Clone RE30523 Report

Search the DGRC for RE30523

Clone and Library Details

Library:RE
Tissue Source:Drosophila melanogaster embryo
Created by:Piero Carninci, RIKEN Genome Science Laboratory
Date Registered:2000-10-23
Comments:Average reported size from P Carninci is 2.3kb. Directionally cloned:5 end at XhoI, 3 end at BamHI
Original Plate Number:305
Well:23
Vector:pFlc-1
Associated Gene/TranscriptSep1-RA
Protein status:RE30523.pep: gold
Preliminary Size:2006
Sequenced Size:2054

Associated Genes

Associations are from manual ordering of a clone or by a periodic analysis.
Gene Date Evidence
CG1403 2002-01-01 Sim4 clustering to Release 2
CG1403 2002-06-10 Blastp of sequenced clone
CG1403 2003-01-01 Sim4 clustering to Release 3
Sep1 2008-04-29 Release 5.5 accounting
Sep1 2008-08-15 Release 5.9 accounting
Sep1 2008-12-18 5.12 accounting

Clone Sequence Records

RE30523.complete Sequence

2054 bp (2054 high quality bases) assembled on 2002-06-10

GenBank Submission: AY121679

> RE30523.complete
AGCAGGATTTGCCAGTTGCGCGCTGCGGTCACACTAGTTGCCCTGCTATT
TTGTTCGTCATTACGTTGATTTCTTCGTTCGTTTCGTGTTTTTTTTTTTA
AAATGGCCGATACAAAGGGCTTTTCCAGCATCGAGACGCCCGGCTATGTG
GGCTTCGCCAACCTGCCCAACCAGGTGCATCGAAAGTCCGTGAAAAAGGG
ATTCGAGTTCACCCTAATGGTGGTGGGCGAGTCCGGACTGGGCAAGTCCA
CGCTGGTCAATAGTCTCTTTCTCACCGATCTCTATCCCGAGCGCATTATT
CCGGACGCCATAGAGAAACAAAAGCAAACCGTAAAGCTGGAGGCATCGAC
GGTGGAGATCGAGGAGCGCGGCGTCAAGCTAAGATTGACGGTGGTGGACA
CACCCGGATTCGGCGATGCCATCGACAACTCCAACAGCTTTGGTGCCATA
CTCGAGTACATCGATGAGCAATACGAGCGCTTCCTGCGCGACGAGAGCGG
CCTGAACAGACGCAACATTGTGGACAATCGCATTCACTGCTGTTTCTACT
TTATATCGCCGTTCGGTCATGGCCTAAAACCCCTGGACGTAGAGTTCATG
AAGAAGCTGCACTCTAAAGTTAACATTGTGCCCGTGATCGCAAAGGCCGA
TTGTCTCACAAAGAAGGAGATCCTGCGCCTGAAGTGCCGCATTATGCAGG
AGATCGAGAGCCACGGCATCAAGATCTACCCACTGCCAGACTGTGATTCC
GATGAGGACGAGGACTACAAGGAGCAGGTGAAGCAACTTAAGGAAGCTGT
GCCTTTCGCCGTCTGCGGCGCCAACACACTGCTCGAGGTCAAGGGCAAGA
AGGTGCGCGGTCGTCTGTATCCATGGGGCGTGGTCGAGGTAGAGAATCCC
GATCACTGCGACTTCATCAAGCTGCGCACGATGCTCATCACCCACATGCA
GGACCTGCAGGAGGTCACGCAGGAGGTGCACTACGAAAACTACCGCTCCG
ACCGCCTCGCCAAGGGCATCAAGGGCAAGGAGAACGGCGTGAAGGCCGAG
CGGGACAGCAGCTCGCAGGTGGTGTCGAACAGCGTGCTCGGCGAGAAGGA
CCGCATCCTGCAAGAGAAGGAGGCCGAGCTGCGGCGCATGCAGGAGATGC
TCGCCCAGATGCAGGCGCGCATGCAGGCCCAGCAGTGAGCAGGCGGGTAT
ATCTTTTATATACGCGCTTGGCGGAGGCGGCAATCTCTAGTTATACACAA
GTCGTATATATACACATATACAAAACATATATTTTGTACGCAGATTTTGG
TTGCGATCGCGCTTTAATTCTACTCTCAATTGTGAAACGACGGGCGCTAA
GTATTCTGTATTAATTTCGCATTCTCCATATATATATATCCGTAGTATCT
AGATATACACACCACATACATTTATACACACATCTAACTTACACCTGCCG
CGTATGCAAATGCCTGCAATGCAATTGCTTCATCAGAAAAACAAAGAACA
TAAGGAACACCAACCATCAGCGAACTCATATCACTTCTATCAAGCGAAAC
CACACACGAAAACAATTGTATTCCTATTATGTATTTAGACGCGATTAACA
ATGAATTATTATTGTAATACGCATCTAGGCTAAGCGAATCCAATCCGATT
TATATTGTATGTTAATTCATTTTTGTCTGTTTGCCTTACCCTTATCTGTG
TTGTCTCAAAGCGAGCAATTTATTTAGGTTATACTATATGTTGCGTGTCA
GCCTGCCCTTCCGATCCTTCTTGACCTCCATGGTCCGCTGTCCTGCAGTG
CCGCCCGATTCCTAAGCACGATCTGATTGAATAAACAGTGATCCCTTCCT
AGGCGGTCCCGGGAACCCATGGCCAGTTGCGGAAACTCGGTTTAGCAGGC
TACTCGCTTAAAAGTGCACTCCTTTAACGGATGAAACAAAAACCAAAATG
TGAAATCTCTTTTATTATGTATATGAAGACTTTTTTAACTCAATATAATC
CTCTATGAATATATAAATATGCTTTTTATACAATTTGCAAAAAAAAAAAA
AAAA

RE30523.complete Blast Records

Blast to MB8.fasta performed 2010-07-15 18:50:24
Subject Length Description Subject Range Query Range Score Percent Strand
Sep1.b 2364 Sep1.b 40..2074 1..2037 10115 99.8 Plus
Sep1-RA 2095 Sep1-RA 40..2074 1..2037 10115 99.8 Plus
Sep1.a 2040 Sep1.a 123..2039 121..2037 9570 99.9 Plus
Blast to d_melanogaster_OreR.fa performed 2019-03-16 21:07:59
Subject Length Description Subject Range Query Range Score Percent Strand
chrX 22417052 chrX 21225747..21226845 939..2037 5480 99.9 Plus
chrX 22417052 chrX 21225313..21225679 572..938 1835 100 Plus
chrX 22417052 chrX 21224927..21225187 312..572 1305 100 Plus
chrX 22417052 chrX 21224670..21224862 121..313 965 100 Plus
chrX 22417052 chrX 21223434..21223551 1..120 535 98.3 Plus
chrX 22417052 chrX 16576255..16576364 284..175 340 87.3 Minus
chr2R 21145070 chr2R 4125908..4126028 140..260 335 85.1 Plus
chrX 22417052 chrX 16575211..16575534 929..606 270 72.2 Minus
chr2R 21145070 chr2R 4126130..4126227 362..459 205 80.6 Plus
Blast to dmel-all-all_noncoding-r5.12.fasta performed on 2010-04-22 20:48:30 has no hits.
Blast to na_all.dmel.RELEASE6 performed 2019-03-16 21:07:57
Subject Length Description Subject Range Query Range Score Percent Strand
X 23542271 X 21360589..21361687 939..2037 5480 99.9 Plus
X 23542271 X 21360155..21360521 572..938 1835 100 Plus
X 23542271 X 21359769..21360029 312..572 1305 100 Plus
X 23542271 X 21359512..21359704 121..313 965 100 Plus
X 23542271 X 21358276..21358393 1..120 535 98.3 Plus
X 23542271 X 16686598..16686707 284..175 340 87.3 Minus
2R 25286936 2R 8238415..8238535 140..260 320 84.3 Plus
X 23542271 X 16685554..16685877 929..606 270 72.2 Minus
2R 25286936 2R 8238637..8238734 362..459 205 80.6 Plus
X 23542271 X 16686459..16686534 423..348 200 84.2 Minus
Blast to na_arms.dmel.RELEASE6 performed 2011-12-12 20:23:04
Subject Length Description Subject Range Query Range Score Percent Strand
X 23527363 X 21345681..21346779 939..2037 5480 99.9 Plus
X 23527363 X 21345247..21345613 572..938 1835 100 Plus
X 23527363 X 21344861..21345121 312..572 1305 100 Plus
X 23527363 X 21344604..21344796 121..313 965 100 Plus
X 23527363 X 21343368..21343485 1..120 545 98.3 Plus
X 23527363 X 16694696..16694805 284..175 340 87.2 Minus
2R 25260384 2R 8239614..8239734 140..260 320 84.2 Plus
X 23527363 X 16693652..16693776 929..805 235 79.2 Minus
2R 25260384 2R 8239836..8239933 362..459 205 80.6 Plus
X 23527363 X 16694557..16694632 423..348 200 84.2 Minus
X 23527363 X 16694429..16694491 551..489 150 82.5 Minus
Blast to na_te.dros performed on 2019-03-16 21:07:57 has no hits.

RE30523.complete Sim4 Records

Sim4 to d_melanogaster_OreR.fa performed 2019-03-16 21:09:00 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
chrX 21223434..21223551 1..120 98 -> Plus
chrX 21224670..21224862 121..313 100 -> Plus
chrX 21224929..21225187 314..572 100 -> Plus
chrX 21225314..21225679 573..938 100 -> Plus
chrX 21225747..21226845 939..2038 99   Plus
Sim4 to dmel-all-CDS-r5.12.fasta performed 2008-12-08 20:08:40 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
Sep1-RA 1..1086 103..1188 100   Plus
Sim4 to dmel-all-CDS-r5.32.fasta performed 2011-03-16 17:26:20 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
Sep1-RA 1..1086 103..1188 100   Plus
Sim4 to dmel-all-CDS-r5.52.fasta performed 2013-08-04 17:33:25 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
Sep1-RA 1..1086 103..1188 100   Plus
Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-21 18:16:48 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
Sep1-RA 1..1086 103..1188 100   Plus
Sim4 to dmel-all-CDS-r6.02.fasta performed 2014-11-27 16:07:03 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
Sep1-RA 1..1086 103..1188 100   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2008-11-10 20:54:37 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
Sep1-RA 1..2035 1..2037 99   Plus
Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-03-16 17:26:20 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
Sep1-RA 1..2035 1..2038 99   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 17:33:25 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
Sep1-RA 1..2035 1..2038 99   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-21 18:16:48 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
Sep1-RA 1..2035 1..2037 99   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-27 16:07:03 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
Sep1-RA 1..2025 11..2038 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 21:09:00 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
X 21358276..21358393 1..120 98 -> Plus
X 21359512..21359704 121..313 100 -> Plus
X 21359771..21360029 314..572 100 -> Plus
X 21360156..21360521 573..938 100 -> Plus
X 21360589..21361687 939..2038 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 21:09:00 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
X 21358276..21358393 1..120 98 -> Plus
X 21359512..21359704 121..313 100 -> Plus
X 21359771..21360029 314..572 100 -> Plus
X 21360156..21360521 573..938 100 -> Plus
X 21360589..21361687 939..2038 99   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2019-03-16 21:09:00 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
X 21358276..21358393 1..120 98 -> Plus
X 21359512..21359704 121..313 100 -> Plus
X 21359771..21360029 314..572 100 -> Plus
X 21360156..21360521 573..938 100 -> Plus
X 21360589..21361687 939..2038 99   Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 17:33:25 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
arm_X 21229303..21229420 1..120 98 -> Plus
arm_X 21230539..21230731 121..313 100 -> Plus
arm_X 21230798..21231056 314..572 100 -> Plus
arm_X 21231183..21231548 573..938 100 -> Plus
arm_X 21231616..21232714 939..2038 99   Plus
Sim4 to na_arms.dmel.RELEASE6 performed 2011-12-09 14:50:07 Download gff for RE30523.complete
Subject Subject Range Query Range Percent Splice Strand
X 21344863..21345121 314..572 100 -> Plus
X 21345248..21345613 573..938 100 -> Plus
X 21345681..21346779 939..2038 99   Plus
X 21343368..21343485 1..120 98 -> Plus
X 21344604..21344796 121..313 100 -> Plus

RE30523.hyp Sequence

Translation from 102 to 1187

> RE30523.hyp
MADTKGFSSIETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKST
LVNSLFLTDLYPERIIPDAIEKQKQTVKLEASTVEIEERGVKLRLTVVDT
PGFGDAIDNSNSFGAILEYIDEQYERFLRDESGLNRRNIVDNRIHCCFYF
ISPFGHGLKPLDVEFMKKLHSKVNIVPVIAKADCLTKKEILRLKCRIMQE
IESHGIKIYPLPDCDSDEDEDYKEQVKQLKEAVPFAVCGANTLLEVKGKK
VRGRLYPWGVVEVENPDHCDFIKLRTMLITHMQDLQEVTQEVHYENYRSD
RLAKGIKGKENGVKAERDSSSQVVSNSVLGEKDRILQEKEAELRRMQEML
AQMQARMQAQQ*

RE30523.hyp Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 14:59:17
Subject Length Description Subject Range Query Range Score Percent Strand
Sep1-PA 361 CG1403-PA 1..361 1..361 1861 100 Plus
Sep4-PG 375 CG9699-PG 13..359 15..358 1096 59.5 Plus
Sep4-PC 427 CG9699-PC 65..411 15..358 1096 59.5 Plus
Sep4-PE 427 CG9699-PE 65..411 15..358 1096 59.5 Plus
Sep4-PD 427 CG9699-PD 65..411 15..358 1096 59.5 Plus

RE30523.pep Sequence

Translation from 102 to 1187

> RE30523.pep
MADTKGFSSIETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKST
LVNSLFLTDLYPERIIPDAIEKQKQTVKLEASTVEIEERGVKLRLTVVDT
PGFGDAIDNSNSFGAILEYIDEQYERFLRDESGLNRRNIVDNRIHCCFYF
ISPFGHGLKPLDVEFMKKLHSKVNIVPVIAKADCLTKKEILRLKCRIMQE
IESHGIKIYPLPDCDSDEDEDYKEQVKQLKEAVPFAVCGANTLLEVKGKK
VRGRLYPWGVVEVENPDHCDFIKLRTMLITHMQDLQEVTQEVHYENYRSD
RLAKGIKGKENGVKAERDSSSQVVSNSVLGEKDRILQEKEAELRRMQEML
AQMQARMQAQQ*

RE30523.pep Blast Records

Blast to dana-all-translation-r1.3.fasta performed 2019-03-16 06:18:06
Subject Length Description Subject Range Query Range Score Percent Strand
Dana\GF20920-PA 364 GF20920-PA 1..364 1..361 1829 95.9 Plus
Dana\GF22296-PA 375 GF22296-PA 13..359 15..358 1132 59.5 Plus
Dana\GF12347-PA 545 GF12347-PA 123..416 10..303 939 59.3 Plus
Dana\GF18330-PA 419 GF18330-PA 17..346 9..346 728 44.2 Plus
Dana\GF11226-PA 419 GF11226-PA 21..349 10..346 697 42.6 Plus
Blast to dere-all-translation-r1.3.fasta performed 2019-03-16 06:18:06
Subject Length Description Subject Range Query Range Score Percent Strand
Dere\GG19725-PA 361 GG19725-PA 1..361 1..361 1930 100 Plus
Dere\GG18234-PA 430 GG18234-PA 68..414 15..358 1125 59.5 Plus
Dere\GG23345-PA 541 GG23345-PA 119..413 10..304 939 59.1 Plus
Dere\GG15324-PA 419 GG15324-PA 17..346 9..346 730 44.5 Plus
Dere\GG10692-PA 422 GG10692-PA 15..349 4..346 701 42.5 Plus
Blast to dgri-all-translation-r1.3.fasta performed 2019-03-16 06:18:07
Subject Length Description Subject Range Query Range Score Percent Strand
Dgri\GH24812-PA 362 GH24812-PA 1..362 1..361 1851 95.6 Plus
Dgri\GH24231-PA 440 GH24231-PA 78..424 15..358 1121 58.6 Plus
Dgri\GH21082-PA 541 GH21082-PA 119..413 10..304 946 59.1 Plus
Dgri\GH16156-PA 419 GH16156-PA 17..346 9..346 727 44.5 Plus
Blast to dmel-all-translation-r6.23.fasta performed 2019-03-25 10:32:07
Subject Length Description Subject Range Query Range Score Percent Strand
Sep1-PA 361 CG1403-PA 1..361 1..361 1861 100 Plus
Sep4-PG 375 CG9699-PG 13..359 15..358 1096 59.5 Plus
Sep4-PC 427 CG9699-PC 65..411 15..358 1096 59.5 Plus
Sep4-PE 427 CG9699-PE 65..411 15..358 1096 59.5 Plus
Sep4-PD 427 CG9699-PD 65..411 15..358 1096 59.5 Plus
Sep4-PB 427 CG9699-PB 65..411 15..358 1096 59.5 Plus
Sep4-PF 427 CG9699-PF 65..411 15..358 1096 59.5 Plus
Sep4-PA 427 CG9699-PA 65..411 15..358 1096 59.5 Plus
pnut-PA 539 CG8705-PA 117..451 10..353 906 53 Plus
pnut-PB 539 CG8705-PB 117..451 10..353 906 53 Plus
Sep2-PA 419 CG4173-PA 17..346 9..346 703 44.2 Plus
Sep5-PB 422 CG2916-PB 21..349 10..346 663 42.6 Plus
Sep5-PA 422 CG2916-PA 21..349 10..346 663 42.6 Plus
Sep2-PB 360 CG4173-PB 20..287 76..346 588 45.2 Plus
Blast to dmoj-all-translation-r1.3.fasta performed 2019-03-16 06:18:07
Subject Length Description Subject Range Query Range Score Percent Strand
Dmoj\GI21539-PA 364 GI21539-PA 1..364 1..361 1851 95.3 Plus
Dmoj\GI18682-PA 541 GI18682-PA 119..413 10..304 951 59.5 Plus
Dmoj\GI10938-PA 294 GI10938-PA 1..278 84..358 860 56.6 Plus
Dmoj\GI22174-PA 419 GI22174-PA 17..346 9..346 729 44.8 Plus
Blast to dper-all-translation-r1.3.fasta performed 2019-03-16 06:18:08
Subject Length Description Subject Range Query Range Score Percent Strand
Dper\GL16012-PA 363 GL16012-PA 1..363 1..361 1855 95.6 Plus
Dper\GL19764-PA 369 GL19764-PA 39..353 31..358 997 56.7 Plus
Dper\GL11211-PA 534 GL11211-PA 112..406 10..304 950 59.5 Plus
Dper\GL13925-PA 419 GL13925-PA 17..346 9..346 728 44.2 Plus
Dper\GL10608-PA 421 GL10608-PA 17..345 10..346 676 41.4 Plus
Blast to dpse-all-translation-r3.2.fasta performed 2019-03-16 06:18:08
Subject Length Description Subject Range Query Range Score Percent Strand
Dpse\GA12712-PA 363 GA12712-PA 1..363 1..361 1855 95.6 Plus
Dpse\GA21972-PA 432 GA21972-PA 70..416 15..358 1121 59.2 Plus
Dpse\GA21269-PA 534 GA21269-PA 112..406 10..304 950 59.5 Plus
Dpse\GA18006-PA 419 GA18006-PA 17..346 9..346 728 44.2 Plus
Dpse\GA15516-PA 421 GA15516-PA 17..345 10..346 677 41.4 Plus
Blast to dsec-all-translation-r1.3.fasta performed 2019-03-16 06:18:09
Subject Length Description Subject Range Query Range Score Percent Strand
Dsec\GM23080-PA 361 GM23080-PA 1..361 1..361 1925 99.7 Plus
Dsec\GM21017-PA 539 GM21017-PA 117..411 10..304 940 59.1 Plus
Dsec\GM23178-PA 419 GM23178-PA 17..346 9..346 727 44.2 Plus
Dsec\GM20740-PA 422 GM20740-PA 15..349 4..346 695 42.2 Plus
Dsec\GM13376-PA 217 GM13376-PA 1..201 161..358 578 54.5 Plus
Blast to dsim-all-translation-r1.4.fasta performed 2019-03-16 06:18:10
Subject Length Description Subject Range Query Range Score Percent Strand
Dsim\GD15733-PA 375 GD15733-PA 13..359 15..358 1130 59.5 Plus
Dsim\GD10550-PA 528 GD10550-PA 106..400 10..304 939 59.1 Plus
Dsim\GD17531-PA 164 GD17531-PA 1..164 198..361 872 100 Plus
Dsim\GD20051-PA 419 GD20051-PA 17..346 9..346 725 44.2 Plus
Dsim\GD10205-PA 422 GD10205-PA 15..349 4..346 697 42.5 Plus
Blast to dvir-all-translation-r1.2.fasta performed 2019-03-16 06:18:10
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\GJ16012-PA 364 GJ16012-PA 1..364 1..361 1861 95.9 Plus
Dvir\GJ15264-PA 440 GJ15264-PA 85..424 15..358 1061 57.5 Plus
Dvir\GJ21698-PA 538 GJ21698-PA 116..409 10..303 953 59.7 Plus
Dvir\GJ24294-PA 419 GJ24294-PA 17..346 9..346 727 44.5 Plus
Blast to dwil-all-translation-r1.3.fasta performed 2019-03-16 06:18:11
Subject Length Description Subject Range Query Range Score Percent Strand
Dwil\GK25618-PA 364 GK25618-PA 1..364 1..361 1812 94 Plus
Dwil\GK10092-PA 431 GK10092-PA 69..415 15..358 1133 59.8 Plus
Dwil\GK15820-PA 546 GK15820-PA 124..417 10..303 937 59 Plus
Dwil\GK22714-PA 419 GK22714-PA 17..346 9..346 728 44.2 Plus
Dwil\GK15728-PA 422 GK15728-PA 18..346 10..346 697 42.8 Plus
Blast to dyak-all-translation-r1.3.fasta performed 2019-03-16 06:18:11
Subject Length Description Subject Range Query Range Score Percent Strand
Dyak\GE17923-PA 361 GE17923-PA 1..361 1..361 1930 100 Plus
Dyak\GE15652-PA 430 GE15652-PA 68..414 15..358 1127 59.5 Plus
Dyak\GE19185-PA 541 GE19185-PA 119..413 10..304 939 59.1 Plus
Dyak\Sep2-PA 419 GE25056-PA 17..346 9..346 725 44.2 Plus
Dyak\GE23454-PA 422 GE23454-PA 15..349 4..346 698 42.6 Plus