Clone LD21601 Report

Search the DGRC for LD21601

Clone and Library Details

Library:LD
Tissue Source:Drosophila melanogaster embryo
Created by:Ling Hong
Date Registered:1997-12-04
Comments:Constructed using Stratagene ZAP-cDNA Synthesis kit. Oligo dT-primed and directionally cloned at EcoRI and XhoI in BlueScript SK(+/-)
Original Plate Number:216
Well:1
Vector:pOT2
Associated Gene/TranscriptalphaSnap-RA
Protein status:LD21601.pep: gold
Preliminary Size:2200
Sequenced Size:2054

Associated Genes

Associations are from manual ordering of a clone or by a periodic analysis.
Gene Date Evidence
CG6625 2001-01-01 Release 2 assignment
CG6625 2003-01-01 Sim4 clustering to Release 3
CG6625 2003-01-14 Blastp of sequenced clone
Snap 2008-04-29 Release 5.5 accounting
Snap 2008-08-15 Release 5.9 accounting
Snap 2008-12-18 5.12 accounting

Clone Sequence Records

LD21601.complete Sequence

2054 bp (2054 high quality bases) assembled on 2003-01-14

GenBank Submission: AY069477

> LD21601.complete
CACCAAGTCAGCATATTGATTTTATTTTCTCGAAAAAAGATCCCAATACG
TACACACACTCGCTGAAAAGCCGAACAAGTCTTAAACTTAGATGCAATTA
GTGCCGAGGCGGACATAAATCCTCGACATGGGTGACAACGAACAGAAGGC
GCTCCAGCTGATGGCCGAGGCGGAGAAGAAGTTGACCCAGCAGAAGGGCT
TTCTGGGATCGCTGTTCGGAGGGTCCAACAAGGTGGAGGACGCCATCGAG
TGCTACCAGCGGGCGGGCAACATGTTTAAGATGTCCAAAAACTGGACAAA
GGCTGGGGAGTGCTTCTGCGAGGCGGCAACTCTACACGCGCGGGCTGGAA
GTCGTCACGATGCCGGAACCTGCTACGTAGACGCTTCCAACTGCTACAAG
AAGGTGGACGTGGAGAGCGCCGTCAACTGCCTGATGAAGTCCATCGACAT
CTACACGGATATGGGCCGCTTTACGATGGCTGCCAAACATCACCAAAGCA
TCGCCGAGATGTACGAAAGTGATCCCAATAATCTGGCCAAGTCTATTCAG
CACTATGAGCAGGCGGCTGATTACTTCAAGGGTGAAGAGTCGGTCAGTTC
GGCCAACAAGTGCATGTTGAAGGTGGCCCAGTACGCCGCCCAACTGGAGG
ATTACGAGAAAGCAATATCCATATACGAGCAGGTGGCCGCCTCCTCGCTG
GAGAGCTCTCTGCTCAAGTACAGTGCCAAGGAGTACTTCTTCAGGGCCGC
TCTATGCCATCTCAGCGTGGACCTCCTGAACGCCCAGCATGCCATCGAAA
AGTATGCGCAGCAGTACCCAGCATTCCAGGACTCCAGGGAGTTCAAGCTC
ATCAAGGTGTTGTGCGAGAATCTAGAGGAACAGAACATCGAGGGATTCAC
AGAGGCCGTCAAGGACTACGACAGCATCTCCCGGTTGGATCAGTGGTACA
CCACCATCTTGCTGCGGATCAAGAAGGCTGCGGACGAGGATCCCGATCTG
CGATAAACGAAATCAATTAAATTTTCGGATAAAATCGCGTACACATTTAT
TAAATGCTTTATTCGGTTCGGATATTAATCTACCTATACATATATAACTA
AAAGAAAATTATTAAACGTGTTGCATTGCTTAACCTGAAAGCACCCAAAC
GACGGCCAGCTTGTACGCGTGAATTAAACCTATGGAATAGTCTATATTCC
CGTATTCTAGAGTTTAATTTAACGTTTATGCAACGAATGAAGAAGGCAGC
AGATGCCGAGGCTCAAATTGCGCTACCACGTGCTATTCAAAAGAAAAACT
TTATTATTTCCAATGTGGAGTGATTAGAGGTGTTACTTATGTTTGTCTGT
TTTTGCGTTACTCTTGTTATTTGTGTAAGTAGCCAATCAATCAGTGGGTT
AGCTGATGTTCCATTTGGATGCGTAAAGTACCCTATAGAAATCTGTATCC
ACTACTATAATAAATTGAGAACTTCATTCTATGTGTGTAATGTGTTAGAT
AATTTTGAGATTAACTAAAGAGTCCCTGGTAGCGAAAATGAATTTCAAAC
CAATTATAATTCCATTTAGTGCACTGAAATAAAATTAAACCTAAAGTTTA
GATTGTACGCATATACACAACCTACAAATAAAATATATGTCCACATAAAC
ATTACCGATTACCCCGATAAATATCCAAAAAGTACACGGAATTCTAATAA
ATACTTGAGGGTCACATTTATAGAAAATTCCATCTCGCAAAATTTTAACG
ATGTTTAGTAGATCAACATGTACGAGCGAATTATAATCCAATAAATAATA
TTACGACGGAGCGACGAAGATTACGCGGTCGTCGAGGCCGAAGCAAGAAT
AAATAAATCAATTATTGAATTTATTGAATGAAGATCTCCAGGAAACACAC
AACACCACTGAAACCTGACCCAACACATTTAATTATTAATACTGCAAAGA
TCCTGGTAATGCCTCTATGCAAGCGTTACCACGGATATTTCCAATTCAAA
TACTTTAATAAAGTTAACGCTTGGATTAATCCTGTCAAAAAAAAAAAAAA
AAAA

LD21601.complete Blast Records

Blast to MB8.fasta performed 2010-07-15 18:26:45
Subject Length Description Subject Range Query Range Score Percent Strand
Snap-RA 2336 Snap-RA 176..2212 1..2037 10185 100 Plus
Snap.a 1891 Snap.a 69..1887 219..2037 9095 100 Plus
Blast to d_melanogaster_OreR.fa performed 2014-11-26 23:15:07
Subject Length Description Subject Range Query Range Score Percent Strand
chr3L 24539361 chr3L 20304501..20305682 855..2036 5850 99.7 Plus
chr3L 24539361 chr3L 20304087..20304411 535..859 1625 100 Plus
chr3L 24539361 chr3L 20303712..20304029 219..536 1590 100 Plus
chr3L 24539361 chr3L 20302771..20302989 1..219 1065 99.1 Plus
Blast to dmel-all-CDS-r6.02.fasta performed 2014-11-26 23:15:03
Subject Length Description Subject Range Query Range Score Percent Strand
alphaSnap-RA 879 CG6625-PA 1..879 128..1006 4395 100 Plus
Blast to dmel-all-all_noncoding-r5.12.fasta performed on 2010-04-22 19:09:23 has no hits.
Blast to dmel-all-transcript-r6.02.fasta performed 2014-11-26 23:15:02
Subject Length Description Subject Range Query Range Score Percent Strand
alphaSnap-RA 2070 CG6625-RA 35..2070 1..2036 10180 100 Plus
Blast to dmel-all-translation-r6.02.fasta performed 2014-11-26 23:15:06
Subject Length Description Subject Range Query Range Score Percent Strand
alphaSnap-PA 292 CG6625-PA 1..292 128..1003 1513 100 Plus
Blast to na_all.dmel.RELEASE6 performed 2014-11-26 23:15:00
Subject Length Description Subject Range Query Range Score Percent Strand
3L 28110227 3L 20315396..20316578 855..2037 5915 100 Plus
3L 28110227 3L 20314982..20315306 535..859 1625 100 Plus
3L 28110227 3L 20314607..20314924 219..536 1590 100 Plus
3L 28110227 3L 20313666..20313884 1..219 1095 100 Plus
Blast to na_arms.dmel.RELEASE6 performed 2011-12-12 20:03:49
Subject Length Description Subject Range Query Range Score Percent Strand
3L 28103327 3L 20308496..20309678 855..2037 5915 100 Plus
3L 28103327 3L 20308082..20308406 535..859 1625 100 Plus
3L 28103327 3L 20307707..20308024 219..536 1590 100 Plus
3L 28103327 3L 20306766..20306984 1..219 1095 100 Plus
Blast to na_te.dros performed 2014-11-26 23:15:01
Subject Length Description Subject Range Query Range Score Percent Strand
mdg1 7480 mdg1 DMRTMGD1 7480bp Derived from X59545 (g8507) (Rel. 49, Last updated, Version 4). 6823..6947 1608..1732 129 57.9 Plus

LD21601.complete Sim4 Records

Sim4 to d_melanogaster_OreR.fa performed 2014-11-27 01:11:48 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
chr3L 20304503..20305682 857..2036 99   Plus
chr3L 20304088..20304408 536..856 100 -> Plus
chr3L 20303713..20304028 220..535 100 -> Plus
chr3L 20302771..20302989 1..219 99 -> Plus
Sim4 to dmel-all-CDS-r5.12.fasta performed 2008-12-08 18:56:27 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
Snap-RA 1..879 128..1006 100   Plus
Sim4 to dmel-all-CDS-r5.32.fasta performed 2011-03-16 16:57:24 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
Snap-RA 1..879 128..1006 100   Plus
Sim4 to dmel-all-CDS-r5.52.fasta performed 2013-08-04 06:08:21 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
Snap-RA 1..879 128..1006 100   Plus
Sim4 to dmel-all-CDS-r5.9.fasta performed 2008-07-21 17:48:12 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
Snap-RA 1..879 128..1006 100   Plus
Sim4 to dmel-all-CDS-r6.02.fasta performed 2014-11-27 01:11:48 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
alphaSnap-RA 1..879 128..1006 100   Plus
Sim4 to dmel-all-transcript-r5.12.fasta performed 2008-11-10 20:13:28 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
Snap-RA 33..2068 1..2036 100   Plus
Sim4 to dmel-all-transcript-r5.32.fasta performed 2011-03-16 16:57:23 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
Snap-RA 33..2068 1..2036 100   Plus
Sim4 to dmel-all-transcript-r5.52.fasta performed 2013-08-04 06:08:21 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
Snap-RA 35..2070 1..2036 100   Plus
Sim4 to dmel-all-transcript-r5.9.fasta performed 2008-07-21 17:48:13 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
Snap-RA 33..2068 1..2036 100   Plus
Sim4 to dmel-all-transcript-r6.02.fasta performed 2014-11-27 01:11:48 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
alphaSnap-RA 35..2070 1..2036 100   Plus
Sim4 to na_all.dmel.RELEASE6 performed 2014-11-27 01:11:48 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
3L 20315398..20316577 857..2036 100   Plus
3L 20314983..20315303 536..856 100 -> Plus
3L 20314608..20314923 220..535 100 -> Plus
3L 20313666..20313884 1..219 100 -> Plus
Sim4 to na_arms.dmel.RELEASE5 performed 2013-08-04 06:08:21 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
arm_3L 20308498..20309677 857..2036 100   Plus
arm_3L 20308083..20308403 536..856 100 -> Plus
arm_3L 20307708..20308023 220..535 100 -> Plus
arm_3L 20306766..20306984 1..219 100 -> Plus
Sim4 to na_arms.dmel.RELEASE6 performed 2011-12-09 14:19:36 Download gff for LD21601.complete
Subject Subject Range Query Range Percent Splice Strand
3L 20308498..20309677 857..2036 100   Plus
3L 20308083..20308403 536..856 100 -> Plus
3L 20307708..20308023 220..535 100 -> Plus
3L 20306766..20306984 1..219 100 -> Plus

LD21601.pep Sequence

Translation from 127 to 1005

> LD21601.pep
MGDNEQKALQLMAEAEKKLTQQKGFLGSLFGGSNKVEDAIECYQRAGNMF
KMSKNWTKAGECFCEAATLHARAGSRHDAGTCYVDASNCYKKVDVESAVN
CLMKSIDIYTDMGRFTMAAKHHQSIAEMYESDPNNLAKSIQHYEQAADYF
KGEESVSSANKCMLKVAQYAAQLEDYEKAISIYEQVAASSLESSLLKYSA
KEYFFRAALCHLSVDLLNAQHAIEKYAQQYPAFQDSREFKLIKVLCENLE
EQNIEGFTEAVKDYDSISRLDQWYTTILLRIKKAADEDPDLR*

LD21601.pep Blast Records

Blast to dana-all-translation-r1.3.fasta performed 2014-11-27 22:38:40
Subject Length Description Subject Range Query Range Score Percent Strand
Dana\GF10051-PA 292 GF10051-PA 1..292 1..292 1538 98.3 Plus
Blast to dere-all-translation-r1.3.fasta performed 2014-11-27 22:38:41
Subject Length Description Subject Range Query Range Score Percent Strand
Dere\GG16100-PA 292 GG16100-PA 1..292 1..292 1549 99.3 Plus
Blast to dgri-all-translation-r1.3.fasta performed 2014-11-27 22:38:42
Subject Length Description Subject Range Query Range Score Percent Strand
Dgri\GH14381-PA 292 GH14381-PA 1..292 1..292 1531 97.6 Plus
Blast to dmel-all-translation-r6.02.fasta performed 2014-11-27 22:38:38
Subject Length Description Subject Range Query Range Score Percent Strand
alphaSnap-PA 292 CG6625-PA 1..292 1..292 1513 100 Plus
Blast to dmoj-all-translation-r1.3.fasta performed 2014-11-27 22:38:43
Subject Length Description Subject Range Query Range Score Percent Strand
Dmoj\GI11324-PA 262 GI11324-PA 1..262 1..292 1328 87.3 Plus
Blast to dper-all-translation-r1.3.fasta performed 2014-11-27 22:38:45
Subject Length Description Subject Range Query Range Score Percent Strand
Dper\GL25130-PA 293 GL25130-PA 1..293 1..292 1432 95.6 Plus
Blast to dpse-all-translation-r3.2.fasta performed 2014-11-27 22:38:46
Subject Length Description Subject Range Query Range Score Percent Strand
Dpse\GA19734-PA 293 GA19734-PA 1..293 1..292 1432 95.6 Plus
Blast to dsec-all-translation-r1.3.fasta performed 2014-11-27 22:38:47
Subject Length Description Subject Range Query Range Score Percent Strand
Dsec\GM22271-PA 292 GM22271-PA 1..292 1..292 1545 99 Plus
Blast to dsim-all-translation-r1.4.fasta performed 2014-11-27 22:38:49
Subject Length Description Subject Range Query Range Score Percent Strand
Dsim\GD14872-PA 292 GD14872-PA 1..292 1..292 1545 99 Plus
Blast to dvir-all-translation-r1.2.fasta performed 2014-11-27 22:38:51
Subject Length Description Subject Range Query Range Score Percent Strand
Dvir\GJ11580-PA 292 GJ11580-PA 1..292 1..292 1529 97.6 Plus
Blast to dwil-all-translation-r1.3.fasta performed 2014-11-27 22:38:52
Subject Length Description Subject Range Query Range Score Percent Strand
Dwil\GK16949-PA 302 GK16949-PA 1..289 1..289 1493 96.9 Plus
Blast to dyak-all-translation-r1.3.fasta performed 2014-11-27 22:38:53
Subject Length Description Subject Range Query Range Score Percent Strand
Dyak\GE19660-PA 292 GE19660-PA 1..292 1..292 1542 99 Plus
Dyak\GE19663-PA 412 GE19663-PA 1..204 1..204 1079 99.5 Plus

LD21601.hyp Sequence

Translation from 127 to 1005

> LD21601.hyp
MGDNEQKALQLMAEAEKKLTQQKGFLGSLFGGSNKVEDAIECYQRAGNMF
KMSKNWTKAGECFCEAATLHARAGSRHDAGTCYVDASNCYKKVDVESAVN
CLMKSIDIYTDMGRFTMAAKHHQSIAEMYESDPNNLAKSIQHYEQAADYF
KGEESVSSANKCMLKVAQYAAQLEDYEKAISIYEQVAASSLESSLLKYSA
KEYFFRAALCHLSVDLLNAQHAIEKYAQQYPAFQDSREFKLIKVLCENLE
EQNIEGFTEAVKDYDSISRLDQWYTTILLRIKKAADEDPDLR*

LD21601.hyp Blast Records

Blast to dmel-all-translation-r6.02.fasta performed 2014-11-28 16:26:16
Subject Length Description Subject Range Query Range Score Percent Strand
alphaSnap-PA 292 CG6625-PA 1..292 1..292 1513 100 Plus