Clone LD44221 Report

Search the DGRC for LD44221

Clone and Library Details

Library:LD
Tissue Source:Drosophila melanogaster embryo
Created by:Ling Hong
Date Registered:1997-12-04
Comments:Constructed using Stratagene ZAP-cDNA Synthesis kit. Oligo dT-primed and directionally cloned at EcoRI and XhoI in BlueScript SK(+/-)
Original Plate Number:442
Well:21
Vector:pOT2
Associated Gene/TranscriptCG31549-RA
Protein status:LD44221.pep: gold
Preliminary Size:3208
Sequenced Size:978

Associated Genes

Associations are from manual ordering of a clone or by a periodic analysis.
Gene Date Evidence
CG11005 2001-01-01 Release 2 assignment
CG31549 2002-03-20 Blastp of sequenced clone
CG31549 2003-01-01 Sim4 clustering to Release 3
CG31549 2008-04-29 Release 5.5 accounting
CG31549 2008-08-15 Release 5.9 accounting
CG31549 2008-12-18 5.12 accounting

Clone Sequence Records

LD44221.complete Sequence

978 bp (978 high quality bases) assembled on 2002-03-20

GenBank Submission: AY094836

> LD44221.complete
CTTAGCGTAAAAGAAACACCCCACGATGTCATCCTTCAAGGACAAAGTGA
TTATTGTTACCGGCGCCAGCTCCGGCATCGGAGCAAGTGCCGCCGTCCAT
TTGGCGAAACTCGGAGGGCTCCTGGTCATCGTGGGTCGCAACGAGGAGAA
GCTGAAGGAGACGGCCGACAACATTGTGGCGGCCGGAGGAGCAACGCCAT
TGGAACTGCAGGCGGACATGACCAAGGAGGCGGAGGTGCAGCAGATTGTG
GGCGCCACTCTGGCCAAGCACGGTCGCATCGATGTGCTCGTTAACAATGC
GGGCATCCTGGAGACAGGCTCCATCGAAGCCACCAGCCTGGAGCAATTCG
ATCGTCTGATGAACACCAATGTGCGTTCCCTGTACCAACTAACCATGCTG
GCCACTCCAGAGCTAGTCAAGACCAAGGGCAATATCGTAAACGTATCCAG
TGTCTGTGGACTGCGTGCCTTTCCTGGTGTCCTGGCCTACAATGTGTCTA
AGGCAGCGGTGGACCAGTTCACGGCCTGCATAGCCCTGGAACTGGCCCCC
AAAGGAGTCCGCGTAAACGCCGTGAATCCCGGCGTGATTGTGACTGACAT
TCACAAGCGTGGCGGCATGGACGAGGAGACCTATGCCAAGTTCCTCGAAC
ACTGCAAGATAACCCACGCCTTGGGCCGTCCTGGCGATGTCAAGGAGGTG
GCTGCCGCCATTGCCTTCCTGGCCAGCGATCAGGCCAGCTTCACCACGGG
AATCAGTCTGCCCGTCGATGGAGGTCGTCACGCCATGTGCCCGCGATAGG
ATGCGGATGAGCAAATGGTGGCATTTATCGAATTTAGCGTCATTTTCAGT
AGTTAATTTAAAGAACACGACTAAAATACAACACAATACATTAAAAGTGC
GCAGTTAAGATTTCTTGGATTTTTCCTCTCATAATAAACTAATATATTGA
TAAAAAAAAAAAAAAAAAAAAAAAAAAA

LD44221.complete Blast Records

Blast to MB8.fasta performed 2010-07-15 19:20:15
Subject Length Description Subject Range Query Range Score Percent Strand
CG31549-RA 1130 CG31549-RA 180..1130 1..951 4755 100 Plus
CG31548-RA 946 CG31548-RA 623..804 611..792 520 85.7 Plus
CG12171-RA 1017 CG12171-RA 651..835 608..792 445 82.7 Plus
CG12171-RA 1017 CG12171-RA 296..496 253..453 390 79.6 Plus
CG31548-RA 946 CG31548-RA 395..504 383..492 265 82.7 Plus
CG12171-RA 1017 CG12171-RA 507..628 464..585 205 77.8 Plus
CG31548-RA 946 CG31548-RA 293..381 281..369 175 79.7 Plus
Blast to d_melanogaster_OreR.fa performed 2019-03-16 12:01:56
Subject Length Description Subject Range Query Range Score Percent Strand
chr3R 27901430 chr3R 1458029..1458979 1..951 4755 100 Plus
chr3R 27901430 chr3R 1459359..1460116 35..792 970 75.2 Plus
chr3R 27901430 chr3R 1461700..1462211 792..281 880 78.1 Minus
chr3R 27901430 chr3R 1460483..1460800 742..425 390 74.8 Minus
Blast to dmel-all-all_noncoding-r5.12.fasta performed on 2010-04-22 19:39:50 has no hits.
Blast to na_all.dmel.RELEASE6 performed 2019-03-16 12:01:54
Subject Length Description Subject Range Query Range Score Percent Strand
3R 32079331 3R 5632371..5633325 1..955 4775 100 Plus
3R 32079331 3R 5633701..5634458 35..792 970 75.2 Plus
3R 32079331 3R 5636042..5636553 792..281 880 78.1 Minus
3R 32079331 3R 5634825..5635142 742..425 390 74.8 Minus
Blast to na_arms.dmel.RELEASE6 performed 2011-12-12 20:49:47
Subject Length Description Subject Range Query Range Score Percent Strand
3R 31820162 3R 5373202..5374156 1..955 4775 100 Plus
3R 31820162 3R 5376873..5377054 792..611 520 85.7 Minus
3R 31820162 3R 5375105..5375289 608..792 445 82.7 Plus
3R 31820162 3R 5374750..5374950 253..453 390 79.6 Plus
3R 31820162 3R 5377173..5377282 492..383 265 82.7 Minus
3R 31820162 3R 5375809..5375973 589..425 210 75.1 Minus
3R 31820162 3R 5374961..5375082 464..585 205 77.8 Plus
3R 31820162 3R 5375656..5375706 742..692 180 90.1 Minus
3R 31820162 3R 5377296..5377384 369..281 175 79.7 Minus
Blast to na_te.dros performed 2019-03-16 12:01:55
Subject Length Description Subject Range Query Range Score Percent Strand
Rt1c 5443 Rt1c RT1C 5443bp 4363..4406 742..698 114 78.3 Minus
gypsy7 5486 gypsy7 GYPSY7 5486bp 1955..2019 848..913 111 65.2 Plus

LD44221.complete Sim4 Records

Sim4 to d_melanogaster_OreR.fa performed 2019-03-16 12:02:48 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
chr3R 1458029..1458979 1..951 100   Plus
Sim4 to dmel-all-CDS-r5.12.fasta performed 2008-12-08 19:27:14 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
CG31549-RA 1..774 26..799 100   Plus
Sim4 to dmel-all-CDS-r5.32.fasta performed 2011-03-16 18:26:33 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
CG31549-RA 1..774 26..799 100   Plus
Sim4 to dmel-all-CDS-r5.52.fasta performed 2013-08-04 10:49:02 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
CG31549-RA 1..774 26..799 100   Plus
Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-21 19:01:44 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
CG31549-RA 1..774 26..799 100   Plus
Sim4 to dmel-all-CDS-r6.02.fasta performed 2014-11-27 10:34:50 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
CG31549-RA 1..774 26..799 100   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2008-11-10 21:53:06 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
CG31549-RA 180..1130 1..951 100   Plus
Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-03-16 18:26:33 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
CG31549-RA 180..1130 1..951 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 10:49:02 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
CG31549-RA 180..1130 1..951 100   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-21 19:01:45 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
CG31549-RA 180..1130 1..951 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-27 10:34:50 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
CG31549-RA 180..1130 1..951 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 12:02:48 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
3R 5632371..5633321 1..951 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 12:02:48 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
3R 5632371..5633321 1..951 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 12:02:48 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
3R 5632371..5633321 1..951 100   Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 10:49:02 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3R 1458093..1459043 1..951 100   Plus
Sim4 to na_arms.dmel.RELEASE6 performed 2011-12-09 15:34:53 Download gff for LD44221.complete
Subject Subject Range Query Range Percent Splice Strand
3R 5373202..5374152 1..951 100   Plus

LD44221.pep Sequence

Translation from 25 to 798

> LD44221.pep
MSSFKDKVIIVTGASSGIGASAAVHLAKLGGLLVIVGRNEEKLKETADNI
VAAGGATPLELQADMTKEAEVQQIVGATLAKHGRIDVLVNNAGILETGSI
EATSLEQFDRLMNTNVRSLYQLTMLATPELVKTKGNIVNVSSVCGLRAFP
GVLAYNVSKAAVDQFTACIALELAPKGVRVNAVNPGVIVTDIHKRGGMDE
ETYAKFLEHCKITHALGRPGDVKEVAAAIAFLASDQASFTTGISLPVDGG
RHAMCPR*

LD44221.pep Blast Records

Blast to dana-all-translation-r1.3.fasta performed 2019-03-15 21:54:09
Subject Length Description Subject Range Query Range Score Percent Strand
Dana\GF16338-PA 257 GF16338-PA 1..257 1..257 1212 89.5 Plus
Dana\GF16339-PA 257 GF16339-PA 1..257 1..257 1085 79.8 Plus
Dana\GF18656-PA 256 GF18656-PA 2..256 3..257 937 67.5 Plus
Dana\GF22046-PA 251 GF22046-PA 2..251 3..257 645 53.7 Plus
Dana\GF18657-PA 264 GF18657-PA 11..263 4..256 582 48 Plus
Blast to dere-all-translation-r1.3.fasta performed 2019-03-15 21:54:10
Subject Length Description Subject Range Query Range Score Percent Strand
Dere\GG12965-PA 257 GG12965-PA 1..257 1..257 1296 95.7 Plus
Dere\GG12970-PA 257 GG12970-PA 1..257 1..257 1118 82.1 Plus
Dere\GG10914-PA 256 GG10914-PA 2..256 3..257 944 67.8 Plus
Dere\GG12728-PA 251 GG12728-PA 2..251 3..257 656 54.5 Plus
Dere\GG10919-PA 264 GG10919-PA 11..264 4..257 643 55.7 Plus
Blast to dgri-all-translation-r1.3.fasta performed 2019-03-15 21:54:11
Subject Length Description Subject Range Query Range Score Percent Strand
Dgri\GH22973-PA 257 GH22973-PA 1..257 1..257 1122 81.7 Plus
Dgri\GH22984-PA 257 GH22984-PA 1..257 1..257 1080 78.2 Plus
Dgri\GH17517-PA 257 GH17517-PA 2..257 3..257 846 62.5 Plus
Dgri\GH17658-PA 242 GH17658-PA 2..238 1..250 347 36 Plus
Dgri\GH15276-PA 325 GH15276-PA 74..320 1..250 284 33.3 Plus
Blast to dmel-all-translation-r6.23.fasta performed 2019-03-25 10:21:15
Subject Length Description Subject Range Query Range Score Percent Strand
CG31549-PB 257 CG31549-PB 1..257 1..257 1281 100 Plus
CG31549-PA 257 CG31549-PA 1..257 1..257 1281 100 Plus
CG12171-PA 257 CG12171-PA 1..257 1..257 1058 80.9 Plus
CG31548-PA 256 CG31548-PA 2..256 3..257 914 69 Plus
CG31546-PA 264 CG31546-PA 11..264 4..257 706 56.5 Plus
CG3699-PA 251 CG3699-PA 2..251 3..257 642 53.3 Plus
CG7322-PC 242 CG7322-PC 8..238 7..250 326 35.7 Plus
CG7322-PB 242 CG7322-PB 8..238 7..250 326 35.7 Plus
CG7322-PA 242 CG7322-PA 8..238 7..250 326 35.7 Plus
CG10672-PA 317 CG10672-PA 66..315 1..253 284 33.3 Plus
CG3603-PB 249 CG3603-PB 9..247 7..250 266 31 Plus
CG3603-PA 249 CG3603-PA 9..247 7..250 266 31 Plus
CG7601-PA 326 CG7601-PA 54..275 7..216 233 28.8 Plus
CG6012-PA 308 CG6012-PA 54..238 11..193 217 33.9 Plus
scu-PA 255 CG7113-PA 3..234 5..229 208 28.6 Plus
CG13284-PE 325 CG13284-PE 60..245 10..193 207 31.6 Plus
CG13284-PA 325 CG13284-PA 60..245 10..193 207 31.6 Plus
CG13284-PC 338 CG13284-PC 73..258 10..193 207 31.6 Plus
CG13284-PD 339 CG13284-PD 74..259 10..193 207 31.6 Plus
CG13284-PB 339 CG13284-PB 74..259 10..193 207 31.6 Plus
antdh-PA 250 CG1386-PA 1..203 1..192 206 29.6 Plus
CG31809-PC 316 CG31809-PC 52..236 10..193 204 32.8 Plus
CG31809-PB 316 CG31809-PB 52..236 10..193 204 32.8 Plus
CG31810-PA 324 CG31810-PA 60..244 10..193 204 32.8 Plus
CG40485-PC 247 CG40485-PC 1..203 1..192 195 26.6 Plus
CG40485-PB 247 CG40485-PB 1..203 1..192 195 26.6 Plus
CG5590-PA 412 CG5590-PA 10..197 7..185 195 29.1 Plus
CG31937-PA 321 CG31937-PA 41..237 1..191 191 30.2 Plus
CG10962-PB 249 CG10962-PB 1..213 1..208 187 26.6 Plus
CG17121-PA 361 CG17121-PA 93..260 7..173 185 26.8 Plus
CG40486-PC 247 CG40486-PC 1..207 1..196 184 28.5 Plus
CG40486-PB 247 CG40486-PB 1..207 1..196 184 28.5 Plus
Ldsdh1-PA 320 CG2254-PA 59..250 7..194 184 28 Plus
sro-PA 335 CG12068-PA 28..220 8..191 184 31.1 Plus
CG30491-PB 331 CG30491-PB 46..255 7..204 182 29.2 Plus
CG30491-PA 331 CG30491-PA 46..255 7..204 182 29.2 Plus
spidey-PA 321 CG1444-PA 56..245 10..194 181 28.9 Plus
firl-PE 318 CG14946-PE 56..223 7..173 180 28.6 Plus
firl-PC 318 CG14946-PC 56..223 7..173 180 28.6 Plus
firl-PD 318 CG14946-PD 56..223 7..173 180 28.6 Plus
rdhB-PB 248 CG7077-PB 7..200 4..191 179 28.1 Plus
rdhB-PA 248 CG7077-PA 7..200 4..191 179 28.1 Plus
CG9150-PB 251 CG9150-PB 1..203 1..192 178 28.1 Plus
CG9360-PA 251 CG9360-PA 1..203 1..192 177 31 Plus
CG2064-PA 330 CG2064-PA 44..255 7..206 177 25.1 Plus
CG2065-PB 300 CG2065-PB 15..227 7..207 176 25.6 Plus
CG2065-PA 300 CG2065-PA 15..227 7..207 176 25.6 Plus
CG40485-PA 231 CG40485-PA 1..182 1..173 175 26.9 Plus
Pdh-PC 261 CG4899-PC 2..189 3..192 174 27.6 Plus
Pdh-PA 278 CG4899-PA 19..206 3..192 174 27.6 Plus
Pdh-PD 260 CG4899-PD 2..188 3..192 173 27.5 Plus
Blast to dmoj-all-translation-r1.3.fasta performed 2019-03-15 21:54:11
Subject Length Description Subject Range Query Range Score Percent Strand
Dmoj\GI10241-PA 257 GI10241-PA 1..257 1..257 1086 77.4 Plus
Dmoj\GI10240-PA 257 GI10240-PA 1..257 1..257 1084 82.5 Plus
Dmoj\GI22521-PA 256 GI22521-PA 2..256 3..257 944 68.2 Plus
Dmoj\GI14997-PA 255 GI14997-PA 2..255 3..257 660 53.5 Plus
Dmoj\GI21510-PA 242 GI21510-PA 2..238 1..250 318 34 Plus
Blast to dper-all-translation-r1.3.fasta performed 2019-03-15 21:54:12
Subject Length Description Subject Range Query Range Score Percent Strand
Dper\GL24510-PA 257 GL24510-PA 1..257 1..257 1190 87.9 Plus
Dper\GL24511-PA 257 GL24511-PA 1..257 1..257 1113 80.9 Plus
Dper\GL23021-PA 256 GL23021-PA 2..256 3..257 970 69.8 Plus
Dper\GL14417-PA 255 GL14417-PA 2..255 3..257 646 52.7 Plus
Dper\GL21318-PA 350 GL21318-PA 110..346 1..250 350 36 Plus
Blast to dpse-all-translation-r3.2.fasta performed 2019-03-15 21:54:12
Subject Length Description Subject Range Query Range Score Percent Strand
Dpse\GA27306-PA 257 GA27306-PA 1..257 1..257 1187 87.5 Plus
Dpse\GA11451-PA 257 GA11451-PA 1..257 1..257 1122 81.7 Plus
Dpse\GA16317-PA 256 GA16317-PA 2..256 3..257 968 69.8 Plus
Dpse\GA17622-PA 255 GA17622-PA 2..255 3..257 646 52.7 Plus
Dpse\GA20258-PA 242 GA20258-PA 2..238 1..250 350 36 Plus
Blast to dsec-all-translation-r1.3.fasta performed 2019-03-15 21:54:13
Subject Length Description Subject Range Query Range Score Percent Strand
Dsec\GM10825-PA 257 GM10825-PA 1..257 1..257 1311 97.7 Plus
Dsec\GM10826-PA 257 GM10826-PA 1..257 1..257 1118 82.1 Plus
Dsec\GM10606-PA 256 GM10606-PA 2..256 3..257 952 68.6 Plus
Dsec\GM19006-PA 251 GM19006-PA 2..251 3..257 650 53.3 Plus
Dsec\GM10607-PA 264 GM10607-PA 11..264 4..257 645 56.5 Plus
Blast to dsim-all-translation-r1.4.fasta performed 2019-03-15 21:54:13
Subject Length Description Subject Range Query Range Score Percent Strand
Dsim\GD19803-PA 257 GD19803-PA 1..257 1..257 1112 82.1 Plus
Dsim\GD19595-PA 256 GD19595-PA 2..256 3..257 952 68.6 Plus
Dsim\GD16445-PA 251 GD16445-PA 2..251 3..257 650 53.3 Plus
Dsim\GD19596-PA 264 GD19596-PA 11..264 4..257 645 56.5 Plus
Dsim\GD19802-PA 1449 GD19802-PA 1351..1449 159..257 533 97 Plus
Blast to dvir-all-translation-r1.2.fasta performed 2019-03-15 21:54:14
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\GJ11045-PA 257 GJ11045-PA 1..257 1..257 1096 80.9 Plus
Dvir\GJ11044-PA 257 GJ11044-PA 1..257 1..257 1078 83.7 Plus
Dvir\GJ10125-PA 255 GJ10125-PA 2..255 3..257 930 69 Plus
Dvir\GJ10124-PA 255 GJ10124-PA 2..255 3..257 891 64.7 Plus
Dvir\GJ15354-PA 255 GJ15354-PA 2..255 3..257 653 53.9 Plus
Blast to dwil-all-translation-r1.3.fasta performed 2019-03-15 21:54:14
Subject Length Description Subject Range Query Range Score Percent Strand
Dwil\GK13927-PA 257 GK13927-PA 1..257 1..257 1186 86.4 Plus
Dwil\GK13928-PA 257 GK13928-PA 1..257 1..257 1061 77 Plus
Dwil\GK13365-PA 256 GK13365-PA 2..256 3..257 855 66.7 Plus
Dwil\GK10169-PA 255 GK10169-PA 2..255 3..257 643 53.1 Plus
Dwil\GK20088-PA 242 GK20088-PA 2..238 1..250 373 36.8 Plus
Blast to dyak-all-translation-r1.3.fasta performed 2019-03-15 21:54:15
Subject Length Description Subject Range Query Range Score Percent Strand
Dyak\GE10146-PA 257 GE10146-PA 1..257 1..257 1265 93.4 Plus
Dyak\GE10147-PA 257 GE10147-PA 1..257 1..257 1108 81.3 Plus
Dyak\GE24152-PA 256 GE24152-PA 2..256 3..257 964 69.4 Plus
Dyak\GE24153-PA 264 GE24153-PA 11..264 4..257 643 56.5 Plus
Dyak\GE16554-PA 251 GE16554-PA 2..251 3..257 639 53.3 Plus

LD44221.hyp Sequence

Translation from 25 to 798

> LD44221.hyp
MSSFKDKVIIVTGASSGIGASAAVHLAKLGGLLVIVGRNEEKLKETADNI
VAAGGATPLELQADMTKEAEVQQIVGATLAKHGRIDVLVNNAGILETGSI
EATSLEQFDRLMNTNVRSLYQLTMLATPELVKTKGNIVNVSSVCGLRAFP
GVLAYNVSKAAVDQFTACIALELAPKGVRVNAVNPGVIVTDIHKRGGMDE
ETYAKFLEHCKITHALGRPGDVKEVAAAIAFLASDQASFTTGISLPVDGG
RHAMCPR*

LD44221.hyp Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 17:14:33
Subject Length Description Subject Range Query Range Score Percent Strand
CG31549-PB 257 CG31549-PB 1..257 1..257 1281 100 Plus
CG31549-PA 257 CG31549-PA 1..257 1..257 1281 100 Plus
CG12171-PA 257 CG12171-PA 1..257 1..257 1058 80.9 Plus
CG31548-PA 256 CG31548-PA 2..256 3..257 914 69 Plus
CG31546-PA 264 CG31546-PA 11..264 4..257 706 56.5 Plus