Clone LD21452 Report

Search the DGRC for LD21452

Clone and Library Details

Library:LD
Tissue Source:Drosophila melanogaster embryo
Created by:Ling Hong
Date Registered:1997-12-04
Comments:Constructed using Stratagene ZAP-cDNA Synthesis kit. Oligo dT-primed and directionally cloned at EcoRI and XhoI in BlueScript SK(+/-)
Original Plate Number:214
Well:52
Vector:pOT2
Associated Gene/TranscriptTwdlW-RA
Protein status:LD21452.pep: gold
Preliminary Size:1300
Sequenced Size:1126

Associated Genes

Associations are from manual ordering of a clone or by a periodic analysis.
Gene Date Evidence
CG4060 2001-01-01 Release 2 assignment
CG4060 2002-07-13 Blastp of sequenced clone
CG4060 2003-01-01 Sim4 clustering to Release 3
TwdlW 2008-04-29 Release 5.5 accounting
TwdlW 2008-08-15 Release 5.9 accounting
TwdlW 2008-12-18 5.12 accounting

Clone Sequence Records

LD21452.complete Sequence

1126 bp (1126 high quality bases) assembled on 2002-07-13

GenBank Submission: BT001478

> LD21452.complete
AAACTCGGACAGCGAGGGAGCATCCGGCTTAAGACAGCTAACCAGCAGAG
AGACACCCACCCAAGCGGCAGCATGAAGATATTTGTGACGGTTCTGATGG
GCGCCATGTGCCTGAGCCTCAGCCAGGCTGACATCAGCTTGACCCAGGCG
GGTTACAACTACCGCATCCAACAGCAGCAGCAGCAACAGCAACCGCCCAT
TGTGGCCCACCTGTTGAACCAGCAACTGCAGCAGCAGCAGCAGCTCCAGC
CCCAGCAATCCTCGCACCCGGTGCTTCCACCTCTCCCACTGCCCTACCTC
CCGCCCGCTGGCCAGTTCCATTCTGCCCAGCCTGCAGTTCGTCCCACAGC
CGGATCGTTTCCCATGCCCGCCGGCTTCCCCGTCAATTTCCAGACCGCTC
CCATCCAGCAGCAGCGACGCGCTCGTCCGCGTGTCAGGATTCCCAAGCGT
CCCATTGTGACCAAAAACTTTTTCATCCACTCGGCGCCCGAGGAGTCTGA
GGATGAGGTCCAGGACGAGCTGAACCAGTTGGCCCAGCAGCCCCGTAACC
ACTACAATGTGCTGTTCGTCAAGACGCCGGCTCAGACCAATCGCGCGGCG
GCTCTTAACTTGGCCAAGACCCTTAAGCAGGAGAAGACCGTGGTCTATGT
GCTGGCCAAGAAGACGACCGCCTCCGATCTGCAGGATGCCATTGCCGAGG
CGCCACAGCACATCAATAAGCCGGAGGTGTTCTTCATCAAGTACCGCACT
CCAGAGGAAGCTCTGAATGCCCAGCGGCAGATTCAGTCACAGTACGATAC
TTTGGGAGGAAGTTCGACCATCACCGACGAGGGTGTGGCCCCCATCACCT
CGGTGGTGGGATCCCTGGATCCTCCAGAGGAGGAGGAGGAGGAACAGCAA
CAGCAGCAACATTCAGAGGGACAATTTGCGGAGAACGGTGCCGGAAACTC
TGGCGTCGGTCCCATCGGCAATCATTATTTGCCCGCGAACCAGTTCTAAG
TATTCTTCATTGCTCGTCACATCCAGATCTAGTTAGCTGTCATCAACACC
ACTTAGGTTTAAGTGCTTGCCAATGTTACCCAAATCCGTATTAAAATATA
ATTGTTAAAAAAAAAAAAAAAAAAAA

LD21452.complete Blast Records

Blast to MB8.fasta performed 2010-07-15 18:43:47
Subject Length Description Subject Range Query Range Score Percent Strand
TwdlW-RA 1406 TwdlW-RA 241..1356 1..1116 5580 100 Plus
Blast to d_melanogaster_OreR.fa performed 2019-03-16 21:34:03
Subject Length Description Subject Range Query Range Score Percent Strand
chr3R 27901430 chr3R 12965631..12966650 1106..87 5025 99.5 Minus
chr3R 27901430 chr3R 12966721..12966807 87..1 435 100 Minus
Blast to dmel-all-all_noncoding-r5.12.fasta performed on 2010-04-22 19:09:08 has no hits.
Blast to na_all.dmel.RELEASE6 performed 2019-03-16 21:34:00
Subject Length Description Subject Range Query Range Score Percent Strand
3R 32079331 3R 17141198..17142227 1116..87 5150 100 Minus
3R 32079331 3R 17142298..17142384 87..1 435 100 Minus
Blast to na_arms.dmel.RELEASE6 performed 2011-12-12 20:16:57
Subject Length Description Subject Range Query Range Score Percent Strand
3R 31820162 3R 16882029..16883058 1116..87 5150 100 Minus
3R 31820162 3R 16883129..16883215 87..1 435 100 Minus
Blast to na_te.dros performed 2019-03-16 21:34:01
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6754..6869 155..268 206 65.5 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6791..6892 156..258 197 67 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6704..6820 145..258 190 65 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6733..6870 155..288 184 63.3 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2391..2526 156..289 179 62 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2307..2405 159..258 173 65 Plus
roo 9092 roo DM_ROO 9092bp 1079..1161 169..257 161 68.5 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2439..2560 156..277 159 61 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2337..2430 156..250 157 64.2 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2364..2455 169..257 155 66.3 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2787..2888 156..258 152 62.1 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6808..6946 155..288 151 59.7 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6826..6920 155..244 147 66.3 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2758..2869 155..257 141 65.2 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 2590..2682 170..260 135 64.9 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6857..6893 156..193 124 84.2 Plus
gypsy11 4428 gypsy11 GYPSY11 4428bp 940..999 132..193 124 71 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 1542..1581 156..196 121 80.5 Plus
roo 9092 roo DM_ROO 9092bp 1108..1148 152..193 117 78.6 Plus
roo 9092 roo DM_ROO 9092bp 1121..1157 156..193 115 81.6 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 5636..5673 155..193 111 79.5 Plus
Dvir\TART 8500 Dvir\TART TARTVIR 8500bp 6718..6768 861..911 111 68.6 Plus

LD21452.complete Sim4 Records

Sim4 to d_melanogaster_OreR.fa performed 2019-03-16 21:34:41 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
chr3R 12965649..12966479 258..1088 99 == Minus
chr3R 12966569..12966649 88..168 100 <- Minus
chr3R 12966721..12966807 1..87 100   Minus
Sim4 to dmel-all-CDS-r5.12.fasta performed 2008-12-08 18:56:12 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
TwdlW-RA 1..927 73..999 100   Plus
Sim4 to dmel-all-CDS-r5.32.fasta performed 2011-03-16 17:17:23 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
TwdlW-RA 1..927 73..999 100   Plus
Sim4 to dmel-all-CDS-r5.52.fasta performed 2013-08-04 18:01:58 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
TwdlW-RA 1..927 73..999 100   Plus
Sim4 to dmel-all-CDS-r5.9.fasta performed on 2008-07-21 18:08:33 has no hits.
Sim4 to dmel-all-CDS-r6.02.fasta performed 2014-11-27 16:23:59 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
TwdlW-RA 1..927 73..999 100   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2008-11-10 20:41:44 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
TwdlW-RA 15..1120 1..1106 100   Plus
Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-03-16 17:17:23 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
TwdlW-RA 15..1120 1..1106 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 18:01:58 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
TwdlW-RA 21..1126 1..1106 100   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-08-04 17:07:43 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
TwdlW-RA 15..1120 1..1106 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-27 16:23:59 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
TwdlW-RA 21..1126 1..1106 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 21:34:41 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
3R 17141208..17142226 88..1106 100 <- Minus
3R 17142298..17142384 1..87 100   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 21:34:41 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
3R 17141208..17142226 88..1106 100 <- Minus
3R 17142298..17142384 1..87 100   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 21:34:41 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
3R 17141208..17142226 88..1106 100 <- Minus
3R 17142298..17142384 1..87 100   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 18:01:58 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3R 12966930..12967948 88..1106 100 <- Minus
arm_3R 12968020..12968106 1..87 100   Minus
Sim4 to na_arms.dmel.RELEASE6 performed 2011-12-09 14:40:32 Download gff for LD21452.complete
Subject Subject Range Query Range Percent Splice Strand
3R 16882039..16883057 88..1106 100 <- Minus
3R 16883129..16883215 1..87 100   Minus

LD21452.hyp Sequence

Translation from 0 to 998

> LD21452.hyp
KLGQRGSIRLKTANQQRDTHPSGSMKIFVTVLMGAMCLSLSQADISLTQA
GYNYRIQQQQQQQQPPIVAHLLNQQLQQQQQLQPQQSSHPVLPPLPLPYL
PPAGQFHSAQPAVRPTAGSFPMPAGFPVNFQTAPIQQQRRARPRVRIPKR
PIVTKNFFIHSAPEESEDEVQDELNQLAQQPRNHYNVLFVKTPAQTNRAA
ALNLAKTLKQEKTVVYVLAKKTTASDLQDAIAEAPQHINKPEVFFIKYRT
PEEALNAQRQIQSQYDTLGGSSTITDEGVAPITSVVGSLDPPEEEEEEQQ
QQQHSEGQFAENGAGNSGVGPIGNHYLPANQF*

LD21452.hyp Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 16:14:39
Subject Length Description Subject Range Query Range Score Percent Strand
TwdlW-PA 308 CG4060-PA 1..308 25..332 1590 100 Plus
TwdlV-PA 251 CG14640-PA 51..250 113..330 376 42.5 Plus
TwdlG-PC 278 CG14643-PC 19..275 51..330 371 35.5 Plus
TwdlG-PB 278 CG14643-PB 19..275 51..330 371 35.5 Plus
TwdlG-PA 278 CG14643-PA 19..275 51..330 371 35.5 Plus

LD21452.pep Sequence

Translation from 72 to 998

> LD21452.pep
MKIFVTVLMGAMCLSLSQADISLTQAGYNYRIQQQQQQQQPPIVAHLLNQ
QLQQQQQLQPQQSSHPVLPPLPLPYLPPAGQFHSAQPAVRPTAGSFPMPA
GFPVNFQTAPIQQQRRARPRVRIPKRPIVTKNFFIHSAPEESEDEVQDEL
NQLAQQPRNHYNVLFVKTPAQTNRAAALNLAKTLKQEKTVVYVLAKKTTA
SDLQDAIAEAPQHINKPEVFFIKYRTPEEALNAQRQIQSQYDTLGGSSTI
TDEGVAPITSVVGSLDPPEEEEEEQQQQQHSEGQFAENGAGNSGVGPIGN
HYLPANQF*

LD21452.pep Blast Records

Blast to dana-all-translation-r1.3.fasta performed 2019-03-16 04:39:39
Subject Length Description Subject Range Query Range Score Percent Strand
Dana\GF17860-PA 309 GF17860-PA 1..309 1..308 1114 84.5 Plus
Dana\GF16822-PA 238 GF16822-PA 71..212 128..268 342 49.3 Plus
Dana\GF19934-PA 278 GF19934-PA 67..209 126..263 332 52.1 Plus
Dana\GF18197-PA 263 GF18197-PA 90..262 128..306 317 47.2 Plus
Dana\GF22658-PA 345 GF22658-PA 138..273 129..265 291 43.8 Plus
Blast to dere-all-translation-r1.3.fasta performed 2019-03-16 04:39:40
Subject Length Description Subject Range Query Range Score Percent Strand
Dere\GG16788-PA 301 GG16788-PA 1..301 1..308 1236 87.8 Plus
Dere\GG11741-PA 269 GG11741-PA 104..266 129..306 352 44.1 Plus
Dere\GG12219-PA 347 GG12219-PA 137..284 125..266 326 50.3 Plus
Dere\GG12230-PA 252 GG12230-PA 77..251 128..306 318 46.7 Plus
Dere\GG19072-PA 322 GG19072-PA 123..258 129..265 296 45.3 Plus
Blast to dgri-all-translation-r1.3.fasta performed 2019-03-16 04:39:42
Subject Length Description Subject Range Query Range Score Percent Strand
Dgri\GH14248-PA 305 GH14248-PA 95..305 96..308 925 86.4 Plus
Dgri\GH21845-PA 288 GH21845-PA 107..246 127..266 359 48.6 Plus
Dgri\GH19057-PA 287 GH19057-PA 84..220 128..265 355 52.2 Plus
Dgri\GH19046-PA 379 GH19046-PA 98..274 126..293 310 42.6 Plus
Dgri\GH11916-PA 242 GH11916-PA 73..211 127..266 294 45 Plus
Blast to dmel-all-translation-r6.23.fasta performed 2019-03-25 10:10:14
Subject Length Description Subject Range Query Range Score Percent Strand
TwdlW-PA 308 CG4060-PA 1..308 1..308 1590 100 Plus
TwdlV-PA 251 CG14640-PA 51..250 89..306 376 42.5 Plus
TwdlG-PC 278 CG14643-PC 19..275 27..306 371 35.5 Plus
TwdlG-PB 278 CG14643-PB 19..275 27..306 371 35.5 Plus
TwdlG-PA 278 CG14643-PA 19..275 27..306 371 35.5 Plus
TwdlF-PA 354 CG14639-PA 82..281 66..266 341 41.5 Plus
Twdlalpha-PA 388 CG32574-PA 153..350 109..302 313 37.7 Plus
TwdlY-PA 247 CG32570-PA 76..214 127..266 301 43.6 Plus
TwdlX-PA 346 CG32571-PA 147..282 129..265 300 44.5 Plus
TwdlZ-PB 210 CG32569-PB 19..206 113..298 277 34.6 Plus
TwdlQ-PA 245 CG14250-PA 76..228 130..278 273 40.9 Plus
Tb-PA 283 CG5480-PA 90..239 131..279 263 38.8 Plus
TwdlD-PA 256 CG14243-PA 33..215 73..264 262 36.4 Plus
TwdlB-PA 286 CG6478-PA 126..253 131..257 257 48.8 Plus
TwdlL-PB 279 CG6447-PB 120..247 131..257 256 48.8 Plus
TwdlL-PA 285 CG6447-PA 126..253 131..257 256 48.8 Plus
TwdlJ-PB 274 CG5471-PB 39..267 78..306 247 33.9 Plus
TwdlM-PA 288 CG5468-PA 130..258 131..258 245 45.4 Plus
TwdlN-PA 309 CG5476-PA 147..273 131..256 245 47.7 Plus
TwdlH-PA 241 CG31080-PA 81..209 131..258 236 43.8 Plus
TwdlK-PA 247 CG6460-PA 36..225 78..269 232 35.1 Plus
TwdlP-PA 220 CG14240-PA 34..208 93..280 230 34.7 Plus
TwdlO-PA 229 CG6452-PA 66..186 140..256 225 45.5 Plus
TwdlR-PA 325 CG31081-PA 70..228 131..307 215 35.8 Plus
TwdlC-PA 360 CG14254-PA 28..308 28..308 198 27.4 Plus
TwdlT-PA 286 CG5812-PA 130..253 128..254 170 34.9 Plus
Blast to dmoj-all-translation-r1.3.fasta performed 2019-03-16 04:39:43
Subject Length Description Subject Range Query Range Score Percent Strand
Dmoj\GI23141-PA 328 GI23141-PA 1..328 1..308 1002 69.6 Plus
Dmoj\GI10848-PA 268 GI10848-PA 84..265 127..304 369 45.9 Plus
Dmoj\GI21932-PA 273 GI21932-PA 93..270 127..306 360 41.9 Plus
Dmoj\GI10847-PA 372 GI10847-PA 146..289 125..264 303 47.9 Plus
Dmoj\GI14654-PA 562 GI14654-PA 393..531 127..266 301 45.4 Plus
Blast to dper-all-translation-r1.3.fasta performed 2019-03-16 04:39:44
Subject Length Description Subject Range Query Range Score Percent Strand
Dper\GL27212-PA 312 GL27212-PA 1..312 1..308 1008 84.6 Plus
Dper\GL12334-PA 296 GL12334-PA 130..293 128..306 338 44.4 Plus
Dper\GL12309-PA 437 GL12309-PA 264..397 128..265 302 53.6 Plus
Dper\GL24435-PA 251 GL24435-PA 83..212 130..258 258 45.1 Plus
Dper\GL26861-PA 223 GL26861-PA 34..173 127..265 257 41.8 Plus
Blast to dpse-all-translation-r3.2.fasta performed 2019-03-16 04:39:45
Subject Length Description Subject Range Query Range Score Percent Strand
Dpse\GA17925-PA 314 GA17925-PA 1..314 1..308 991 82.8 Plus
Dpse\GA13142-PA 296 GA13142-PA 130..293 128..306 337 44.4 Plus
Dpse\GA13137-PA 353 GA13137-PA 148..291 125..263 333 50.3 Plus
Dpse\GA13139-PA 256 GA13139-PA 83..216 128..265 303 53.6 Plus
Dpse\GA16997-PA 254 GA16997-PA 78..216 127..266 292 44.3 Plus
Blast to dsec-all-translation-r1.3.fasta performed 2019-03-16 04:39:46
Subject Length Description Subject Range Query Range Score Percent Strand
Dsec\GM15381-PA 306 GM15381-PA 1..306 1..308 1548 97.4 Plus
Dsec\GM10719-PA 281 GM10719-PA 116..278 129..306 350 44.1 Plus
Dsec\GM10738-PA 357 GM10738-PA 137..284 125..266 324 50.3 Plus
Dsec\GM10739-PA 254 GM10739-PA 81..253 128..306 321 47.8 Plus
Dsec\GM13463-PA 247 GM13463-PA 76..214 127..266 292 44.3 Plus
Blast to dsim-all-translation-r1.4.fasta performed 2019-03-16 04:39:47
Subject Length Description Subject Range Query Range Score Percent Strand
Dsim\GD20247-PA 303 GD20247-PA 1..303 1..308 1539 97.1 Plus
Dsim\GD19694-PA 278 GD19694-PA 113..275 129..306 347 43.6 Plus
Dsim\GD19709-PA 357 GD19709-PA 137..284 125..266 325 50.3 Plus
Dsim\GD19711-PA 255 GD19711-PA 82..254 128..306 321 47.8 Plus
Dsim\GD17302-PA 346 GD17302-PA 147..282 129..265 291 44.5 Plus
Blast to dvir-all-translation-r1.2.fasta performed 2019-03-16 04:39:48
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\GJ10443-PA 300 GJ10443-PA 1..300 1..308 1014 80.1 Plus
Dvir\GJ14455-PA 269 GJ14455-PA 89..223 127..262 391 53.7 Plus
Dvir\GJ14291-PA 281 GJ14291-PA 111..278 127..306 350 43.1 Plus
Dvir\GJ14454-PA 344 GJ14454-PA 118..261 125..264 301 47.9 Plus
Dvir\GJ19306-PA 246 GJ19306-PA 77..215 127..266 295 44.3 Plus
Blast to dwil-all-translation-r1.3.fasta performed 2019-03-16 04:39:50
Subject Length Description Subject Range Query Range Score Percent Strand
Dwil\GK10882-PA 315 GK10882-PA 1..315 1..308 1006 78.5 Plus
Dwil\GK22650-PA 292 GK22650-PA 120..289 128..306 341 43.6 Plus
Dwil\GK22519-PA 265 GK22519-PA 91..264 128..306 326 46.7 Plus
Dwil\GK22518-PA 337 GK22518-PA 136..278 126..263 312 47.2 Plus
Dwil\GK25415-PA 249 GK25415-PA 82..220 127..266 296 45 Plus
Blast to dyak-all-translation-r1.3.fasta performed 2019-03-16 04:39:50
Subject Length Description Subject Range Query Range Score Percent Strand
Dyak\GE24178-PA 269 GE24178-PA 71..269 115..308 907 94.5 Plus
Dyak\GE25368-PA 265 GE25368-PA 82..238 109..266 342 47.2 Plus
Dyak\GE25399-PA 334 GE25399-PA 124..271 125..266 329 51 Plus
Dyak\GE25400-PA 250 GE25400-PA 77..249 128..306 312 47.8 Plus
Dyak\GE17618-PA 247 GE17618-PA 76..214 127..266 293 44.3 Plus