Uncharacterized EST or DGC clone means that either this clone has only been end-sequenced, or if it's been completely sequenced either has an error in it or it does not code for a complete full-length ORF. Please review the Flybase and genbank records associated with the clone to determine if this clone will meet your needs.
BDGP iPCR Collection
The BDGP iPCR Collection contains cDNA clones generated by BDGP using a screening method called SLIP (Self-Ligation of Inverse PCR Products). This method is utilized to recover full-length cDNAs for relatively rare and alternatively spliced transcripts of interest. To date, this collection contains 981 genes not previously represented in the EST or DGC collections (Hoskins et al., 2005).
Orders from the BDGP iPCR collection are currently shipped approximately 7 - 14 days after the date the order was first placed. If you order more than five (5) clones at a time, your shipment will be further delayed.
BDGP Gold cDNA Collection
The BDGP Gold Collection is a subset of the Drosophila Gene Collection (DGC). This collection is the most highly validated set of cDNAs available.It contains over 8,000 clones that are full length according to the annotation of the gene. We have received these clones "as is" from the BDGP.
There may be GOLD clones listed on BDGP's website that the DGRC does not have. It is our understanding that these plates will eventually be sent to us.
Orders from the BDGP Gold Collection are currently shipped approximately 7 - 14 days after the date the order was first placed. If you order more than five (5) clones at a time, your shipment will be further delayed.
ECD Collection
The extracellular domain (ECD) collection consists of a set of 202 extracellular domains of Drosophila proteins composed of the immunoglobulin superfamily (IgSF), fibronectin type III (FnIII), and leucine-rich repeat (LRR) families. They were each individually cloned into two complementary bait and prey vectors - to create a library of 404 clones - that permits expression and secretion from Drosophila cell lines. See Ozkan et al., 2003 for more information.
FlyBi ORFeome Collection
The FlyBi ORFeome Collection contains 10,271 open reading frames (ORFs) from D. melanogaster, cloned in the pDONR223 Gateway vector. These represent 2/3 of the proteome. Visit FlyBi at HMS for more information.
CuraGen Yeast 2-Hybrid Collection
CuraGen Corporation, in collaboration with researchers at the Johns Hopkins University, Wayne State University School of Medicine and Yale University School of Medicine, completed the first comprehensive protein interaction map for a multicellular organism, Drosophila melanogaster, and published the results in the online edition and the December 5, 2003 print edition of Science.
The research used the yeast two-hybrid protocol in a high-throughput mode to capture a significant fraction of the organism's protein-protein interaction.
CuraGen Materials available through the DGRC
The following CuraGen materials are available through the DGRC:
- 10,787 AD clones in CuraGen yeast line N106r
- 10,623 BD clones in CuraGen yeast lines YULH
Both the AD and BD clones have been QC'ed by sizing and sequencing in the 5' direction.
The vectors used to make these clones were pBD-Gal4 CAM ("BD" from Stratagene) and pAct2 ("AD" from BD Biosciences).
The yeast 2-hybrid clones were generated using the primers designed based on release 1 and 2. The yeast 2-hybrid Primer File is available to download and should be used as reference only. It is our understanding that Curagen only kept track of the gene identity by sequencing once the clones were generated; not based on the gene intended to be amplified by the primers listed.
Tagged Transcription Factor BAC Collection
The Tagged Transcription Factor BAC Collection contains 650 clones in attB-BAC for Drosophila transgenics (Dr. Kevin White, unpublished). These are BAC clones containing Drosophila transcription factors in which recombineering was utilized to add an epitope tag to the carboxy end of the open reading frame. The transcription factor is under the control of its endogenous promoter sequences and the BACs contain an attB site for high efficiency integration into the Drosophila genome.
Dr. Kevin White has provided a spreadsheet with information on each clone.
Weizmann gRNA Collection
This collection comes from the Weizmann Knock Out project (used to create the "WKO lines") as described in Meltzer et al., 2019. It contains ~850 plasmids with gRNA sequences cloned into pCFD4 or pCFD5. Selection of genes for this gRNA library were made based on expression data of developing MB-gamma neurons.
Phosphoinositide signaling gRNA Library
Phosphoinositide signaling gRNA library was created by the Padinjat Lab (Trivedi et al., 2020). The library contains two sgRNAs cloned into either pBFv6.2 and pBFv6.2B vectors targeting each of the 103 Phosphoinositide signaling genes. For every gene, one sgRNA targets the first coding exon (sg1, cloned into pBFv6.2) and another targets the last coding exon (sg2, cloned into pBFv6.2B). For every gene targeted in the collection, dual gRNA plasmids are also available, that carry both the sgRNA's (sg1 and sg2) in a single plasmid (pBFv6.2B). The gRNA library has been validated for the ability to generate deletions in S2R+ cells and flies.
Drosophila Species ESTs/cDNAs
The Drosophila Species Collections comprise ESTs/cDNAs from five different
species libraries generated by AgenCourt as part of the Drosophila Species
Consortium genome sequencing project. The libraries include:
D. virilis
D. ananassae
D. mojavensis
D. erecta
D. grimshawi
These clones have not been fully characterized and may or may not be full length. We have received these clones "as is" from the AgenCourt.
Species Clone Search
To find if a Drosophila species clone is available, use the DGRC product search to look for the species gene ( e.g. "Dana\GF22818" or "FBgn0099812") or the Dm homolog ( e.g. "ey" or "FBgn0005558"). Available Drosophila species clones will be listed after clicking on the "Dros Gene" results.
ORF driven by a UAS promoter and with a Flag-HA tag C-terminal fusion
BDGP Tagged ORF Collection
This is a collection of expression-ready ORFs that have been tagged. This BDGP collection is still growing. Anything listed as "unavailable" means that we have not received it from BDGP yet. A complete list of what is expected to be available in the future can be found here.
There are seven categories of tagged ORFS:
- UFO - UAS promoter with Flag-HA tag C-terminal fusion
- MXO - metallothionein promoter with TAP tag C-terminal fusion
- FMO - metallothionein promoter with Flag-HA tag C-terminal fusion
- UGS - GFP tag N-terminal fusion
- URS - mCherry tag N-terminal fusion
- UGO - GFP tag C-terminal fusion
- URO - mCherry tag C-terminal fusion
For further information on how these were cloned, please visit the BDGP website.
Extra information from BDGP on the FMO clones is available in the FMO clone FAQ section.
ORF driven by a UAS promoter and with a Flag-HA tag C-terminal fusion
BDGP Tagged ORF Collection
This is a collection of expression-ready ORFs that have been tagged. This BDGP collection is still growing. Anything listed as "unavailable" means that we have not received it from BDGP yet. A complete list of what is expected to be available in the future can be found here.
There are seven categories of tagged ORFS:
- UFO - UAS promoter with Flag-HA tag C-terminal fusion
- MXO - metallothionein promoter with TAP tag C-terminal fusion
- FMO - metallothionein promoter with Flag-HA tag C-terminal fusion
- UGS - GFP tag N-terminal fusion
- URS - mCherry tag N-terminal fusion
- UGO - GFP tag C-terminal fusion
- URO - mCherry tag C-terminal fusion
For further information on how these were cloned, please visit the BDGP website.
Extra information from BDGP on the FMO clones is available in the FMO clone FAQ section.
ORF driven by a UAS promoter and with a Flag-HA tag C-terminal fusion
BDGP Tagged ORF Collection
This is a collection of expression-ready ORFs that have been tagged. This BDGP collection is still growing. Anything listed as "unavailable" means that we have not received it from BDGP yet. A complete list of what is expected to be available in the future can be found here.
There are seven categories of tagged ORFS:
- UFO - UAS promoter with Flag-HA tag C-terminal fusion
- MXO - metallothionein promoter with TAP tag C-terminal fusion
- FMO - metallothionein promoter with Flag-HA tag C-terminal fusion
- UGS - GFP tag N-terminal fusion
- URS - mCherry tag N-terminal fusion
- UGO - GFP tag C-terminal fusion
- URO - mCherry tag C-terminal fusion
For further information on how these were cloned, please visit the BDGP website.
Extra information from BDGP on the FMO clones is available in the FMO clone FAQ section.
ORF driven by a UAS promoter and with a Flag-HA tag C-terminal fusion
BDGP Tagged ORF Collection
This is a collection of expression-ready ORFs that have been tagged. This BDGP collection is still growing. Anything listed as "unavailable" means that we have not received it from BDGP yet. A complete list of what is expected to be available in the future can be found here.
There are seven categories of tagged ORFS:
- UFO - UAS promoter with Flag-HA tag C-terminal fusion
- MXO - metallothionein promoter with TAP tag C-terminal fusion
- FMO - metallothionein promoter with Flag-HA tag C-terminal fusion
- UGS - GFP tag N-terminal fusion
- URS - mCherry tag N-terminal fusion
- UGO - GFP tag C-terminal fusion
- URO - mCherry tag C-terminal fusion
For further information on how these were cloned, please visit the BDGP website.
Extra information from BDGP on the FMO clones is available in the FMO clone FAQ section.
ORF driven by a UAS promoter and with a Flag-HA tag C-terminal fusion
BDGP Tagged ORF Collection
This is a collection of expression-ready ORFs that have been tagged. This BDGP collection is still growing. Anything listed as "unavailable" means that we have not received it from BDGP yet. A complete list of what is expected to be available in the future can be found here.
There are seven categories of tagged ORFS:
- UFO - UAS promoter with Flag-HA tag C-terminal fusion
- MXO - metallothionein promoter with TAP tag C-terminal fusion
- FMO - metallothionein promoter with Flag-HA tag C-terminal fusion
- UGS - GFP tag N-terminal fusion
- URS - mCherry tag N-terminal fusion
- UGO - GFP tag C-terminal fusion
- URO - mCherry tag C-terminal fusion
For further information on how these were cloned, please visit the BDGP website.
Extra information from BDGP on the FMO clones is available in the FMO clone FAQ section.
ORF driven by a UAS promoter and with a Flag-HA tag C-terminal fusion
BDGP Tagged ORF Collection
This is a collection of expression-ready ORFs that have been tagged. This BDGP collection is still growing. Anything listed as "unavailable" means that we have not received it from BDGP yet. A complete list of what is expected to be available in the future can be found here.
There are seven categories of tagged ORFS:
- UFO - UAS promoter with Flag-HA tag C-terminal fusion
- MXO - metallothionein promoter with TAP tag C-terminal fusion
- FMO - metallothionein promoter with Flag-HA tag C-terminal fusion
- UGS - GFP tag N-terminal fusion
- URS - mCherry tag N-terminal fusion
- UGO - GFP tag C-terminal fusion
- URO - mCherry tag C-terminal fusion
For further information on how these were cloned, please visit the BDGP website.
Extra information from BDGP on the FMO clones is available in the FMO clone FAQ section.
ORF driven by a UAS promoter and with a Flag-HA tag C-terminal fusion
BDGP Tagged ORF Collection
This is a collection of expression-ready ORFs that have been tagged. This BDGP collection is still growing. Anything listed as "unavailable" means that we have not received it from BDGP yet. A complete list of what is expected to be available in the future can be found here.
There are seven categories of tagged ORFS:
- UFO - UAS promoter with Flag-HA tag C-terminal fusion
- MXO - metallothionein promoter with TAP tag C-terminal fusion
- FMO - metallothionein promoter with Flag-HA tag C-terminal fusion
- UGS - GFP tag N-terminal fusion
- URS - mCherry tag N-terminal fusion
- UGO - GFP tag C-terminal fusion
- URO - mCherry tag C-terminal fusion
For further information on how these were cloned, please visit the BDGP website.
Extra information from BDGP on the FMO clones is available in the FMO clone FAQ section.
ORF containing native stop codon
BDGP ORF Collection
This collection contains proteomic clones from BDGP. BDGP is transferring 6,500 high-quality ORFs into the BD Clonetech In-Fusion system. The ORFs originate from clones in the Gold Collection. These clones are being sent to the DGRC in subsets as they are completed, rearrayed and verified.
Two clones of each ORF exist, one contains the native stop codon and is referred to as ExpressR-NativeStop(NS) clone and one lacks the stop codon and is referred to as theExpressR-Open(O) clone. The NS and O forms of the clones will allow one to create amino-terminal or carboxy-terminal fusion proteins, respectively. For more information on how this collection is being made, go to the BDGP Universal Proteomics Resources page.
These expression clones are cloned into pDNR-Dual from Clonetech.
ORF with native stop codon removed
BDGP ORF Collection
This collection contains proteomic clones from BDGP. BDGP is transferring 6,500 high-quality ORFs into the BD Clonetech In-Fusion system. The ORFs originate from clones in the Gold Collection. These clones are being sent to the DGRC in subsets as they are completed, rearrayed and verified.
Two clones of each ORF exist, one contains the native stop codon and is referred to as ExpressR-NativeStop(NS) clone and one lacks the stop codon and is referred to as theExpressR-Open(O) clone. The NS and O forms of the clones will allow one to create amino-terminal or carboxy-terminal fusion proteins, respectively. For more information on how this collection is being made, go to the BDGP Universal Proteomics Resources page.
These expression clones are cloned into pDNR-Dual from Clonetech.
Batch Query Results
| Clone | Barcode | FlyBase | Features |
|---|---|---|---|
| RE64054 | 9680 | FBcl0218641 | Uncharacterized EST or DGC clone |
