Clone HL08032 Report

Search the DGRC for HL08032

Clone and Library Details

Library:HL
Tissue Source:Drosophila melanogaster head
Created by:Ling Hong
Date Registered:1997-11-24
Comments:Constructed using Stratagene ZAP-cDNA Synthesis kit. Oligo dT-primed and directionally cloned at EcoRI and XhoI in BlueScript SK(+/-)
Original Plate Number:80
Well:32
Vector:pOT2
Associated Gene/Transcriptcbx-RB
Protein status:HL08032.pep: gold
Preliminary Size:1300
Sequenced Size:904

Associated Genes

Associations are from manual ordering of a clone or by a periodic analysis.
Gene Date Evidence
CG10536 2001-07-04 Blastp of sequenced clone
CG10536 2003-01-01 Sim4 clustering to Release 3
cbx 2008-04-29 Release 5.5 accounting
cbx 2008-08-15 Release 5.9 accounting
cbx 2008-12-18 5.12 accounting

Clone Sequence Records

HL08032.complete Sequence

904 bp (904 high quality bases) assembled on 2001-07-04

GenBank Submission: AY051650

> HL08032.complete
TTCAACAAGCAACAAAATTGTCCGTTTGTTTATGTTACTAGTTTTTTGTT
TAATTTTTCTATTGTCCGGACAAAATGACTCTCGATTTGGATGCGCATAA
GAAAGATGACAAGTTGCTGATCACAACCATACAGCAGGAGTATAAAATTC
TGGCCGAATACAAAATGATTGAGTCGGAGAAGTTGAGTGGGATATATGTG
ATACCCAGCTATGCTAACTCCTTGCAGTGGTTTGGAGTCTTCTTTGGCCG
ACAGGGCTTGTATGCGGAAAGTGTTTTTCGATTCACAATTTTGCTCCCAG
ATCGTTTTCCCGACGACAAAAGTCTTCCGAGCATAATTTTCCAGCAGGAT
GTGATTCATCCCCATGTGTGCCCGTACACCCACAGTTTGGACGTGAGCCA
CGCCTTCCCGGAGTGGCGCTGTGGAGAGGATCACCTTTGGCAGCTTTTAA
AGTACCTGCAAGTCATTTTCTCCGATCCCTTGGACAGTATTCGGGGCATT
GAGGTAGATAAGCTCAAAAACAGCGAGGCTGCGGAGCTGCTGATGAATAA
CAAGGAGGAGTATGTTGCTCGCGTACAGGAAAATATCAAGGAGAGCAAGG
AGCATATATTTGACACCCCACCCACCGAAGATCCGCACTACATAGTATTC
GAGAAGTTCCAGCAGGACGTGCACGGGCCCGTTCTGGAGCGCATCAAGGC
CGGAAGAAGCAAGCTGACGGAGCCTTCAGCGCAACAAGCAAATGGTGGAC
ATGCAACTGGTCTGTCATGGGTTAAGGAGGGCGAGTTTAAGCCACTTAGC
ATTGAGTAAAACCAATATACATATATTCTGTAAGTTATATTCTATCGTTG
TCTCAATTTTAAAACGATTAAAGTTATTCAATTAAAAAAAAAAAAAAAAA
AAAA

HL08032.complete Blast Records

Blast to MB8.fasta performed 2010-07-15 20:19:55
Subject Length Description Subject Range Query Range Score Percent Strand
cbx-RB 969 cbx-RB 22..905 1..884 4420 100 Plus
cbx-RA 1038 cbx-RA 253..1031 106..884 3895 100 Plus
CG30010-RA 899 CG30010-RA 812..899 884..797 440 100 Minus
Blast to d_melanogaster_OreR.fa performed 2014-11-27 00:16:21
Subject Length Description Subject Range Query Range Score Percent Strand
chr2R 21145070 chr2R 5751994..5752548 883..329 2775 100 Minus
chr2R 21145070 chr2R 5752902..5753061 160..1 800 100 Minus
chr2R 21145070 chr2R 5752602..5752705 329..226 520 100 Minus
chr2R 21145070 chr2R 5752768..5752832 225..161 325 100 Minus
Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-27 00:16:18
Subject Length Description Subject Range Query Range Score Percent Strand
cbx-RB 735 CG10536-PB 1..735 75..809 3675 100 Plus
cbx-RA 645 CG10536-PA 1..645 165..809 3225 100 Plus
cbx-RC 552 CG10536-PC 1..322 165..504 1395 94.7 Plus
Blast to dmel-all-all_noncoding-r5.12.fasta performed on 2010-04-22 17:29:42 has no hits.
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-27 00:16:16
Subject Length Description Subject Range Query Range Score Percent Strand
cbx-RB 908 CG10536-RB 20..902 1..883 4415 100 Plus
cbx-RA 1036 CG10536-RA 253..1030 106..883 3890 100 Plus
cbx-RC 766 CG10536-RC 72..456 102..504 1710 95.5 Plus
Blast to dmel-all-translation-r6.02.fasta performed 2014-11-27 00:16:20
Subject Length Description Subject Range Query Range Score Percent Strand
cbx-PB 244 CG10536-PB 16..244 120..806 1213 100 Plus
cbx-PA 214 CG10536-PA 1..214 165..806 1139 100 Plus
cbx-PC 183 CG10536-PC 1..183 165..806 937 85.5 Plus
CG16894-PA 266 CG16894-PA 11..211 120..701 492 45.5 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-27 00:16:13
Subject Length Description Subject Range Query Range Score Percent Strand
2R 25286936 2R 9864515..9865070 884..329 2780 100 Minus
2R 25286936 2R 9865424..9865583 160..1 800 100 Minus
2R 25286936 2R 9865124..9865227 329..226 520 100 Minus
2R 25286936 2R 9865290..9865354 225..161 325 100 Minus
Blast to na_arms.dmel.RELEASE6 performed 2011-12-12 21:42:59
Subject Length Description Subject Range Query Range Score Percent Strand
2R 25260384 2R 9865714..9866269 884..329 2780 100 Minus
2R 25260384 2R 9866623..9866782 160..1 800 100 Minus
2R 25260384 2R 9866323..9866426 329..226 520 100 Minus
2R 25260384 2R 9866489..9866553 225..161 325 100 Minus
Blast to na_te.dros performed on 2014-11-27 00:16:14 has no hits.

HL08032.complete Sim4 Records

Sim4 to d_melanogaster_OreR.fa performed 2014-11-27 01:43:49 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
chr2R 5752902..5753061 1..160 100   Minus
chr2R 5752768..5752832 161..225 100 <- Minus
chr2R 5752602..5752705 226..329 100 <- Minus
chr2R 5751994..5752547 330..883 100 <- Minus
Sim4 to dmel-all-CDS-r5.12.fasta performed 2008-12-08 17:13:31 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
cbx-RB 1..735 75..809 100   Plus
Sim4 to dmel-all-CDS-r5.32.fasta performed 2011-03-16 20:03:10 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
cbx-RB 1..735 75..809 100   Plus
Sim4 to dmel-all-CDS-r5.52.fasta performed 2013-08-04 07:07:35 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
cbx-RB 1..735 75..809 100   Plus
Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-21 20:33:44 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
cbx-RB 1..735 75..809 100   Plus
Sim4 to dmel-all-CDS-r6.02.fasta performed 2014-11-27 01:43:49 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
cbx-RB 1..735 75..809 100   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2008-11-10 23:51:44 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
cbx-RB 1..883 1..883 100   Plus
Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-03-16 20:03:09 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
cbx-RB 10..892 1..883 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 07:07:35 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
cbx-RB 20..902 1..883 100   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-21 20:33:44 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
cbx-RB 1..883 1..883 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-27 01:43:49 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
cbx-RB 20..902 1..883 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-27 01:43:49 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
2R 9865424..9865583 1..160 100   Minus
2R 9865290..9865354 161..225 100 <- Minus
2R 9865124..9865227 226..329 100 <- Minus
2R 9864516..9865069 330..883 100 <- Minus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 07:07:35 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
arm_2R 5752929..5753088 1..160 100   Minus
arm_2R 5752795..5752859 161..225 100 <- Minus
arm_2R 5752629..5752732 226..329 100 <- Minus
arm_2R 5752021..5752574 330..883 100 <- Minus
Sim4 to na_arms.dmel.RELEASE6 performed 2011-12-09 17:10:35 Download gff for HL08032.complete
Subject Subject Range Query Range Percent Splice Strand
2R 9866623..9866782 1..160 100   Minus
2R 9866489..9866553 161..225 100 <- Minus
2R 9866323..9866426 226..329 100 <- Minus
2R 9865715..9866268 330..883 100 <- Minus

HL08032.pep Sequence

Translation from 74 to 808

> HL08032.pep
MTLDLDAHKKDDKLLITTIQQEYKILAEYKMIESEKLSGIYVIPSYANSL
QWFGVFFGRQGLYAESVFRFTILLPDRFPDDKSLPSIIFQQDVIHPHVCP
YTHSLDVSHAFPEWRCGEDHLWQLLKYLQVIFSDPLDSIRGIEVDKLKNS
EAAELLMNNKEEYVARVQENIKESKEHIFDTPPTEDPHYIVFEKFQQDVH
GPVLERIKAGRSKLTEPSAQQANGGHATGLSWVKEGEFKPLSIE*

HL08032.pep Blast Records

Blast to dana-all-translation-r1.3.fasta performed 2014-11-27 12:02:33
Subject Length Description Subject Range Query Range Score Percent Strand
Dana\GF13050-PA 242 GF13050-PA 1..242 1..244 1158 86.9 Plus
Dana\GF13159-PA 266 GF13159-PA 11..211 16..209 495 44.1 Plus
Blast to dere-all-translation-r1.3.fasta performed 2014-11-27 12:02:34
Subject Length Description Subject Range Query Range Score Percent Strand
Dere\GG25253-PA 244 GG25253-PA 1..244 1..244 1249 95.1 Plus
Dere\GG20882-PA 266 GG20882-PA 11..211 16..209 502 45 Plus
Blast to dgri-all-translation-r1.3.fasta performed 2014-11-27 12:02:36
Subject Length Description Subject Range Query Range Score Percent Strand
Dgri\GH20212-PA 246 GH20212-PA 1..246 1..244 880 70 Plus
Dgri\GH22748-PA 211 GH22748-PA 6..206 9..205 536 49.3 Plus
Blast to dmel-all-translation-r6.02.fasta performed 2014-11-27 12:02:32
Subject Length Description Subject Range Query Range Score Percent Strand
cbx-PB 244 CG10536-PB 1..244 1..244 1290 100 Plus
cbx-PA 214 CG10536-PA 1..214 31..244 1139 100 Plus
cbx-PC 183 CG10536-PC 1..183 31..244 937 85.5 Plus
CG16894-PA 266 CG16894-PA 11..211 16..209 492 45.5 Plus
Blast to dmoj-all-translation-r1.3.fasta performed 2014-11-27 12:02:37
Subject Length Description Subject Range Query Range Score Percent Strand
Dmoj\GI20739-PA 245 GI20739-PA 1..245 1..244 975 72.1 Plus
Dmoj\GI18531-PA 256 GI18531-PA 13..213 16..210 552 47.8 Plus
Blast to dper-all-translation-r1.3.fasta performed 2014-11-27 12:02:38
Subject Length Description Subject Range Query Range Score Percent Strand
Dper\GL10235-PA 244 GL10235-PA 1..244 1..244 1128 83.2 Plus
Dper\GL11711-PA 262 GL11711-PA 13..228 18..220 487 43.5 Plus
Blast to dpse-all-translation-r3.2.fasta performed 2014-11-27 12:02:39
Subject Length Description Subject Range Query Range Score Percent Strand
Dpse\GA10380-PA 244 GA10380-PA 1..244 1..244 1128 83.2 Plus
Dpse\GA10380-PB 214 GA10380-PB 1..214 31..244 981 81.8 Plus
Dpse\GA14202-PA 262 GA14202-PA 13..228 18..220 481 43.1 Plus
Blast to dsec-all-translation-r1.3.fasta performed 2014-11-27 12:02:40
Subject Length Description Subject Range Query Range Score Percent Strand
Dsec\GM20572-PA 244 GM20572-PA 1..244 1..244 1274 97.5 Plus
Dsec\GM19805-PA 266 GM19805-PA 11..211 16..209 492 45.5 Plus
Blast to dsim-all-translation-r1.4.fasta performed 2014-11-27 12:02:41
Subject Length Description Subject Range Query Range Score Percent Strand
Dsim\GD10043-PA 244 GD10043-PA 1..244 1..244 1283 98.4 Plus
Dsim\GD25298-PA 188 GD25298-PA 11..151 16..156 328 46.5 Plus
Blast to dvir-all-translation-r1.2.fasta performed 2014-11-27 12:02:42
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\GJ20476-PA 245 GJ20476-PA 1..245 1..244 966 71.7 Plus
Dvir\GJ21397-PA 256 GJ21397-PA 13..212 16..209 562 49 Plus
Blast to dwil-all-translation-r1.3.fasta performed 2014-11-27 12:02:43
Subject Length Description Subject Range Query Range Score Percent Strand
Dwil\GK21357-PA 251 GK21357-PA 1..251 1..244 1015 75.7 Plus
Dwil\GK15683-PA 273 GK15683-PA 11..211 16..208 556 48.8 Plus
Blast to dyak-all-translation-r1.3.fasta performed 2014-11-27 12:02:44
Subject Length Description Subject Range Query Range Score Percent Strand
Dyak\GE21973-PA 244 GE21973-PA 1..244 1..244 1246 95.1 Plus
Dyak\GE13821-PA 266 GE13821-PA 11..211 16..209 491 44.6 Plus

HL08032.hyp Sequence

Translation from 74 to 808

> HL08032.hyp
MTLDLDAHKKDDKLLITTIQQEYKILAEYKMIESEKLSGIYVIPSYANSL
QWFGVFFGRQGLYAESVFRFTILLPDRFPDDKSLPSIIFQQDVIHPHVCP
YTHSLDVSHAFPEWRCGEDHLWQLLKYLQVIFSDPLDSIRGIEVDKLKNS
EAAELLMNNKEEYVARVQENIKESKEHIFDTPPTEDPHYIVFEKFQQDVH
GPVLERIKAGRSKLTEPSAQQANGGHATGLSWVKEGEFKPLSIE*

HL08032.hyp Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 10:05:25
Subject Length Description Subject Range Query Range Score Percent Strand
cbx-PB 244 CG10536-PB 1..244 1..244 1290 100 Plus
cbx-PA 214 CG10536-PA 1..214 31..244 1139 100 Plus
cbx-PC 183 CG10536-PC 1..183 31..244 937 85.5 Plus
CG16894-PA 266 CG16894-PA 11..211 16..209 492 45.5 Plus