Clone LD28127 Report

Search the DGRC for LD28127

Clone and Library Details

Library:LD
Tissue Source:Drosophila melanogaster embryo
Created by:Ling Hong
Date Registered:1997-12-04
Comments:Constructed using Stratagene ZAP-cDNA Synthesis kit. Oligo dT-primed and directionally cloned at EcoRI and XhoI in BlueScript SK(+/-)
Original Plate Number:281
Well:27
Vector:pOT2
Associated Gene/TranscriptCG17746-RA
Protein status:LD28127.pep: gold
Preliminary Size:1809
Sequenced Size:1601

Associated Genes

Associations are from manual ordering of a clone or by a periodic analysis.
Gene Date Evidence
CG17746 2001-01-01 Release 2 assignment
CG17746 2001-12-12 Blastp of sequenced clone
CG17746 2003-01-01 Sim4 clustering to Release 3
CG17746 2008-04-29 Release 5.5 accounting
CG17746 2008-08-15 Release 5.9 accounting
CG17746 2008-12-18 5.12 accounting

Clone Sequence Records

LD28127.complete Sequence

1601 bp (1601 high quality bases) assembled on 2001-12-12

GenBank Submission: AY070552

> LD28127.complete
TTTTTCAGACACGTTCACACTTCAAAACACAAATTCAATAAAAAAATAAA
CACGCGCAGTTTTTAATAATTTTTTACCAAACAGTTTTTCTTTGATGCGT
ACTCGCTTTGCAGGGTGGAGCAAAAACGGTGAACGCCAGAAAAGCGACGT
AAATCGATAGCACTAACCCCGCCCGCAAAGAAGTCAGCCCACACAACACA
CTCCACACCGCACACCATACAAGCCGCAATGGGTCAGACTCTATCGGAAC
CGGTGACCGCCAAGGAGTCAGCGTACTGCCAGAACGCCGCCTACCGCGTG
GGCAGCTCCTGCATGCAGGGGTGGCGCATCAACATGGAGGACTCGCACAC
CCACATCCTCTCGCTGCCGGACGATCCGGGAGCGGCATTCTTCGCCGTCT
ACGACGGGCACGGAGGCGCCACGGTGGCCCAGTACGCCGGCAAGCACCTG
CACAAGTACGTTCTCAAGCGGCCGGAGTACAACGACAACATCGAACAGGC
GCTGCAGCAGGGCTTTCTGGACATCGACTACGTTATGCTACGCAACAAGA
CATGTGGTGACCAGATGGCCGGCTCGACTGCCGTAGTTGTTTTGGTAAAG
GACAACAAGCTTTATTGCGCCAACGCAGGCGACTCGCGCGCCATTGCCTG
CGTGAACGGACAACTGGAGGTCCTGTCGCTGGACCACAAGCCCAATAACG
AGGCGGAGTCCAAGCGGATCATCCAAGGCGGCGGCTGGGTTGAGTTCAAT
CGCGTCAACGGCAATCTTGCGCTTTCCCGCGCCCTCGGTGACTATGTATT
CAAGCATGAGAACAAAAAACCGGAAGATCAAATCGTCACAGCCTTCCCGG
ACGTGGAGACGCGGAAAATCATGGACGATTGGGAATTCATCGTGCTGGCC
TGCGATGGCATTTGGGATGTAATGAGCAACGCGGAAGTGCTCGAGTTCTG
TCGCACGCGCATCGGCATGGGCATGTTTCCGGAGGAGATCTGCGAAGAGC
TGATGAATCACTGCCTAGCACCCGACTGCCAAATGGGAGGCCTCGGCGGC
GACAATATGACCGTGGTGCTGGTGTGCCTGCTGCACGGCCGTCCCTACAG
CGATCTGATAGCCCGCTGCCGAAATGGCAGCCAGGCGACTAACAATGATC
AAGAGGAAGCCGGCGACGCGACGAAGGATGAAGTCGCAAAGGACGAAGCG
GAGGCGGAAACCGATACCGAAACGGAAACGCAAACCAAACCCTGCCAGAA
GCAATCCAATTCCGGTCCCATGGACGACGAGGTGAAGATGGCGTCGCATC
TAAACAAGATGGCAGCGATCGAAAGCCACTATATATACGATTAATTAGGG
TTACAGAAATAGCGCTTAGGTCAACTCTTTATACTTAACAGCGCAAGTGT
TCTTGGTTAAGTTTTATATACACACAGTACGCCACACAGTCCTTACCAAA
ATATTTGCTCCGATTATTAATAATTGTTTTAACTGTAACCCCACGATGCG
TGTATATGCCTTGTCTAACTATTTAATGTATGATTTACTATGCTTTGTGA
TGCCCGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
A

LD28127.complete Blast Records

Blast to MB8.fasta performed 2010-07-15 19:49:55
Subject Length Description Subject Range Query Range Score Percent Strand
CG17746.c 1972 CG17746.c 187..1749 1..1563 7815 100 Plus
CG17746-RA 1805 CG17746-RA 20..1582 1..1563 7815 100 Plus
CG17746.b 2516 CG17746.b 560..2122 1..1563 7815 100 Plus
Blast to d_melanogaster_OreR.fa performed 2019-03-16 11:34:24
Subject Length Description Subject Range Query Range Score Percent Strand
chr3L 24539361 chr3L 3236355..3237074 1559..840 3600 100 Minus
chr3L 24539361 chr3L 3238019..3238393 511..137 1875 100 Minus
chr3L 24539361 chr3L 3237150..3237484 841..507 1675 100 Minus
chr3L 24539361 chr3L 3238830..3238968 139..1 695 100 Minus
chr3L 24539361 chr3L 581417..582282 227..1086 490 71.2 Plus
Blast to dmel-all-all_noncoding-r5.12.fasta performed on 2010-04-22 19:20:41 has no hits.
Blast to na_all.dmel.RELEASE6 performed 2019-03-16 11:34:22
Subject Length Description Subject Range Query Range Score Percent Strand
3L 28110227 3L 3236943..3237666 1563..840 3620 100 Minus
3L 28110227 3L 3238611..3238985 511..137 1875 100 Minus
3L 28110227 3L 3237742..3238076 841..507 1675 100 Minus
3L 28110227 3L 3239422..3239560 139..1 695 100 Minus
3L 28110227 3L 581561..582426 227..1086 475 71.1 Plus
Blast to na_arms.dmel.RELEASE6 performed 2011-12-12 21:15:56
Subject Length Description Subject Range Query Range Score Percent Strand
3L 28103327 3L 3236943..3237666 1563..840 3620 100 Minus
3L 28103327 3L 3238611..3238985 511..137 1875 100 Minus
3L 28103327 3L 3237742..3238076 841..507 1675 100 Minus
3L 28103327 3L 3239422..3239560 139..1 695 100 Minus
3L 28103327 3L 581625..581813 291..479 360 79.3 Plus
3L 28103327 3L 582009..582130 672..793 265 81.1 Plus
3L 28103327 3L 582385..582426 1045..1086 150 90.4 Plus
Blast to na_te.dros performed on 2019-03-16 11:34:23 has no hits.

LD28127.complete Sim4 Records

Sim4 to d_melanogaster_OreR.fa performed 2019-03-16 11:35:30 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
chr3L 3238020..3238390 140..510 100 <- Minus
chr3L 3238830..3238968 1..139 100   Minus
chr3L 3236355..3237072 842..1559 100 <- Minus
chr3L 3237150..3237480 511..841 100 <- Minus
Sim4 to dmel-all-CDS-r5.12.fasta performed 2008-12-08 19:07:50 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
CG17746-RB 1..1116 229..1344 100   Plus
Sim4 to dmel-all-CDS-r5.32.fasta performed 2011-03-16 19:18:54 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
CG17746-RB 1..1116 229..1344 100   Plus
Sim4 to dmel-all-CDS-r5.52.fasta performed 2013-08-04 10:30:33 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
CG17746-RA 1..1116 229..1344 100   Plus
Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-21 19:45:01 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
CG17746-RB 1..1116 229..1344 100   Plus
Sim4 to dmel-all-CDS-r6.02.fasta performed 2014-11-27 10:20:53 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
CG17746-RA 1..1116 229..1344 100   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2008-11-10 22:52:05 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
CG17746-RA 1..1519 41..1559 100   Plus
Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-03-16 19:18:54 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
CG17746-RA 1..1519 41..1559 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 10:30:33 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
CG17746-RA 19..1577 1..1559 100   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-21 19:45:02 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
CG17746-RA 1..1519 41..1559 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-27 10:20:53 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
CG17746-RA 19..1577 1..1559 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 11:35:30 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
3L 3236947..3237664 842..1559 100 <- Minus
3L 3237742..3238072 511..841 100 <- Minus
3L 3238612..3238982 140..510 100 <- Minus
3L 3239422..3239560 1..139 100   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 11:35:30 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
3L 3236947..3237664 842..1559 100 <- Minus
3L 3237742..3238072 511..841 100 <- Minus
3L 3238612..3238982 140..510 100 <- Minus
3L 3239422..3239560 1..139 100   Minus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 11:35:30 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
3L 3236947..3237664 842..1559 100 <- Minus
3L 3237742..3238072 511..841 100 <- Minus
3L 3238612..3238982 140..510 100 <- Minus
3L 3239422..3239560 1..139 100   Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 10:30:33 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3L 3236947..3237664 842..1559 100 <- Minus
arm_3L 3237742..3238072 511..841 100 <- Minus
arm_3L 3238612..3238982 140..510 100 <- Minus
arm_3L 3239422..3239560 1..139 100   Minus
Sim4 to na_arms.dmel.RELEASE6 performed 2011-12-09 16:21:21 Download gff for LD28127.complete
Subject Subject Range Query Range Percent Splice Strand
3L 3236947..3237664 842..1559 100 <- Minus
3L 3237742..3238072 511..841 100 <- Minus
3L 3238612..3238982 140..510 100 <- Minus
3L 3239422..3239560 1..139 100   Minus

LD28127.pep Sequence

Translation from 228 to 1343

> LD28127.pep
MGQTLSEPVTAKESAYCQNAAYRVGSSCMQGWRINMEDSHTHILSLPDDP
GAAFFAVYDGHGGATVAQYAGKHLHKYVLKRPEYNDNIEQALQQGFLDID
YVMLRNKTCGDQMAGSTAVVVLVKDNKLYCANAGDSRAIACVNGQLEVLS
LDHKPNNEAESKRIIQGGGWVEFNRVNGNLALSRALGDYVFKHENKKPED
QIVTAFPDVETRKIMDDWEFIVLACDGIWDVMSNAEVLEFCRTRIGMGMF
PEEICEELMNHCLAPDCQMGGLGGDNMTVVLVCLLHGRPYSDLIARCRNG
SQATNNDQEEAGDATKDEVAKDEAEAETDTETETQTKPCQKQSNSGPMDD
EVKMASHLNKMAAIESHYIYD*

LD28127.pep Blast Records

Blast to dana-all-translation-r1.3.fasta performed 2019-03-16 09:12:57
Subject Length Description Subject Range Query Range Score Percent Strand
Dana\GF24378-PA 374 GF24378-PA 1..325 1..329 1545 86.1 Plus
Dana\GF24758-PA 349 GF24758-PA 1..312 1..313 1165 66 Plus
Dana\GF20730-PA 366 GF20730-PA 1..311 1..323 463 35 Plus
Dana\GF23353-PA 371 GF23353-PA 1..313 1..319 451 35.5 Plus
Dana\GF18976-PA 707 GF18976-PA 400..571 114..282 393 47.7 Plus
Dana\GF18976-PA 707 GF18976-PA 1..103 1..104 208 43.8 Plus
Blast to dere-all-translation-r1.3.fasta performed 2019-03-16 09:12:58
Subject Length Description Subject Range Query Range Score Percent Strand
Dere\GG14269-PA 370 GG14269-PA 1..370 1..371 1814 94.3 Plus
Dere\GG14729-PA 353 GG14729-PA 1..320 1..321 1159 66.9 Plus
Dere\GG12191-PA 367 GG12191-PA 1..273 1..278 464 38.1 Plus
Dere\GG11671-PA 374 GG11671-PA 1..284 1..284 463 37.6 Plus
Dere\GG10863-PA 664 GG10863-PA 394..564 114..282 415 49.7 Plus
Dere\GG10863-PA 664 GG10863-PA 1..109 1..110 216 44.1 Plus
Blast to dgri-all-translation-r1.3.fasta performed 2019-03-16 09:12:58
Subject Length Description Subject Range Query Range Score Percent Strand
Dgri\GH15705-PA 335 GH15705-PA 1..330 1..338 1365 75.8 Plus
Dgri\GH16235-PA 323 GH16235-PA 1..306 1..304 1079 64.1 Plus
Dgri\GH18902-PA 371 GH18902-PA 1..320 1..329 448 34.3 Plus
Dgri\GH20157-PA 774 GH20157-PA 481..652 114..282 409 50 Plus
Dgri\GH23728-PA 302 GH23728-PA 9..180 114..282 405 50 Plus
Dgri\GH20157-PA 774 GH20157-PA 1..103 1..104 215 43.8 Plus
Blast to dmel-all-translation-r6.23.fasta performed 2019-03-25 10:54:30
Subject Length Description Subject Range Query Range Score Percent Strand
CG17746-PB 371 CG17746-PB 1..371 1..371 1982 100 Plus
CG17746-PA 371 CG17746-PA 1..371 1..371 1982 100 Plus
Ppm1-PA 352 CG12169-PA 1..299 1..297 1110 68.9 Plus
CG6036-PA 371 CG6036-PA 5..293 1..295 505 39.6 Plus
alph-PF 332 CG1906-PF 1..282 1..282 457 38.2 Plus
alph-PC 368 CG1906-PC 1..282 1..282 457 38.2 Plus
alph-PD 368 CG1906-PD 1..282 1..282 457 38.2 Plus
alph-PA 368 CG1906-PA 1..282 1..282 457 38.2 Plus
alph-PB 371 CG1906-PB 1..282 1..282 457 38.2 Plus
alph-PE 374 CG1906-PE 1..282 1..282 457 38.2 Plus
CG10417-PB 662 CG10417-PB 392..653 114..353 398 38.2 Plus
CG10417-PA 662 CG10417-PA 392..653 114..353 398 38.2 Plus
CG7115-PC 469 CG7115-PC 240..392 87..240 242 38.4 Plus
CG7115-PD 471 CG7115-PD 242..394 87..240 242 38.4 Plus
CG7115-PE 524 CG7115-PE 295..447 87..240 242 38.4 Plus
CG7115-PB 524 CG7115-PB 295..447 87..240 242 38.4 Plus
CG7115-PA 524 CG7115-PA 295..447 87..240 242 38.4 Plus
Pp2C1-PB 1427 CG2984-PB 257..537 23..260 221 27 Plus
Pp2C1-PA 1427 CG2984-PA 257..537 23..260 221 27 Plus
CG10376-PA 428 CG10376-PA 190..419 49..284 209 29.7 Plus
CG10417-PB 662 CG10417-PB 1..103 1..104 201 45.7 Plus
CG10417-PA 662 CG10417-PA 1..103 1..104 201 45.7 Plus
Blast to dmoj-all-translation-r1.3.fasta performed 2019-03-16 09:12:59
Subject Length Description Subject Range Query Range Score Percent Strand
Dmoj\GI12760-PA 328 GI12760-PA 1..322 1..326 1434 80.7 Plus
Dmoj\GI16663-PA 329 GI16663-PA 1..298 1..296 1060 67.1 Plus
Dmoj\GI23119-PA 371 GI23119-PA 1..284 1..284 458 37.3 Plus
Dmoj\GI20667-PA 747 GI20667-PA 430..618 103..282 414 47.6 Plus
Dmoj\GI21350-PA 520 GI21350-PA 292..479 87..284 265 33.6 Plus
Dmoj\GI20667-PA 747 GI20667-PA 1..103 1..104 222 46.7 Plus
Blast to dper-all-translation-r1.3.fasta performed 2019-03-16 09:12:59
Subject Length Description Subject Range Query Range Score Percent Strand
Dper\GL21276-PA 375 GL21276-PA 1..321 1..322 1548 87.3 Plus
Dper\GL22499-PA 319 GL22499-PA 1..305 1..303 1007 60 Plus
Dper\GL22500-PA 319 GL22500-PA 1..299 1..297 974 59.9 Plus
Dper\GL26287-PA 366 GL26287-PA 1..283 1..289 486 39.2 Plus
Dper\GL13894-PA 370 GL13894-PA 1..283 1..284 460 37.6 Plus
Blast to dpse-all-translation-r3.2.fasta performed 2019-03-16 09:13:00
Subject Length Description Subject Range Query Range Score Percent Strand
Dpse\GA14642-PA 375 GA14642-PA 1..321 1..322 1548 87.3 Plus
Dpse\GA24456-PA 319 GA24456-PA 1..305 1..303 1005 60 Plus
Dpse\GA24460-PA 319 GA24460-PA 1..299 1..297 981 60.2 Plus
Dpse\GA28805-PA 366 GA28805-PA 1..283 1..289 485 39.2 Plus
Dpse\GA15122-PA 370 GA15122-PA 1..283 1..284 460 37.6 Plus
Blast to dsec-all-translation-r1.3.fasta performed 2019-03-16 09:13:01
Subject Length Description Subject Range Query Range Score Percent Strand
Dsec\GM14062-PA 370 GM14062-PA 1..370 1..371 1946 97 Plus
Dsec\GM14345-PA 353 GM14345-PA 1..320 1..321 1144 65.9 Plus
Dsec\GM12795-PA 374 GM12795-PA 1..284 1..284 461 37.6 Plus
Dsec\GM13564-PA 319 GM13564-PA 1..284 1..284 460 37.6 Plus
Dsec\GM11092-PA 662 GM11092-PA 392..562 114..282 406 49.1 Plus
Dsec\GM11092-PA 662 GM11092-PA 1..103 1..104 213 45.7 Plus
Blast to dsim-all-translation-r1.4.fasta performed 2019-03-16 09:13:01
Subject Length Description Subject Range Query Range Score Percent Strand
Dsim\GD13337-PA 370 GD13337-PA 1..370 1..371 1944 97 Plus
Dsim\GD17616-PA 353 GD17616-PA 1..320 1..321 1119 64.7 Plus
Dsim\GD21442-PA 374 GD21442-PA 1..284 1..284 461 37.6 Plus
Dsim\GD18139-PA 305 GD18139-PA 16..211 59..278 358 38.5 Plus
Dsim\GD23491-PA 524 GD23491-PA 295..482 87..284 254 33.2 Plus
Blast to dvir-all-translation-r1.2.fasta performed 2019-03-16 09:13:02
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\GJ15526-PA 329 GJ15526-PA 1..329 1..340 1453 79.2 Plus
Dvir\GJ12915-PA 335 GJ12915-PA 1..313 1..313 1108 65.4 Plus
Dvir\GJ10608-PA 371 GJ10608-PA 1..284 1..284 454 37.1 Plus
Dvir\GJ20416-PA 729 GJ20416-PA 450..621 114..282 409 50 Plus
Dvir\GJ11141-PA 520 GJ11141-PA 294..481 87..284 266 33.6 Plus
Dvir\GJ20416-PA 729 GJ20416-PA 1..103 1..104 221 45.7 Plus
Blast to dwil-all-translation-r1.3.fasta performed 2019-03-16 09:13:03
Subject Length Description Subject Range Query Range Score Percent Strand
Dwil\GK10512-PA 391 GK10512-PA 1..338 1..336 1496 80.8 Plus
Dwil\GK16640-PA 378 GK16640-PA 11..320 1..308 1168 67.7 Plus
Dwil\GK22356-PA 170 GK22356-PA 2..138 162..298 510 64.2 Plus
Dwil\GK11903-PA 371 GK11903-PA 1..284 1..284 446 37.4 Plus
Dwil\GK10630-PA 721 GK10630-PA 441..615 114..285 388 46.9 Plus
Dwil\GK10630-PA 721 GK10630-PA 1..103 1..104 199 42.9 Plus
Blast to dyak-all-translation-r1.3.fasta performed 2019-03-16 09:13:03
Subject Length Description Subject Range Query Range Score Percent Strand
Dyak\GE20697-PA 370 GE20697-PA 1..370 1..371 1835 95.1 Plus
Dyak\GE21091-PA 358 GE21091-PA 1..320 1..321 1176 67.8 Plus
Dyak\GE10634-PA 367 GE10634-PA 1..273 1..278 482 40.4 Plus
Dyak\GE23859-PA 374 GE23859-PA 1..284 1..284 463 37.6 Plus
Dyak\GE11243-PA 634 GE11243-PA 364..534 114..282 411 49.7 Plus
Dyak\GE11243-PA 634 GE11243-PA 1..103 1..104 213 45.7 Plus

LD28127.hyp Sequence

Translation from 228 to 1343

> LD28127.hyp
MGQTLSEPVTAKESAYCQNAAYRVGSSCMQGWRINMEDSHTHILSLPDDP
GAAFFAVYDGHGGATVAQYAGKHLHKYVLKRPEYNDNIEQALQQGFLDID
YVMLRNKTCGDQMAGSTAVVVLVKDNKLYCANAGDSRAIACVNGQLEVLS
LDHKPNNEAESKRIIQGGGWVEFNRVNGNLALSRALGDYVFKHENKKPED
QIVTAFPDVETRKIMDDWEFIVLACDGIWDVMSNAEVLEFCRTRIGMGMF
PEEICEELMNHCLAPDCQMGGLGGDNMTVVLVCLLHGRPYSDLIARCRNG
SQATNNDQEEAGDATKDEVAKDEAEAETDTETETQTKPCQKQSNSGPMDD
EVKMASHLNKMAAIESHYIYD*

LD28127.hyp Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 16:49:20
Subject Length Description Subject Range Query Range Score Percent Strand
CG17746-PB 371 CG17746-PB 1..371 1..371 1982 100 Plus
CG17746-PA 371 CG17746-PA 1..371 1..371 1982 100 Plus
Ppm1-PA 352 CG12169-PA 1..299 1..297 1110 68.9 Plus
CG6036-PA 371 CG6036-PA 5..293 1..295 505 39.6 Plus
alph-PF 332 CG1906-PF 1..282 1..282 457 38.2 Plus