF11290-PA 7..192 5..192 561 56.4 Plus
Blast to dere-all-translation-r1.3.fasta performed 2019-03-15 23:31:46
Subject Length Description Subject Range Query Range Score Percent Strand
Dere\GG14963-PA 192 GG14963-PA 1..192 1..192 1022 100 Plus
Dere\GG14611-PA 192 GG14611-PA 1..192 1..192 959 93.2 Plus
Dere\GG19248-PA 191 GG19248-PA 1..191 1..192 712 68.2 Plus
Dere\GG11563-PA 195 GG11563-PA 7..195 4..192 711 66.1 Plus
Dere\GG20566-PA 192 GG20566-PA 7..192 5..192 561 56.4 Plus
Blast to dgri-all-translation-r1.3.fasta performed 2019-03-15 23:31:46
Subject Length Description Subject Range Query Range Score Percent Strand
Dgri\GH16006-PA 192 GH16006-PA 1..192 1..192 955 92.7 Plus
Dgri\GH16613-PA 192 GH16613-PA 1..192 1..192 954 94.3 Plus
Dgri\GH19274-PA 195 GH19274-PA 7..195 4..192 720 66.7 Plus
Dgri\GH12491-PA 191 GH12491-PA 1..191 1..192 712 68.8 Plus
Dgri\GH22067-PA 192 GH22067-PA 7..192 5..192 562 56.9 Plus
Blast to dmel-all-translation-r6.23.fasta performed 2019-03-25 10:13:21
Subject Length Description Subject Range Query Range Score Percent Strand
Rac2-PB 192 CG8556-PB 1..192 1..192 1012 100 Plus
Rac2-PA 192 CG8556-PA 1..192 1..192 1012 100 Plus
Rac1-PB 192 CG2248-PB 1..192 1..192 949 93.2 Plus
Rac1-PA 192 CG2248-PA 1..192 1..192 949 93.2 Plus
Mtl-PC 195 CG5588-PC 7..195 4..192 707 66.1 Plus
Mtl-PA 195 CG5588-PA 7..195 4..192 707 66.1 Plus
Mtl-PB 195 CG5588-PB 7..195 4..192 707 66.1 Plus
Cdc42-PD 191 CG12530-PD 1..191 1..192 702 68.2 Plus
Cdc42-PC 191 CG12530-PC 1..191 1..192 702 68.2 Plus
Cdc42-PA 191 CG12530-PA 1..191 1..192 702 68.2 Plus
Rho1-PA 192 CG8416-PA 7..192 5..192 551 56.4 Plus
Rho1-PG 192 CG8416-PG 7..192 5..192 551 56.4 Plus
Rho1-PF 192 CG8416-PF 7..192 5..192 551 56.4 Plus
Rho1-PD 192 CG8416-PD 7..192 5..192 551 56.4 Plus
Rho1-PC 192 CG8416-PC 7..192 5..192 551 56.4 Plus
Rho1-PB 192 CG8416-PB 7..192 5..192 551 56.4 Plus
RhoL-PA 190 CG9366-PA 12..175 4..176 502 55.5 Plus
RhoL-PB 214 CG9366-PB 36..199 4..176 502 55.5 Plus
RhoU-PC 582 CG34104-PC 392..570 3..184 382 45.6 Plus
RhoU-PB 582 CG34104-PB 392..570 3..184 382 45.6 Plus
RhoBTB-PC 738 CG5701-PC 12..211 2..177 282 36.9 Plus
RhoBTB-PA 745 CG5701-PA 12..211 2..177 282 36.9 Plus
RhoBTB-PB 783 CG5701-PB 12..211 2..177 282 36.9 Plus
Rala-PB 197 CG2849-PB 13..172 5..176 212 31.8 Plus
Rala-PC 201 CG2849-PC 13..172 5..176 209 31.2 Plus
Rala-PA 201 CG2849-PA 13..172 5..176 209 31.2 Plus
Ras85D-PA 189 CG9375-PA 1..163 1..176 206 31.5 Plus
Ras64B-PA 192 CG1167-PA 3..166 1..176 200 29.7 Plus
Rab35-PB 201 CG9575-PB 10..131 5..126 199 34.1 Plus
Rab35-PD 201 CG9575-PD 10..131 5..126 199 34.1 Plus
Rab35-PA 201 CG9575-PA 10..131 5..126 199 34.1 Plus
Rab21-PC 222 CG17515-PC 15..169 5..170 199 29 Plus
Rab21-PD 222 CG17515-PD 15..169 5..170 199 29 Plus
Rab23-PA 268 CG2108-PA 37..187 3..146 198 29.6 Plus
Rab10-PA 204 CG17060-PA 11..171 5..177 194 29.1 Plus
Rap2l-PA 182 CG3204-PA 1..177 1..189 184 27.2 Plus
Ran-PD 216 CG1404-PD 12..130 5..123 183 33.3 Plus
Ran-PC 216 CG1404-PC 12..130 5..123 183 33.3 Plus
Ran-PA 216 CG1404-PA 12..130 5..123 183 33.3 Plus
Ran-PB 216 CG1404-PB 12..130 5..123 183 33.3 Plus
RabX2-PA 195 CG2885-PA 9..129 5..124 182 32 Plus
Rab1-PA 205 CG3320-PA 13..132 5..123 182 33.1 Plus
Rab3-PB 220 CG7576-PB 23..183 5..178 181 26.7 Plus
Rab3-PA 220 CG7576-PA 23..183 5..178 181 26.7 Plus
Rab19-PB 219 CG7062-PP3-RB 23..143 5..124 180 35.2 Plus
Rab19-PA 219 CG7062-PA 23..143 5..124 180 35.2 Plus
Rab6-PB 208 CG6601-PB 10..166 1..169 179 31 Plus
Rab6-PA 208 CG6601-PA 10..166 1..169 179 31 Plus
CG13375-PC 277 CG13375-PC 20..181 5..173 177 26.3 Plus
Rab35-PC 190 CG9575-PC 8..120 14..126 176 34.2 Plus
Rab9Fb-PA 214 CG32670-PA 9..129 5..124 176 35.2 Plus
Blast to dmoj-all-translation-r1.3.fasta performed 2019-03-15 23:31:47
Subject Length Description Subject Range Query Range Score Percent Strand
Dmoj\GI12458-PA 192 GI12458-PA 1..192 1..192 968 94.3 Plus
Dmoj\GI12273-PA 192 GI12273-PA 1..192 1..192 959 93.2 Plus
Dmoj\GI22015-PA 195 GI22015-PA 7..195 4..192 720 66.7 Plus
Dmoj\GI11109-PA 191 GI11109-PA 1..191 1..192 715 68.8 Plus
Dmoj\GI11262-PA 170 GI11262-PA 1..169 1..169 677 71 Plus
Blast to dper-all-translation-r1.3.fasta performed 2019-03-15 23:31:48
Subject Length Description Subject Range Query Range Score Percent Strand
Dper\GL24253-PA 192 GL24253-PA 1..192 1..192 974 95.8 Plus
Dper\GL16078-PA 146 GL16078-PA 1..144 1..144 749 95.8 Plus
Dper\GL13704-PA 195 GL13704-PA 7..195 4..192 716 66.1 Plus
Dper\GL18265-PA 191 GL18265-PA 1..191 1..192 712 68.2 Plus
Dper\GL20555-PA 192 GL20555-PA 7..192 5..192 562 56.4 Plus
Blast to dpse-all-translation-r3.2.fasta performed 2019-03-15 23:31:48
Subject Length Description Subject Range Query Range Score Percent Strand
Dpse\GA23612-PA 192 GA23612-PA 1..192 1..192 974 95.8 Plus
Dpse\GA15321-PA 192 GA15321-PA 1..192 1..192 959 93.2 Plus
Dpse\GA18989-PA 195 GA18989-PA 7..195 4..192 716 66.1 Plus
Dpse\GA11680-PA 191 GA11680-PA 1..191 1..192 712 68.2 Plus
Dpse\GA25121-PA 192 GA25121-PA 7..192 5..192 562 56.4 Plus
Blast to dsec-all-translation-r1.3.fasta performed 2019-03-15 23:31:49
Subject Length Description Subject Range Query Range Score Percent Strand
Dsec\GM13759-PA 192 GM13759-PA 1..192 1..192 1022 100 Plus
Dsec\GM14225-PA 192 GM14225-PA 1..192 1..192 959 93.2 Plus
Dsec\GM16346-PA 195 GM16346-PA 7..195 4..192 711 66.1 Plus
Dsec\GM11478-PA 195 GM11478-PA 7..195 4..192 711 66.1 Plus
Dsec\GM22983-PA 154 GM22983-PA 1..153 1..153 605 69.9 Plus
Blast to dsim-all-translation-r1.4.fasta performed 2019-03-15 23:31:49
Subject Length Description Subject Range Query Range Score Percent Strand
Dsim\GD13056-PA 192 GD13056-PA 1..192 1..192 1022 100 Plus
Dsim\GD13487-PA 192 GD13487-PA 1..192 1..192 959 93.2 Plus
Dsim\GD17456-PA 191 GD17456-PA 1..191 1..192 712 68.2 Plus
Dsim\GD21343-PA 184 GD21343-PA 7..168 4..165 660 70.4 Plus
Dsim\GD11158-PA 192 GD11158-PA 7..192 5..192 561 56.4 Plus
Blast to dvir-all-translation-r1.2.fasta performed 2019-03-15 23:31:50
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\GJ12360-PA 192 GJ12360-PA 1..192 1..192 971 94.8 Plus
Dvir\GJ13209-PA 192 GJ13209-PA 1..192 1..192 959 93.2 Plus
Dvir\GJ10465-PA 195 GJ10465-PA 7..195 4..192 720 66.7 Plus
Dvir\GJ15962-PA 191 GJ15962-PA 1..191 1..192 713 68.8 Plus
Dvir\GJ22286-PA 192 GJ22286-PA 7..192 5..192 562 56.9 Plus
Blast to dwil-all-translation-r1.3.fasta performed 2019-03-15 23:31:50
Subject Length Description Subject Range Query Range Score Percent Strand
Dwil\GK16805-PA 192 GK16805-PA 1..192 1..192 985 96.4 Plus
Dwil\GK20502-PA 192 GK20502-PA 1..192 1..192 955 92.7 Plus
Dwil\GK11760-PA 195 GK11760-PA 7..195 4..192 715 65.6 Plus
Dwil\GK17312-PA 191 GK17312-PA 1..191 1..192 710 68.2 Plus
Dwil\GK23137-PA 192 GK23137-PA 7..192 5..192 561 56.4 Plus
Blast to dyak-all-translation-r1.3.fasta performed 2019-03-15 23:31:51
Subject Length Description Subject Range Query Range Score Percent Strand
Dyak\GE20412-PA 192 GE20412-PA 1..192 1..192 1022 100 Plus
Dyak\GE20972-PA 192 GE20972-PA 1..192 1..192 959 93.2 Plus
Dyak\GE15867-PA 191 GE15867-PA 1..191 1..192 712 68.2 Plus
Dyak\GE23748-PA 195 GE23748-PA 7..195 4..192 711 66.1 Plus
Dyak\GE11751-PA 192 GE11751-PA 7..192 5..192 561 56.4 Plus

GM13874.hyp Sequence

Translation from 626 to 1204

> GM13874.hyp
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDAKP
INLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVNPASFENVRAKWFPE
VRHHCPSVPIILVGTKLDLRDDKQTIEKLKDKKLTPITYPQGLAMAKEIA
AVKYLECSALTQKGLKTVFDEAIRSVLCPVVRGPKRHKCALL*

GM13874.hyp Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 11:30:13
Subject Length Description Subject Range Query Range Score Percent Strand
Rac2-PB 192 CG8556-PB 1..192 1..192 1012 100 Plus
Rac2-PA 192 CG8556-PA 1..192 1..192 1012 100 Plus
Rac1-PB 192 CG2248-PB 1..192 1..192 949 93.2 Plus
Rac1-PA 192 CG2248-PA 1..192 1..192 949 93.2 Plus
Mtl-PC 195 CG5588-PC 7..195 4..192 707 66.1 Plus